Novel methods of regeneration of tooth using bone marrow cells-aim at order medical treatment-

利用骨髓细胞再生牙齿的新方法-以有序医疗为目标-

• 批准号: 20390501
• 负责人: ISHIKAWA Hiroshi
• 金额: $ 12.4万
• 依托单位: The Nippon Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20390501/
• 关键词: 再生歯; 骨髄; 組織幹細胞; 潅流培養; 歯根膜; エナメル上皮; マイクロCT; RT-PCR; ヒト骨髄; 3胚葉性テラトーマ; 胚子様構造体; 歯胚; エナメル上皮細胞; ETFs; 還流培養; 再生医療; ヒト骨髄幹細胞; 移植; 環流培養; embryotrophic factor; 骨髄幹細胞(stem cell); BALB; CA^<-CSA>マウス; Hspa9b遺伝子; 歯髄; 内エナメル上皮; 微速灌流培養装置

Several cell lines derived from periodontal membrane, dental pulp, inner enamel epithelium and bone marrow of BALB/cA mouse were established. When the enamel epithelial cells were cultured in the growth medium supplemented with the products of human dental pulp cells, the enamel epithelial cells produced ameloblastin. The pellets of ameroblastin producing cells and dental pulp cells were inserted into the collagen gel adjoining each other. Then these constructions were enveloped with human periodontal membrane. Lastly, they were enveloped with collagen sponge and cultured GM supplemented with the ETFs by circumfusion apparatus.We established 27 tissue stem cell lines derived from human bone marrow. Three of them made teratoma when they were transplanted into the subcutis of nude mouse. The stem cells were cultured by hanging drop method with ETFs, the embryoid bodies were developed. The embryoid bodies were brought up with ETFs to the embryo-like structures by circumfusion apparatus. However, there is nothing of the tooth germ structures yet.

建立了多种源自BALB/cA小鼠牙周膜、牙髓、内釉质上皮和骨髓的细胞系。当釉质上皮细胞在补充有人牙髓细胞产物的生长培养基中培养时，釉质上皮细胞产生成釉细胞。将成色蛋白产生细胞和牙髓细胞的沉淀物插入彼此相邻的胶原凝胶中。然后这些结构被人牙周膜包裹。最后，用胶原海绵包裹它们，并通过环流装置培养添加有ETF的GM。我们建立了27个源自人骨髓的组织干细胞系。其中3个移植到裸鼠皮下组织后形成畸胎瘤。采用ETFs悬滴法培养干细胞，形成拟胚体。通过环化装置将拟胚体与 ETF 培养成类胚结构。然而，目前还没有任何牙胚结构的信息。

项目成果

クラウン系ミニブタ下顎頬歯(臼歯)のエックス線解析

冠型小型猪下颌颊齿（臼齿）X线分析

• 发表时间: 2008
• 作者: Koeda S;Takata Y;Satomi N;Goto S;Nagasaka H;Kawamura H;井出吉昭
• 通讯作者: 井出吉昭

ミニブタ胎仔の支配由来血管内皮細胞の血管形成に関する実験

小型猪胎对照血管内皮细胞血管生成实验

• 发表时间: 2009
• 作者: 那須優則;ほか
• 通讯作者: ほか

耳下腺腫瘍細胞株と同時に樹立しえたB-リンパ球の有用性に関する研究

与腮腺肿瘤细胞系同时建立的B淋巴细胞的用途研究

• 发表时间: 2010
• 作者: 川上未有希;石川博;鈴木見奈子;中原貴;田中彰;叉賀泉
• 通讯作者: 叉賀泉

マウスearlvES細胞の甲状腺原基への分化とその機能解析

小鼠早期ES细胞向甲状腺原基的分化及其功能分析

• 发表时间: 2008
• 作者: 小幡亜希子;他;石川真由美
• 通讯作者: 石川真由美

Establishment and characterization of HER2-positive cell line derived from pleural effusion of human breast scirrhous carcinoma

人乳腺癌硬癌胸腔积液HER2阳性细胞系的建立和鉴定

• 发表时间: 2008
• 期刊: Human Cell 21
• 作者: 浅井崇文;他;Tabei I
• 通讯作者: Tabei I