Sorting and membrane localization of E.coli lipoproteins

大肠杆菌脂蛋白的分选和膜定位

• 批准号: 15208009
• 负责人: TOKUDA Hajime
• 金额: $ 28.12万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15208009/
• 关键词: protein transport; molecular chaperone; reconstitution; Escherichia coli; ABC transporter; lipoprotein; membrane localization; sorting signal

E.coli lipoproteins are sorted to the outer membrane depending on the amino acid residue located next to the amino-terminal lipid-attached cysteine. The sorting is mediated by the Lol system composed of five Lol proteins. The lipoprotein transfer reaction from LolA to LolB is unidirectional and requires no energy. This reaction was found to depend on the strength of hydrophobic interaction between lipoproteins and LolA or LolB. If the LolA-lipoprotein interaction is too strong, the transfer of lipoprotein to LolB is inhibited. Opening and closing of the hydrophobic cavity of LolA was found to be critical for the LolA function. Finally, the region of LolD important for the interaction with membrane subunits LolC and LolE was determined.

根据氨基末端脂质附着的半胱氨酸旁边的氨基酸残基，将大肠杆菌脂蛋白分选到外膜。分选是由五个 Lol 蛋白组成的 Lol 系统介导的。从 LolA 到 LolB 的脂蛋白转移反应是单向的，不需要能量。发现该反应取决于脂蛋白与 LolA 或 LolB 之间疏水相互作用的强度。如果LolA-脂蛋白相互作用太强，脂蛋白向LolB的转移就会受到抑制。研究发现 LolA 疏水腔的打开和关闭对于 LolA 功能至关重要。最后，确定了对于与膜亚基 LolC 和 LolE 相互作用重要的 LolD 区域。

项目成果

蛋白質輸送とエンベロープの形成(原核細胞)

蛋白质运输和包膜形成（原核细胞）

• 发表时间: 2004
• 期刊: 蛋白質・核酸・酵素 49
• 作者: Inubushi;K.;(Oslan Jumadi);徳田元
• 通讯作者: 徳田元

Mechanisms underlying energy-independent transfer of lipoproteins from LolA to LolB, which have similar unclosed β-barrel structures.

脂蛋白从 LolA 到 LolB 的能量依赖性转移的机制，LolB 具有类似的未封闭 β 桶结构。

• 发表时间: 2005
• 期刊: J. Biol. Chem. 280
• 作者: Taniguchi;N.
• 通讯作者: N.

argeted mutagenesis of five conserved tryptophan residues of LolB involved in membrane localization of Escherichia coli lipoproteins.

对参与大肠杆菌脂蛋白膜定位的 LolB 的五个保守色氨酸残基进行定向诱变。

• 发表时间: 2004
• 期刊: Biochim. Biophys. Ress. Commun. 323
• 作者: Wada;R.
• 通讯作者: R.

A practical phasing procedure of MAD method without the aid of XAFS measurements : successful solution in the structure determination of the outer membrane lipoprotein carrier LolA.

不借助 XAFS 测量的 MAD 方法的实用定相程序：成功解决了外膜脂蛋白载体 LolA 结构测定的问题。

• 发表时间: 2003
• 期刊: Acta Cryst. D59
• 作者: Takeda;K.
• 通讯作者: K.

Effects of lipoprotein overproduction on the induction of DegP (HtrA) involved in the quality control of Escherichia coli periplasm.

脂蛋白过量产生对参与大肠杆菌周质质量控制的 DegP (HtrA) 诱导的影响。

• 发表时间: 2004
• 期刊: J.Biol.Chem. 279
• 作者: Miyadai;H.
• 通讯作者: H.