Molecular mechanism for divergent interactions in PAS domain proteins

PAS结构域蛋白不同相互作用的分子机制

• 批准号: 23570194
• 负责人: YAMAZAKI Yoichi
• 金额: $ 3.58万
• 依托单位: Nara Institute of Science and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23570194/
• 关键词: 光生物学; 情報伝達; タンパク質間相互作用; 光受容

There are many different functions even in a structurally homologous proteins. It is not clear how they realize these divergent functions on their same protein backbone. To evaluate these molecular divergent mechanism, two different PYP proteins classified as PAS domain proteins have been investigated about their molecular mechanism for structural changes and protein-protein interaction. As the results, (1) solution structure of the interaction protein for PYP has been identified. And then complex formation of this protein and PYP has been successfully identified. This complex was characteristic ring shape composed by hetero octamer. (2) a surface residue on the PYP has been identified as an interaction site. (3) X-ray crystal analysis for this PYP suggested that structural change was introduced around the interaction residue.

即使结构同源的蛋白质也有许多不同的功能。目前尚不清楚它们如何在同一蛋白质骨架上实现这些不同的功能。为了评估这些分子发散机制，研究了两种被归类为 PAS 结构域蛋白的不同 PYP 蛋白，以了解其结构变化和蛋白质-蛋白质相互作用的分子机制。结果，(1)PYP相互作用蛋白的溶液结构已被鉴定。并成功鉴定了该蛋白与PYP形成的复合物。该配合物是由杂八聚体组成的特征性环状结构。 (2) PYP 上的表面残基已被确定为相互作用位点。 (3) 该 PYP 的 X 射线晶体分析表明，在相互作用残基周围引入了结构变化。

项目成果

Analysis of interaction sites on the Photoactive Yellow Protein of Rhodobacter capsulatus

荚膜红杆菌光活性黄色蛋白相互作用位点分析

• 发表时间: 2013
• 作者: Y. Yamazaki; M. Shimada; H. Kamikubo; M. Kataoka
• 通讯作者: M. Kataoka

Effect of N-terminal region to the structure formation of PYP

N端区域对PYP结构形成的影响

• 作者: M. Sakonji; Y. Yamazaki; H. Kamikubo; M. Yamaguchi; M. Kataoka
• 通讯作者: M. Kataoka

Roles of hydrogen bonds around chromophore in Photoactive Yellow Protein studied by OH-deficient cinnamic acid

缺OH肉桂酸研究光活性黄色蛋白中发色团周围氢键的作用

• 作者: M. Narumi; Y. Yamazaki; M. Yamaguchi; H. Kamikubo; M. Kataoka
• 通讯作者: M. Kataoka

X-ray crystal structure analysis of the Photoactive Yellow Protein of Rhodobacter capsulatus

荚膜红杆菌光敏黄色蛋白的X射线晶体结构分析

• 发表时间: 2013
• 作者: H. Matsumoto; Y. Yamazaki; H. Kamikubo; M. Yamaguchi; M. Kataoka.
• 通讯作者: M. Kataoka.

pH effects on the spectral species and the photoreaction in the Photoactive Yellow Protein of Rhodobacter capsulatus.

pH 对荚膜红杆菌光活性黄色蛋白的光谱种类和光反应的影响。

• 发表时间: 2011
• 作者: Y. Yamazaki; M. Sakoguchi; H. Kamikubo;M. Kataoka
• 通讯作者: M. Kataoka