The elucidation of the onset organization of the trigeminal neuralgia using a trigeminal nerve damage animal.

使用三叉神经损伤动物阐明三叉神经痛的发病组织。

• 批准号: 20791562
• 负责人: ABE Tetsuya
• 金额: $ 2.75万
• 依托单位: Hyogo College of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20791562/
• 关键词: 三叉神経; 機械刺激受容ニューロン; サイトカイン; サブスタンスP; GABAAc-Fos; 三叉神経 主感覚核; GABAA; c-Fos; ニューロキニン1; SPサポリン; ニユーロン; ビククリン; GABA受容体; 侵害受容ニューロン

The orofacial region has skin and mucous region and the formalin injection into the two different region induces different nocifensive behavior. The subcutaneous injection of diluted formalin into the rat upper lip induced more nocifensive behaviour than into the tongue. The behavior was inhibited more by the preadministration of an antagonist of GABA_A receptor, bicuculline, when formalin was injected into the lip than into the tongue. The resultant differences of nocifensive behaviour and pharmacology could be caused by the differences in the quantitative and qualitative distribution of C-fiber population. The tongue has fewer primary afferent neurons (trigeminal nerve) in the range of C-fiber than the lip skin.Considerable number of neurons-bearing receptors of substance P (SP), neurokinin-1 (NK-1) are distributed in lamina I and less in lamina III of trigeminal subnucleus caudalis (Vc). We have eliminated these NK-1-bearing neurons by preadministration of SP conjugated with a ribosome-inactivating toxin, saporin (SP-Sap) into the rat cisterna magna. In these SP-Sap-pretreated rats, the formalin-induced nocifensive behaviour was reduced and the preadministration of bicuculline enhanced the nocifensive behaviour, when bicuculline reduced it in the naive rats. These results show that NK-1-bearing neurons are involved in pro-nociceptive transducer through ascending and descending nociceptive facilitating loop, where GABA acts as prpnociceptive.

口面区域具有皮肤和粘液区域，福尔马林注射到两个不同的区域会诱导不同的单化行为。皮下注射稀释后的福尔马林向大鼠上唇诱发的行为比舌头更具单一的行为。当将福尔马林注入嘴唇而不是舌头时，通过对GABA_A受体Bicuculline的拮抗剂的预分泌物抑制了这种行为。由于c纤维人群的定量和定性分布的差异，单位行为和药理学的结果差异可能是引起的。舌头在C纤维范围内的原发性传入神经元（三叉神经）少于唇部皮肤。物质P（SP）的含神经元的受体数量较多，Neurokinin-1（NK-1）分布在层中I在三叉神经库氏菌（VC）的椎板III中较少。我们已经通过与核糖体灭活的毒素，saporin（SP-SAP）结合的SP进行前分泌来消除这些含NK-1的神经元。在这些SP-SAP预测的大鼠中，福尔马林诱导的单位性行为降低了，当双颈降低在幼稚的大鼠中时，双瓜氨酸的预分泌增强了单位性行为。这些结果表明，含有NK-1的神经元通过上升和下降的伤害性促进循环参与亲人感人换能器，其中GABA充当PropNoctocative。

项目成果

三叉神経慢性切断ラットの三叉神経節電気刺激が誘導する三叉神経感覚核のc-Fos発現

电刺激三叉神经节诱导慢性三叉神经横断大鼠三叉神经感觉核中c-Fos的表达

• 发表时间: 2009
• 作者: 阿部徹也;本田公亮;山村倫世;藤原正識;浦出雅裕;奥井森
• 通讯作者: 奥井森

Effects of systemic bicuculline or morphine on formalin-evoked pain-related behaviour and c-Fos expression in trigeminal nuclei after formalin injection into the lip or tongue in rats

• DOI: 10.1007/s00221-009-1842-1
• 发表时间: 2009-05
• 期刊: Experimental Brain Research
• 影响因子: 2
• 作者: S. Sugiyo;D. Uehashi;F. Satoh;Tetsuya Abe;Norifumi Yonehara;Masayuki Kobayashi;M. Takemura

ラットにおける下歯槽神経、眼窩下神経切断後に三叉神経節への電気刺激が誘導する三叉神経感覚核の異常なc-Fos発現

大鼠下牙槽神经和眶下神经横断后电刺激三叉神经节致三叉神经感觉核c-Fos异常表达

• 发表时间: 2009
• 作者: 阿部徹也;本田公亮;山村倫世;奥井森;浦出雅裕
• 通讯作者: 浦出雅裕

Neurokinin-1-receptor bearing neurons in the trigeminal caudal nucleus are crucial for intensity-dependent bidirectional sensory processing through GABAA receptors

三叉神经尾核中带有 Neurokinin-1 受体的神经元对于通过 GABAA 受体进行强度依赖性双向感觉处理至关重要

• 发表时间: 2008
• 作者: 阿部徹也;本田公亮;山村倫世;藤原正識;浦出雅裕;奥井森;阿部徹也
• 通讯作者: 阿部徹也

Effect of systemic bicuculine or morphine on formarin-evoked pain-related behaviour and c-Fos expression in trigeminal nuclei after formalin injection into the lip or tongue in rats.

福尔马林注射到大鼠唇或舌后，全身荷包牡丹碱或吗啡对福尔马林诱发的疼痛相关行为和三叉神经核 c-Fos 表达的影响。

• 发表时间: 2009
• 期刊: Exp Brain Res 196
• 作者: S. Sugiyo;D. Uehashi;F. Satoh;T. Abe;N. Yonehara;M. Kobayashi;Motohide Takemura.
• 通讯作者: Motohide Takemura.