Selection of malaria protective sera useful for novel vaccine candidate discovery

选择有助于新型候选疫苗发现的疟疾保护血清

• 批准号: 19406009
• 负责人: TSUBOI Takafumi
• 金额: $ 10.73万
• 依托单位: Ehime University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19406009/
• 关键词: 感染症; マラリア; ワクチン; ゲノム; タイ; バイオテクノロジー; 国際研究者交流

The individuals live in malaria endemic area who repeatedly exposed to the malaria parasite gradually develop immunity to the clinical manifestations of infection. This resistance to clinical disease is partly mediated by antibodies against the asexual blood stages of the malaria parasite. To identify such individuals harboring protective antibodies we have screened the human serum specimen useful for the future vaccine candidate discovery. After three-years surveillance in the endemic village in western Thailand, we have successfully obtained both asymptomatic sera and symptomatic patient sera as negative controls.

这些个体生活在疟疾流行地区，他们反复暴露于疟原虫，逐渐对感染的临床表现产生免疫力。这种对临床疾病的抵抗力部分是由针对疟原虫无性血统的抗体介导的。为了识别具有保护性抗体的这样的人，我们已经筛选了人类血清标本，可用于未来的疫苗候选发现。在泰国西部地方性村庄进行了三年的监视之后，我们成功获得了无症状的血清和有症状的患者血清作为阴性对照。

项目成果

Transmission-blocking activity of DNA vaccine encoding Plasmodium vivax gametocyte protein Pvs230

编码间日疟原虫配子体蛋白 Pvs230 的 DNA 疫苗的传播阻断活性

• 发表时间: 2007
• 作者: J. Okuda;T. Ramamruthy;S. Yamasaki;Tachibana M.
• 通讯作者: Tachibana M.

Novel sporozoite antigen discoverly of Plasmodium falciparum screened using human antisera.

使用人抗血清筛选恶性疟原虫的新子孢子抗原。

• 发表时间: 2007
• 作者: 松本昂;山田健太郎;高田賢蔵;西園晃;山崎伸二;Tsuboi T.
• 通讯作者: Tsuboi T.

Plasmodium falciparum rhoptry neck protein (PfRON2) expressed at both erythrocytic and pre-erythrocytic invasive parasites.

恶性疟原虫棒状颈部蛋白 (PfRON2) 在红细胞和红细胞前侵袭性寄生虫中表达。

• 发表时间: 2007
• 作者: J. Okuda;T. Ramamurthy;S. Yamasaki;Cao J.
• 通讯作者: Cao J.

The Plasmodium falciparum RhopH2 promoter and first 24 amino acids are sufficient to target proteins to the rhoptries

• DOI: 10.1016/j.parint.2006.11.001
• 发表时间: 2007-03-01
• 期刊: PARASITOLOGY INTERNATIONAL
• 影响因子: 1.9
• 作者: Ghoneim, Ahmed;Kaneko, Osamu;Torii, Motomi
• 通讯作者: Torii, Motomi

Novel sporozoite antigen discovery of Plasmodium falciparum screened using human immunesera

使用人免疫血清筛选恶性疟原虫的新子孢子抗原的发现

• 发表时间: 2007
• 作者: Kaneko;O;Han ET;Palacpac NM;Kaneko O;Yano K;Tsuboi T
• 通讯作者: Tsuboi T