Elucidation of mechanism on "French Paradox" toward prevention of lifestyle-related diseases

阐明“法国悖论”预防生活习惯病的机制

• 批准号: 19300250
• 负责人: INOUE Hiroyasu
• 金额: $ 12.56万
• 依托单位: Nara Women's University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19300250/
• 关键词: PPAR; シクロオキシゲナーゼ; 生活習慣病; レスベラトロール; 精油成分; 食品機能成分; 核内受容体; フレンチパラドックス; 発現制御; 食品機能

Dairy oral intake of resveratrol, a phytoalexin contained in red wine, reduces plasma triglyceride concentration, and upregulates hepatic expression of PPAR-α responsive genes in the wild-type but not PPARα knockout mice. Moreover, long-term intake of 0.02% resveratrol with high fat diet prevents body weight gain observed after 46 weeks and accumulation of abdominal white adipose tissue, and improves survival of mice. These findings will account for a novel mechanism that may afford beneficial effects of preventing lifestyle-related diseases.Several natural chemicals such as resveratrol have been identified as suppressors of COX-2 expression and activators of PPARs. These two properties targeted to COX-2 and PPARs will be useful in evaluating functional food components against lifestyle-related diseases. In this context, we evaluated oils from various plants in cell-based transfection assays, and found that six essential oils from thyme, rose, clove, fennel, eucalyptus, and bergamot, have these properties. From thyme oil, we identified carvacrol as a major component of the activator of PPARα and γ and suppressor of COX-2 expression, and PPARγ-dependent suppression of COX-2 promoter activity was observed in response to carvacrol treatment. These results will be important in understanding the anti-lifestyle-related disease properties of essential oils.

白藜芦醇的乳制品摄入是红酒中包含的植物毒素，可降低野生型中的血浆甘油三酸酯浓度PPAR-α反应基因，而不是PPARα基因敲除小鼠。观察到46周后观察到的，腹部白脂肪组织的积累将有可能带来与生活方式相关的疾病的影响。 COX-2在基于细胞的transe液中有助于针对生活方式相关疾病的评估食品成分。 PPARα，γ和γ的活化剂的主要成分以及Cox-2启动子活性的PPARγ依赖性的抑制作用是对Carvacrol处理的响应。精油的疾病特性。

项目成果

Transcriptional regulation of the COX-2 expression by nitric oxide in colon cancer cell lines.

结肠癌细胞系中一氧化氮对 COX-2 表达的转录调节。

• 发表时间: 2008
• 期刊: Oncol Rep. 19
• 作者: Liu Q;Inoue H;Mahendran R
• 通讯作者: Mahendran R

誘導型シクロオキシゲナーゼ発現抑制を指標にした植物油の機能性評価

以抑制诱导型环氧合酶表达为指标的植物油功能评价

• 发表时间: 2008
• 期刊: 日本家政学会誌 59
• 作者: 堀田真理子;中田理恵子;井上裕康
• 通讯作者: 井上裕康

Activation of PPAR by resveratrol and other polyphenols

白藜芦醇和其他多酚激活 PPAR

• 发表时间: 2007
• 作者: 塚本朋子;(井上 裕康);井上裕康
• 通讯作者: 井上裕康

レスベラトロール4量体バチカノールCによる核内受容体PPAR a およびβ/δの活性化

白藜芦醇四聚体 Vaticanol C 激活核受体 PPAR a 和 β/δ

• 发表时间: 2008
• 作者: 塚本朋子;中田理恵子;勝川路子;赤尾幸博;野澤義則;三島敏;井上裕康;井上 裕康
• 通讯作者: 井上 裕康

Vaticanol C, a resveratrol tetramer

Vaticanol C，白藜芦醇四聚体

• 发表时间: 2010
• 期刊: activates PPARαand PPARβ/δin vitro and in vivo Nutrition & Metabolism 7
• 作者: Tsukamoto T.;Nakata R.;Tamura E.;Kosuge Y.;Kariya A.;Katsukawa M.;Mishima S.;Itoh;T.;Iinuma M.;Akao Y.;NozawaY.;Arai Y.;Namura S.;Inoue H.
• 通讯作者: Inoue H.