Effect of circulating monocyte subsets and endothelial progenitor cells on coronary plaque rupture

循环单核细胞亚群和内皮祖细胞对冠状动脉斑块破裂的影响

基本信息

批准号：
20590835
负责人：
IMANISHI Toshio
金额：
$ 3万
依托单位：
Wakayama Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2008
资助国家：
日本
起止时间：
2008 至 2010
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20590835/
关键词：
冠動脈プラーク急性冠症候群単球血管内皮前駆細胞 OCT

项目摘要

Monocytes represent a heterogenous circulating population of cells. We showed that dynamic changes occur in 2 distinct monocyte subset levels (CD14^+CD1^6-CCR2^+ and CD14^+CD16^+CX3CR1^+) in patients with acute myocardial infarction, and that stochastic profiling of this monocyte system may have hold clinical utility with respect to cardiovasculardiseases (J Am Coll Cardiol. 2009). Early diagnosis and risk stratification of patients with stable angina pectoris (SAP), or acute coronary syndrome is of utmost importance. We examined the impact of circulating monocyte subset levels on the burden of coronary vulnerable plaque. First, we investigated the relationship between monocyte subsets and coronary plaque vulnerability, such as positive remodeling (remodeling index >1.05) and/or low CT attenuation (<35 HU), assessed by multi-detector computed tomography (MDCT) in 80 patients with SAP. The distinct monocyte subsets were measured by flow cytometry. The relative proportions of CD14^+CD16^+CX3CR1^+ monocytes were significantly higher in patients with coronary vulnerable plaque than in patients without it. These monocyte subpopulations positively correlated with remodeling index, and negatively with CT attenuation value (Atherosclerosis. 2010). Next, we examined the association of monocyte subset counts with coronary fibrous cap thickness (FCT) in 40 patients with unstable angina pectoris. The changes in the non-culprit FCT were assessed by optical coherence tomography (OCT) at baseline and after 9 months. The percent changes in FCT showed significantly negative correlation with the percent changes in CD14^+CD16^+CX3CR1^+ monocytes, but not CD14^+CD16^-CCR2^+monocytes (Atherosclerosis. 2010). In conclusion, CD14+CD16^+CX3CR1^+ monocytes may serve as a novel biomarker for coronary plaque vulnerability.

单核细胞代表一个异体循环细胞群。我们表明，动态变化发生在2个不同的单核细胞子集水平（CD14^+CD1^6-CCR2^+和CD14^+CD16^+CX3CR1^+）的急性心肌梗死患者中，并且该单核细胞系统的随机分析可能具有与黑前的临床实用性有关的临床实用性，可能具有callior的临床效应。稳定心绞痛（SAP）或急性冠状动脉综合征患者的早期诊断和风险分层至关重要。我们研究了循环单核细胞子集水平对冠状动脉脆弱斑块负担的影响。首先，我们研究了单核细胞亚群与冠状斑块脆弱性之间的关系，例如80名SAP患者的多端计算机计算机层析摄影术（MDCT）评估，例如阳性重塑（重塑指数> 1.05）和/或低CT衰减（<35 HU）。通过流式细胞仪测量不同的单核细胞子集。冠状动脉脆弱斑块患者的CD14^+CD16^+CX3CR1^+单核细胞的相对比例明显高于没有其患者的患者。这些单核细胞亚群与重塑指数正相关，并且与CT衰减值负相关（动脉粥样硬化。2010）。接下来，我们研究了40例不稳定心绞痛患者的单核细胞亚群与冠状动脉纤维帽厚度（FCT）的关联。非元素FCT的变化通过基线和9个月后的光学相干断层扫描（OCT）评估。 FCT的变化百分比与CD14^+CD16^+CX3CR1^+单核细胞的百分比变化的百分比变化明显相关，但没有CD14^+CD16^-CCR2^+单核细胞（动脉粥样硬化。2010）。总之，CD14+CD16^+CX3CR1^+单核细胞可以作为冠状斑块脆弱性的新型生物标志物。