Identification of regulators controlling cellulase and hemicellulase gene expression in filamentous fungi and its functional analysis.

丝状真菌纤维素酶和半纤维素酶基因表达调控因子的鉴定及其功能分析。

• 批准号: 20780058
• 负责人: TANI Shuji
• 金额: $ 2.75万
• 依托单位: Osaka Prefecture University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20780058/
• 关键词: Aspergillus aculeatus; (hemi)cellulase; gene regulation; Agrobacterium tumefaciens-mediated transformation; TAIL-PCR; Insertional mutagenesis; Agrobacterium tumefaciens; Transformation; Gene tagging; Cellulase; Gene regulation; Agrobacterium

We report the successful Agrobacterium tumefaciens-mediated transformation (AMT) of Aspergillus aculeatus for functional genomics. We isolated 40 transformants expected to be disruptants of the regulator(s) controlling cellulase gene expressions in A. aculeatus from 15,000 transformants obtained by AMT. Mutation of the geneA resulted in 5-fold reduction on cellobiohydrolase gene expression in transformant by comparing with that in host strain. Now, we are further analyzing the function of putative transcription factor identified in this study and seeking the other regulators.

我们报告了曲霉曲霉的成功农杆菌介导的曲霉菌曲霉（AMT）用于功能基因组学。我们分离了40种变换物，预计将是从AMT获得的15,000个转化体中控制纤维素酶基因表达的调节剂的破坏因子。基因的突变通过与宿主菌株中的相比，导致转化剂中的纤维二氢酶基因表达降低了5倍。现在，我们正在进一步分析本研究中确定的推定转录因子的功能，并寻求其他调节剂。

项目成果

Aspergillus aculeatusにおける網羅的遺伝子破壊株の作出を目的としたアグロバクテリウム形質転換法の開発

开发用于生产棘孢曲霉综合基因破坏菌株的农杆菌转化方法

• 发表时间: 2008
• 作者: 國武絵美;谷修治;炭谷順一;川口剛司
• 通讯作者: 川口剛司

Development of Agrobacterium tumefaciens-mediated transformation system for random insertional mutaaenesis in Aspergillus aculeatus

开发根癌农杆菌介导的棘孢曲霉随机插入诱变转化系统

• 发表时间: 2008
• 作者: E. Kunitake;S. Tani;J. Sumitani;and T. Kawaguchi
• 通讯作者: and T. Kawaguchi

Physiological inducer and cis-elements required for the cellobiohydrolase lgene induction via XlnR-indenendent pathway in Aspergillus aculeatus

棘孢曲霉中通过 XlnR 独立途径诱导纤维二糖水解酶基因所需的生理诱导剂和顺式元件

• 期刊: Biotechnology of lignocelluloses degradation, biomass utilization, and biorefinery (In press)
• 作者: S. Tani;J. Sumitani;and T. Kawaguchi
• 通讯作者: and T. Kawaguchi

Physiological inducer and cis-elements required for the cellobiohydrolase Igene induction via XlnR-independent pathway in Aspergillus aculeatus.

棘孢曲霉中通过 XlnR 独立途径诱导纤维二糖水解酶 I 基因所需的生理诱导剂和顺式元件。

• 期刊: Biotechnology of lignocelluloses degradation, biomassutilization, and biorefinery. 2009-11
• 作者: S.Tani;J.Sumitani;T.Kawaguchi
• 通讯作者: T.Kawaguchi

Physiological inducer and cis-element required for the cellobiohydrolase I gene induction via XlnR-independent pathway in Aspergillus aculeatus

棘孢曲霉中通过 XlnR 独立途径诱导纤维二糖水解酶 I 基因所需的生理诱导剂和顺式元件

• 发表时间: 2008
• 作者: Shuji Tani;Jun-ichi Sumitani;Takashi Kawaguchi
• 通讯作者: Takashi Kawaguchi