Effects of barrier function of the intestinal mucosa in premature infants

早产儿肠黏膜屏障功能的影响

• 批准号: 20591307
• 负责人: SHIMIZU Toshiaki
• 金额: $ 3万
• 依托单位: Juntendo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591307/
• 关键词: 新生児医学; 栄養学; 消化器病学; 低出生体重児; 粘膜バリヤー; n-3系PUFA; プロバイオティクス; DHA; 母乳; B.breve; bacterial translocation

To evaluate effects of n-3 polyunsatulated fatty acids (n-3 PUFAs) and probiotics on intestinal mucosal barrier function we performed this study.Fortification of Docosahexaenoic fatty acid (DHA) increased levels of DHA in breast milks of lactated mothers and in brain tissues of rat pups. These results suggest that administration of DHA to mothers may play an important role in the intestinal mucosal barrier function of their infants because n-3 PUFAs is known to contribute to the mucosal barrier function.Administration of Bifidobacterium breve (B.breve) to newborn rats for 2 weeks attenuated the expression of inflammatory molecules, including lipoprotein lipase, glutathione peroxidase 2, lipopolysaccharide binding protein, in the intestinal mucosa using microarray. These findings indicate that probiotics may protect the intestinal mucosal barrier function by suppressing the mucosal inflammationIn low birth weight infants with neonatal sepsis, Enterobacteriaceae was detected in the blood as the most common bacteria using bacterial ribosomal RNA-targeted reverse transcription-quantitaive PCR that was not detected by the conventional blood culture. These results suggest that suppression of the bacterial translocation is needed to prevent neonatal sepsis in low birth weight infants.

为了评估N-3多核脂肪酸（N-3 PUFAS）和益生菌对肠粘膜屏障功能的影响，我们进行了这项研究。docosahexaenoic脂肪酸（DHA）的舒适度增加了乳房母亲乳房乳毛的DHA水平，使乳房母亲的乳房和大脑幼虫组织中的乳房​​乳液中的乳房水平提高。 These results suggest that administration of DHA to mothers may play an important role in the intestinal mucosal barrier function of their infants because n-3 PUFAs is known to contribute to the mucosal barrier function.Administration of Bifidobacterium breve (B.breve) to newborn rats for 2 weeks attenuated the expression of inflammatory molecules, including lipoprotein lipase, glutathione peroxidase 2,脂多糖结合蛋白，使用微阵列在肠粘膜中。 These findings indicate that probiotics may protect the intestinal mucosal barrier function by suppressing the mucosal inflammationIn low birth weight infants with neonatal sepsis, Enterobacteriaceae was detected in the blood as the most common bacteria using bacterial ribosomal RNA-targeted reverse transcription-quantitaive PCR that was not detected by the conventional blood culture.这些结果表明，需要抑制细菌易位以预防低出生体重婴儿的新生儿败血症。

项目成果

細菌 ribosomal RNAを標的としたRT-PCR法による新生児敗血症の診断

使用针对细菌核糖体 RNA 的 RT-PCR 诊断新生儿败血症

• 发表时间: 2010
• 作者: 藤森誠;清水俊明;他
• 通讯作者: 他

新生児壊死性腸炎の予防を見据えたprobaioticsおよびn-3系多価不飽和脂肪酸投与について.

益生菌和 n-3 多不饱和脂肪酸的施用用于预防新生儿坏死性小肠结肠炎。

• 发表时间: 2008
• 期刊: 周産期シンポジウム 26
• 作者: 大塚宜一;清水俊明.
• 通讯作者: 清水俊明.

Fatty acid composition of the brain of intrauterine growth retardation rats and the effect of maternal docosahexaenoic acid enriched diet.

宫内生长迟缓大鼠大脑的脂肪酸组成和母体富含二十二碳六烯酸饮食的影响。

• 发表时间: 2009
• 期刊: Early Hum Dev. 85(12)
• 作者: Ikeno M.;Okumura A.;Hayakawa M.;et al.
• 通讯作者: et al.

Effects of parenteral lipid emulsion on the long-chain polyunsaturated fatty acid profile in very-low-birth-weight infants.

肠外脂肪乳剂对极低出生体重婴儿长链多不饱和脂肪酸谱的影响。

• 发表时间: 2009
• 作者: Shoji H;Shimizu T;et al.
• 通讯作者: et al.

新生児敗血症におけるBacterial rRNA-targeted RT-Quantitative PCR法の有用性

细菌 rRNA 靶向 RT 定量 PCR 方法在新生儿败血症中的应用

• 发表时间: 2010
• 作者: 藤森誠;久田研;et al.
• 通讯作者: et al.