Molecular mechanisms underlying the membrane sorting of bacterial lipoproteins

细菌脂蛋白膜分选的分子机制

• 批准号: 19380046
• 负责人: TOKUDA Hajime
• 金额: $ 12.06万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19380046/
• 关键词: 膜局在化; ABCトランスポーター; 分子シャペロン; ペリプラズム; リポ蛋白質; 光架橋反応; LolA; LolB; LolCDE; 選別シグナル; 大腸菌

Lipoproteins possessing the lipid-attached cysteine residue at the N-terminus are widely distributed among bacteria and anchored to membranes through the N-terminal lipids. Lipoproteins are involved in various functions present in cell surfaces and localized in either the inner or outer membrane of Gram-negative bacteria depending on their sorting signals. We have reveled that five Lol factors are involved in the membrane sorting of lipoproteins. The mechanisms underlying the specific membrane sorting of lipoproteins by Lol proteins are examined in this study.

N 末端具有脂质附着的半胱氨酸残基的脂蛋白广泛分布在细菌中，并通过 N 末端脂质锚定在细胞膜上。脂蛋白参与细胞表面存在的各种功能，并根据其分选信号定位在革兰氏阴性细菌的内膜或外膜中。我们发现五个 Lol 因子参与脂蛋白的膜分选。本研究探讨了 Lol 蛋白对脂蛋白进行特异性膜分选的机制。

项目成果

Complete reconstitution of an ATP-binding cassette transporter LolCDE complex from separately isolated subunits

• DOI: 10.1111/j.1742-4658.2007.05832.x
• 发表时间: 2007-07-01
• 期刊: FEBS JOURNAL
• 影响因子: 5.4
• 作者: Kanamaru, Kyoko;Taniguchi, Naohiro;Tokuda, Hajime
• 通讯作者: Tokuda, Hajime

Backbone resonance assignment for the outer membrane lipoprotein receptor LolB from Escherichia coli

大肠杆菌外膜脂蛋白受体 LolB 的骨架共振分配

• 发表时间: 2007
• 期刊: Biomol. NMR Assign. 1
• 作者: Tateishi;Y.;Ariyoshi;H.;Igarashi;R.;Hara;H.;Mizuguchi;K.;Seto;A.;Nakai;A.;Kolcubo;T.;Tochio;H. and Shirakawa;M;杉田有治;Masayuki Oda;H. Takahashi;Shingo Nakada
• 通讯作者: Shingo Nakada

Introduction of a lethal redox switch that controls the opening and closing of the hydrophobic cavity in LolA.

引入致命的氧化还原开关，控制 LolA 中疏水腔的打开和关闭。

• 发表时间: 2008
• 期刊: J.Biol.Chem. 283
• 作者: Watanabe;S.;Oguchi;Y.;Takeda;K.;Miki;K.;Tokuda;H.
• 通讯作者: H.

Large-scale preparation of the homogeneous LolA-lipoprotein complex and efficient in vitro transfer of lipoproteins to the outer membrane in a LolB-deperldent manner

大规模制备同质 LolA-脂蛋白复合物并以 LolB 依赖性方式在体外将脂蛋白有效转移到外膜

• 发表时间: 2007
• 期刊: Protein Sci 16
• 作者: Watanabe;S.
• 通讯作者: S.

A novel complete reconstitution system for membrane integration of the simplest membrane protein

• DOI: 10.1016/j.bbrc.2010.03.061
• 发表时间: 2010-04-09
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Nishiyama, Ken-ichi;Maeda, Masahide;Tokuda, Hajime
• 通讯作者: Tokuda, Hajime