Chloride concentration in cultured hippocampal neurons increases during long-term exposure to ammonia through enhanced expression of an anion exchanger

长期暴露于氨时，通过增强阴离子交换剂的表达，培养的海马神经元中的氯离子浓度增加

• 批准号: 10672163
• 负责人: HATTORI Naoki
• 金额: $ 2.05万
• 依托单位: Kansai Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10672163/
• 关键词: chloride ion; brain; ammonia; anion exchanger; protein kinase C; クロライドイオン; PKC; 海馬培養神経細胞; 陰イオン交換輸送体

Mechanisms of ammonia-induced increases in neuronal [ClィイD1=ィエD1]iA neurotoxic factor in hepatic encephalopathy, ammonia (2 mM, 48 hr), increased [ClィイD1-ィエD1]i in cultured rat hippocampal neurons. The increase was found to be due to enhanced expression of anion exchanger (AE3) mediated by protein kinase C activation.

氨诱导的神经元[CliiD1=ieD1]i增加的机制肝性脑病中的神经毒性因子，氨（2mM，48小时），培养的大鼠海马神经元中[CliiiD1-ieD1]i增加被发现是由于。蛋白激酶 C 激活介导的阴离子交换剂 (AE3) 表达增强。

项目成果

Hattori, N., Kitagawa, K., Higashida, T., Yagyu, K., Shimohama, S., Takaya, T., Perry, G., Smith, M. A. and Inagaki, C.: "ClィイD1-ィエD1-ATPase and NaィイD1+ィエD1, KィイD1+ィエD1-ATPase activities in Alzheimer's disease brains."Neurosci. Lett.. 254. 141-144 (1998)

服部，N.，北川，K.，东田，T.，柳生，K.，下滨，S.，高谷，T.，佩里，G.，史密斯，M.A.和稻垣，C.：“CliiD1-神经科学。莱特.. 254. 141-144 (1998)

Irie,T., Hara, M, Yasukura, T., Minamino, M., Omori, K., Matsuda, H., Inoue, K. and Inagaki, C.: "Chloride concentration in cultured hippocampal neurons increases during long-term exposure to ammonia through expression of anion exchanger."Brain Res. 806.

Irie,T.、Hara, M、Yasukura, T.、Minamino, M.、Omori, K.、Matsuda, H.、Inoue, K. 和 Inagaki, C.：“培养的海马神经元中的氯化物浓度在长期

Zeng, X.-T et al.: "Antiserum against Cl^- pump complex recognized 51 kDa protein, a possible catalytic unit in the rat brain"Neurosci. Lett. 258. 85-88 (1998)

Zeng, X.-T 等人：“抗 Cl^-泵复合物的抗血清识别出 51 kDa 蛋白质，这是大鼠大脑中可能的催化单元”Neurosci。

Hattori, N., Kaori Kitagawa and Chiyoko Inagaki: "Human lymphocytes express hGH-N gene transcripts of 22kDa, 20kDa and minor forms of GH, but not hGH-V gene."Eur. J. Endocrinol. 141. 413-418 (1999)

Hattori, N.、Kaori Kitakawa 和 Chiyoko Inagaki：“人类淋巴细胞表达 22kDa、20kDa 的 hGH-N 基因转录物和少量 GH，但不表达 hGH-V 基因。”Eur。

Hattori, N. and Inagaki, C.: "Immunological aspects of human growth hormone and prolactin."Domestic Animal Endocrynology. 15. 371-375 (1998)

Hattori, N. 和 Inagaki, C.：“人类生长激素和催乳素的免疫学方面。”国内动物内分泌学。