Development of fundamental technology for accurate RNAomics

开发精确RNA组学的基础技术

• 批准号: 20360374
• 负责人: FUKUSAKI Eiichiro
• 金额: $ 11.56万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20360374/
• 关键词: メタボロミクス; LC; MS; RNA; miRNA; ゼブラフィッシュ; ナノLC; 質量分析; プローブ

Fundamental technology including separation and mass spectrometry for miRNA measurement have been studied. Micro flow meter based separation system was optimized. LC system was established through optimization of elutant, matrix, column etc. De novo sequencing of miRNA (longer than 20 base) was achieved by means of iontrap-time of flight mass spectrometry based MS(N) analysis. Ion mobility based mass spectrometry was also performed to separate sequence isomers. In addition, RNA analogous MS probe was created.

研究了miRNA测量的分离和质谱等基础技术。对基于微流量计的分离系统进行了优化。通过优化洗脱液、基质、色谱柱等建立了液相色谱系统。通过基于离子阱飞行时间质谱的MS(N)分析实现了miRNA（大于20个碱基）的从头测序。还进行了基于离子淌度的质谱分析来分离序列异构体。此外，还创建了RNA类似MS探针。

项目成果

Metabolomics-based systematic predictionof yeast lifespan and its application for semirationalscreening of aging-related mutants

基于代谢组学的酵母寿命系统预测及其在衰老相关突变体半理性筛选中的应用

• 发表时间: 2010
• 期刊: Aging Cell 9(4)
• 作者: Yoshida;R.;T;Tamura;C.;Takaoka;K.;Harada;A.;Kobayashi;Y.;Mukai;E.;E.Fukusaki
• 通讯作者: E.Fukusaki

Demonstration of Three-Dimensional DNA Trapping by using Electric Force and Hydrodrag Force Field

利用电力和水拖力场进行三维 DNA 捕获演示

• 发表时间: 2011
• 期刊: to be published inJapanese Journal of Applied Physics
• 作者: Y.;Ukita;L.;Mouez;Y.;Tomizawa;Y.;Takamura
• 通讯作者: Takamura

Integrated metabolite and gene expression profiling revealing phytochrome

综合代谢物和基因表达谱揭示光敏色素

• 发表时间: 2008
• 期刊: A regulation of polyamine biosynthesisof Arabidopsis thaliana 59
• 作者: Jumtee;K. Bamba;T.
• 通讯作者: T.

Measurement of the binding affinity of selectedDNA aptamer by nonequilibrium capillaryelectrophresis

通过非平衡毛细管电泳测量所选 DNA 适体的结合亲和力

• 发表时间: 2010
• 作者: Yuuya Kasahara;Masayasu Kuwahara
• 通讯作者: Masayasu Kuwahara

安定同位体希釈率の経時変化情報による中央代謝ターンオーバー解析

使用稳定同位素随时间稀释的信息进行中央代谢周转分析

• 发表时间: 2010
• 作者: 中山泰宗;馬場健史;福崎英一郎
• 通讯作者: 福崎英一郎