Establishment of embryonic stem cell lines from in vitro matured and in vitro fertilized dog oocytes and their application to regenerative veterinary medicine

狗体外成熟和体外受精卵母细胞胚胎干细胞系的建立及其在再生兽医学中的应用

• 批准号: 20380174
• 负责人: INABA Toshio
• 金额: $ 11.9万
• 依托单位: Osaka Prefecture University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20380174/
• 关键词: 獣医学; トランスレーショナルリサーチ; バイオテクノロジー; 移植・再生医療; 発生・分化; ES細胞; イヌ; iPS細胞; 卵子; 体外成熟; 神経細胞; 骨髄間質細胞; 再生医療; 体外受精; 体外発育; 胚盤胞期胚; 神経幹細胞; コラーゲン・ゲル; 三次元培養

The objective of this study was to establish embryonic stem(ES) cell lines from in vitro matured and fertilized dog oocytes for regenerative veterinary medicine, and the following results were obtained.(1) We successfully enhanced oocyte growth and maturation by controlling the induction of fertile oestrus in immature and dioestrous bitches.(2) The in vitro maturation rate of canine oocytes was improved by embedding and culturing in collagen gels.(3) Canine ES-like cell colonies were formed by culturing inner cell masses collected from blastocysts in uterine horns, and maintained their proliferation and morphology beyond passage 10.(2) Neurons could be induced simply from primate ES cells.(3) Bone marrow stromal cell transplantation caused no complications and could have beneficial effects on functional recovery of dogs with spinal cord injury, and further studies are required to apply canine ES cells for the treatment of these dogs.

本研究的目的是从体外成熟并受精的狗卵母细胞中建立胚胎干细胞系，用于再生兽医学，并获得以下结果。（1）我们成功地通过控制诱导卵母细胞的生长和成熟来促进卵母细胞的生长和成熟。 (2)通过胶原凝胶包埋培养提高犬卵母细胞的体外成熟率。(3)犬卵母细胞通过培养从子宫角囊胚收集的内细胞团形成ES样细胞集落，并在传代10代后保持其增殖和形态。（2）可以简单地从灵长类ES细胞诱导神经元。（3）骨髓基质细胞移植没有引起任何并发症，并且可能对脊髓损伤犬的功能恢复产生有益影响，需要进一步研究应用犬ES细胞来治疗这些犬。

项目成果

Analysis of single nucleotide polymorphisms in the 3' region of the estrogen receptor 1 gene in normal and cryptorchid Miniature Dachshunds and Chihuahuas.

正常和隐睾小型腊肠犬和吉娃娃雌激素受体 1 基因 3 区域单核苷酸多态性分析。

• 发表时间: 2010
• 期刊: J Reprod Dev. 56(4)
• 作者: Pathirana IN;Tanaka K;Kawate N;Tsuji M;Kida K;Hatoya S;Inaba T;Tamada H.
• 通讯作者: Tamada H.

Establishment of canine induced pluripotent stem cells using retro virus and maintenance of their pluripotency in culture

使用逆转录病毒建立犬诱导多能干细胞并在培养物中维持其多能性

• 发表时间: 2011
• 作者: 今井裕;ら
• 通讯作者: ら

Canine oocyte maturation in culture : significance of estrogen and EGF receptor gene expression in cumulus cells.

培养中的犬卵母细胞成熟：卵丘细胞中雌激素和 EGF 受体基因表达的意义。

• 发表时间: 2009
• 期刊: Theriogenology 71
• 作者: Hatoya S;Sugiyama Y;Nishida H;Okuno T;Torii R;Sugiura K;Kida K;Kawate N;Tamada H;Inaba T.
• 通讯作者: Inaba T.

Canine oocyte maturation in culture : significance of estrogen and EGF receptor gene expression in cumulus cells

犬卵母细胞培养成熟：卵丘细胞中雌激素和EGF受体基因表达的意义

• 发表时间: 2009
• 期刊: Theriogenology 71
• 作者: Hatoya S;Sugiyama Y;Nishida H;Okuno T;Torii R;Sugiura K;Kida K;Kawate N;Tamada H;Inaba T
• 通讯作者: Inaba T

イヌES細胞の研究から再生獣医療に向けて

从犬ES细胞研究到再生兽医学

• 发表时间: 2012
• 作者: Mitsuya;S.;Kuwabara;J.;Ozaki,K.;Saeki,E.;Fujiwara;T. and Takabe,Tetsuko;稲葉俊夫
• 通讯作者: 稲葉俊夫