Study on the flower color change of morning glory by change of vacuolar pH

改变液泡pH值对牵牛花花色变化的研究

• 批准号: 10660115
• 负责人: YOSHIDA Kumi
• 金额: $ 2.11万
• 依托单位: Nagoya University (2000)Sugiyama Jogakuen University (1998-1999)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10660115/
• 关键词: Ipomoea tricolor; vacuolar pH; microelectrode; protoplast; vacuolar K^+; flower color change; vacuolar membrane; Pharbitis nil; 液胞カリウムイオン濃度; 細胞内微小pH電極; V-ATPase; V-PPase; 細胞内微小K電極; 免疫電子顕微鏡観察

The flower color pigment anthocyanin changes its color continuously in vitro by the change of pH.One of the the causes of this the color change of the flower variously will be a difference of vacuole pH in which the pigment exists intracellular. This study was done for the purpose of quantitatively clarifying the correlation of vacuole pH and flower color by developing and establishing direct measurement method in this study of petal vacuole pH by the intracellular micro-electrode method.Electrophysiology loboratory-table for the ion concentration measurement in the petal vacuole by the micro-electrode method was constructed. It also carried out besides besides pH electrode preparation of intracellular minute potassium responsibility electrode which filled the potassium ionophore.The vacuolar pH of Blue morning glory (Ipomoea tricolor, cv.Heavenly blue), red Japan morning glory (Pharbitis nil cv.Scarlet O'hara) petals were measured. Though in the red buds petals vacuole pH was about 6.6, the pH value of open flower was 7.6 The vacuolar pH of the red morning glory was about 6.1, and it was proven to be considerably lower than 7.6 of the sky-blue morning glory.Potassium ion concentration of the purple petal cell vacuole of the morning glory was 70 mM.The ivacuolar potassium ion concentration of blue opened flower was 38 mM.The relationship between flower color and ion concentration in the vacuole has much ineterst and the study are under progress.

花朵颜色色素花青素在体外随着pH值的变化而不断改变颜色。花朵颜色发生各种变化的原因之一是色素存在于细胞内的液泡pH值的差异。本研究旨在通过开发和建立细胞内微电极法直接测量花瓣液泡pH值的方法，定量阐明液泡pH值与花色的相关性。 离子浓度测量的电生理实验表采用微电极法构建花瓣液泡。 [0120] 除了填充钾离子载体的细胞内微小钾责任电极之外，还进行了pH电极制备。蓝色牵牛花(Ipomoea tricolor, cv.Heavenly blue)、红色日本牵牛花(Pharbitis nil cv.Scarlet O')的液泡pH hara）花瓣被测量。虽然红色花蕾花瓣液泡pH值为6.6左右，但开放花的pH值为7.6。红色牵牛花液泡pH值为6.1左右，事实证明大大低于天蓝色牵牛花的7.6。牵牛花紫色花瓣细胞液泡钾离子浓度为70 mM。蓝色开放花液泡钾离子浓度为38 mM.花的颜色与液泡中离子浓度的关系尚不明确，研究正在进行中。

项目成果

Kondo, T., Oyama, K., Yoshida, K.: "Chiral Molecular Recognition on Formation of a Metalloanthocyanin : A Supramolecular Metal Complex Pigment from Blue Flower of Salvia patens."Angew.Chem.Int.Ed.. 40 (In press). (2001)

Kondo, T.、Oyama, K.、Yoshida, K.：“金属花青素形成的手性分子识别：来自丹参蓝花的超分子金属复合颜料。”Angew.Chem.Int.Ed. 40（In

Yoshida,K. et al: "Contribution of Each Caffeoyl Residue of the Pigment Molecule of Gentiodelphin to Blue Color Development."Phytochemistry. 54. 85-92 (2000)

吉田，K.

Yoshida,K. et al: "Contribution of Each Caffeoyl Residue of the Pigment Molecule of Gentiodelphin to Blue Color Development."Phytochemistry. 54. 85-92 (2000)

吉田，K.

Yoshida,K.et al.: "Structures of two diacylated anthocyanins from Petunia hybrida cv. surfinia violet mini." Phytochemistry. 47. 1409-1416 (1998)

Yoshida,K.et al.：“来自矮牵牛品种 surfinia violet mini 的两种二酰化花青素的结构。”

Yoshida, K., Toyama, Y., Kameda, K., Kondo, T.: "Contribution of Each Caffeoyl Residue of the Pigment Molecule of Gentiodelphin to Blue Color Development."Phytochamistry. 54. 85-92 (2000)

Yoshida, K.、Toyama, Y.、Kameda, K.、Kondo, T.：“龙胆色素分子的每个咖啡酰残基对蓝色发展的贡献。”植物化学。