Central mechanism controlling the expression of estrous behavior and its manipulation in the dog.

控制狗发情行为表达及其操纵的中枢机制。

• 批准号: 10839011
• 负责人: INABA Toshio
• 金额: $ 2.5万
• 依托单位: Osaka Prefecture University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10839011/
• 关键词: Dog; Oestrus Induction; Gonadotrophin-Releasing Hormone; Hypothalamus; Anterior Pituitary; Estrogen Receptor mRNA; Estrogen; Luteinizing Hormone; 発情周期; 子宮; GnRHレセプター遺伝子; エストロジェンレセプター; プロジェステロン

Central mechanism controlling the expression of estrous behavior and stimulatory effects of a sustained release formulation of the gonadotrophin-releasing hormone agonist (GnRH-A) on reproductive function in the dog were examined, and the following results were obtained.1. An increase in the mean plasma levels of luteinizing hormone (LH) occurred from mid to late anestrus with the change in pulse patterns, followed by an increase in the mean plasma levels of estrogen and progesterone.2. The increase in GnRH release from hypothalamus could be responsible for the increasing secretion of LH which occurs during the progression of anestrus.3. Enhancement of hypothalamic estrogen receptor gene expression which may be regulated by estrogen might be one factor leading to the increased GnRH release in late anestrus.4. During the course of anestrus pituitary GnRH receptor gene expression increased while the pituitary responded accordingly.5. In proestrous bitches estrogen increased uterine sensitivity by increasing it's own receptor concentration, suggesting the preparation for estrus.6. A single injection of sustained-release formulation of GnRH-A in anestrous bitches resulted in an induced heat.These results suggest that, as a possible central mechanism controlling the expression of estrous behavior, the enhancement of hypothalamic estrogen receptor gene expression in late anestrus may lead to the increase in GnRH release, while pituitary responsiveness to thereby resulting in the resumption of ovarian and uterine activity with the return to estrus. They also indicate that microencapsulated LA can be useful in controlling the reproductive function in the dog.

研究了控制发情行为表达的中枢机制以及促性腺激素释放激素激动剂(GnRH-A)缓释制剂对犬生殖功能的刺激作用，得到以下结果： 1．动情中晚期，随着脉搏模式的变化，血浆黄体生成素（LH）平均水平升高，随后血浆雌激素和孕激素平均水平升高。2．下丘脑GnRH 释放的增加可能是在发情过程中LH 分泌增加的原因。3．受雌激素调节的下丘脑雌激素受体基因表达增强可能是导致动情晚期GnRH释放增加的因素之一。4．发情过程中垂体GnRH受体基因表达增加，垂体做出相应反应。 5．在发情前期的母犬中，雌激素通过增加其自身受体浓度来增加子宫敏感性，这表明为发情做准备。6．在不发情母犬中单次注射GnRH-A缓释制剂会导致发情。这些结果表明，作为控制发情行为表达的可能的中心机制，下丘脑雌激素受体基因表达的增强可能在不发情后期导致 GnRH 释放增加，同时垂体反应性从而导致卵巢和子宫活动恢复并恢复发情。他们还表明，微囊 LA 可用于控制狗的生殖功能。

项目成果

T.Inaba: "Reproduction in the dog : Control of estrus"InfoVets. 1・5. 4-8 (1998)

T.Inaba：“狗的繁殖：发情的控制”InfoVets 1・5（1998）。

Toshio Inaba: "Induction of fertile estrus in bitches using a sustained-relase formulation of a GnRH agonist(leuprolide acetate)" Theriogenology. 49・5. 975-982 (1998)

Toshio Inaba：“使用 GnRH 激动剂（醋酸亮丙瑞林）的持续释放制剂诱导母犬发情”，Theriogenology 49・5（1998）。

H. Tani, T. Inaba: "Increased LH pulse frequency and estrogen secretion associated with termination of anestrus followed By enhancement of uterine estrogen receptor gene expression in the beagle bitch"Theriogenology. 52・4. 593-607 (1999)

H. Tani, T. Inaba：“与不发情终止相关的 LH 脉冲频率和雌激素分泌增加，随后增强了比格犬母犬子宫雌激素受体基因的表达”Theriogenology 52·4 (1999)。

稲葉俊夫: "犬の臨床繁殖ー発情回帰の内分泌"InfoVets. 1・4. 45-49 (1998)

Toshio Inaba：“狗的临床繁殖 - 发情回归的内分泌学”InfoVets 1・4（1998）。

稲葉俊夫: "犬の臨床繁殖-発情の人為的調節"InfoVets. 1・5. 4-8 (1998)

Toshio Inaba：“狗的临床繁殖 - 发情的人工调节”InfoVets 1・5（1998）。