Identification of RBC receptors against malaria parasite molecules in the merozoite apical organelle

裂殖子顶端细胞器中针对疟原虫分子的红细胞受体的鉴定

• 批准号: 21249028
• 负责人: TSUBOI Takafumi
• 金额: $ 30.87万
• 依托单位: Ehime University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21249028/
• 关键词: マラリア; レセプター; コムギ胚芽無細胞タンパク質合成系; ゲノム; 感染症; バイオテクノロジー

In order to identify novel molecules localized in the apical organelles of Plasmodium merozoite, we have expressed 193 putative merozoite specific molecules and produced antibodies against each recombinant protein. Among them, 29 were localized at the apical end of the merozoite suggesting novel apical organellar proteins. We then characterized the RBC binding activity and the RBC receptor phenotype against one of the novel micronemal molecules, GAMA. Furthermore, we have constructed erythrocyte protein array including 500 of molecules, and identified an erythrocyte membrane protein which serves as a receptor against erythrocyte-binding rhoptry protein.

为了鉴定位于疟原虫裂殖子顶端细胞器中的新分子，我们表达了 193 种推定的裂殖子特异性分子，并产生了针对每种重组蛋白的抗体。其中，29 个位于裂殖子的顶端，表明存在新的顶端细胞器蛋白。然后，我们对一种新型微线体分子 GAMA 的红细胞结合活性和红细胞受体表型进行了表征。此外，我们构建了包含500个分子的红细胞蛋白阵列，并鉴定了一种红细胞膜蛋白，其作为红细胞结合棒状体蛋白的受体。

项目成果

Two post-genome approaches for the discovery of novel malaria blood-stage vaccine candidates using wheat germ cell-free system

使用小麦胚芽无细胞系统发现新型疟疾血液阶段候选疫苗的两种后基因组方法

• 发表时间: 2012
• 作者: Chen J;Neu J;Miyata M;Oster G;Tsuboi T
• 通讯作者: Tsuboi T

Sexual stage parasites and transmission-blocking antibodies

有性阶段寄生虫和传播阻断抗体

• 发表时间: 2010
• 作者: 馬場崇;三村純正;嶋雄一;中村直人;大脇亜希子;大島基彦;原田信広;山本雅之;藤井義明;諸橋憲一郎;Tsuboi T
• 通讯作者: Tsuboi T

Discovery of GAMA, a Plasmodium falciparum Merozoite Micronemal Protein, as a Novel Blood-Stage Vaccine Candidate Antigen

• DOI: 10.1128/iai.05412-11
• 发表时间: 2011-11-01
• 期刊: INFECTION AND IMMUNITY
• 影响因子: 3.1
• 作者: Arumugam, Thangavelu U.;Takeo, Satoru;Tsuboi, Takafumi
• 通讯作者: Tsuboi, Takafumi

Antibodies against a Plasmodium falciparum antigen PfMSPDBL1 inhibit merozoite invasion into human erythrocytes

• DOI: 10.1016/j.vaccine.2012.01.010
• 发表时间: 2012-03-02
• 期刊: VACCINE
• 影响因子: 5.5
• 作者: Sakamoto, Hirokazu;Takeo, Satoru;Tsuboi, Takafumi
• 通讯作者: Tsuboi, Takafumi

Rhoptry neck protein RON2 forms a complex with microneme protein AMA1 in Plasmodium falciparum merozoites

恶性疟原虫裂殖子中棒状体颈蛋白 RON2 与微线体蛋白 AMA1 形成复合物

• DOI: 10.1016/j.parint.2008.09.005
• 发表时间: 2009-03-01
• 期刊: PARASITOLOGY INTERNATIONAL
• 影响因子: 1.9
• 作者: Cao, Jun;Kaneko, Osamu;Torii, Motomi
• 通讯作者: Torii, Motomi