Protein translocation across the cytoplasmic membrane of bacteria

蛋白质跨细菌细胞质膜易位

• 批准号: 07308069
• 负责人: TOKUDA Hajime
• 金额: $ 6.14万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07308069/
• 关键词: Protein translocation; Prokaryote; Reconstitution; SecY; SecE; SecG; SecA

1. SecG was found to interact with SecA and facilitate the membrane insertion-deinsertion cycle of SecA.2. Mode of interaction of SecY-SecE-SecG complex was revealed.3. SecA was found to be involved in the import of cytochrome f into thylakoid.4. SecY and SecE were reconstituted into a planar lipid bilayr membrane and found to form a ion-conducting channel.5. Step-wise translocation of precursor protein was found to be caused by a hydrophobic region of the precursor.6. Structure and function of Bacillus subtilis SRP homologue were revealed.7. Highly active protein translocation system was reconstituted into proteoliposomes.

1. 发现 SecG 与 SecA 相互作用并促进 SecA.2 的膜插入-脱插入循环。揭示了SecY-SecE-SecG复合物的相互作用模式。 3． SecA被发现参与细胞色素f向类囊体的输入。 4．将SecY和SecE重构为平面脂质双层膜，并发现其形成离子传导通道。 5．发现前体蛋白的逐步易位是由前体蛋白的疏水区域引起的。6．揭示了枯草芽孢杆菌SRP同源物的结构和功能。 7．高活性的蛋白质易位系统被重构为脂蛋白体。

项目成果

Oguro,A.: "The effect of expression of srb,encoding a homologue of mammalian SRP receptor a-subunit,on cell growth and protein translocation in Bacillus subtilis and srb operon structure." Gene. 172. 17-24 (1996)

Oguro,A.：“编码哺乳动物 SRP 受体 a 亚基同源物的 srb 表达对枯草芽孢杆菌和 srb 操纵子结构中细胞生长和蛋白质易位的影响。”

Homma, T., Yoshihisa, T., Kihara, A., Akiyama, Y., and Ito, K.: "Intracellular stability of a fragment of beta-galactosidase : effects of amino-terminally fused polypeptides." Biochem. Biophys. Res. Commun.215. 452-458 (1995)

Homma, T.、Yoshihisa, T.、Kihara, A.、Akiyama, Y. 和 Ito, K.：“β-半乳糖苷酶片段的细胞内稳定性：氨基末端融合多肽的影响”。

Nishiyama,K.: "Inversion of the membrane topology of SecG coupled with SecA-dependent preprotein translocation." Cell. 85. 71-81 (1996)

Nishiyama,K.：“SecG 膜拓扑结构的反转与 SecA 依赖性前蛋白易位相结合。”

Uchida,K.: "Stepwise Movement of Preproteins in the Process of Translocation across the Cytoplasmic Membrance of Escherichia coli." J.Biol.Chem.270. 30862-30868 (1995)

Uchida,K.：“前蛋白在大肠杆菌细胞质膜易位过程中的逐步运动。”

Sato,K.: "Short hydrophobic segments in the mature domain of proOmpA determine its stepwise movement during translocation across the cytoplasmic membrane of Escherichia coli" J.Biol.Chem.272(in press). (1997)

Sato,K.：“proOmpA 成熟结构域中的短疏水片段决定了其在大肠杆菌细胞质膜易位过程中的逐步运动”J.Biol.Chem.272（出版中）。