Function of mouse lectin receptors

小鼠凝集素受体的功能

基本信息

批准号：
18390121
负责人：
INABA Kayo
金额：
$ 10.64万
依托单位：
Kyoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

In this study, we aimed to elucidate role of mouse C-type lectins, SIGNR3 and DCIR1. SIGNR3 is a homologue of mouse DC-SIGN and recognizes zymosan and C. albicans. We established new monoclonals antibody specific for SGINR3 and examined the distribution of SIGNR3^+ cells in situ. They were shown to localize in dermis, subcutaneous lymph nodes, and circulating blood. There were two types of SIGNR3^+ cells in the dermis and node ; one was CD11c^+ dendritic cells and the other macrophages. In lymph nodes, they were distributed in interfollicular regions, near HEV in paracortex and medulla. SIGNR3 is expressed only in CD115^+ Ly6C^<int-low> monocytes in the blood. During inflammatory response, a large increase in SIGNR3^+ cells is observed in draining nodes, particularly around HEV. These results imply the possibility that SIGNR3^+ monocytes are recruited into inflamed tissue. This study aimed to examine this possibility.On the other hand, expression of DCIR1, which is characterized by the presence of ITIM in its cytoplasmic portion, was detected not only dendritic cells, but also B cells, neutrophil, monocytes and macrophages by Western blot and flowcytometry. Interestingly, DCIR1 molecules were present mainly in cytoplasm in the steady state and the surface expression was up-regulated by the stimulation with inflammatory cytokines and TLR ligands without de novo synthesis. In addition, granulocytes recruited into inflammatory site expressed abundant DCIR1 on cell surface. Similar phenomenon was also observed in HL60 cells after stimulation with DMSO that induces differentiation to neutrophil. DCIR1 shared EPN motif with SIGNR3 and was found to bind to zymosan, heat-killed and live C. albicans. Upon cross-linking with specific antibody, DCIR1 was phosphorylated and associated with SHP-1 and SHP-2. Therefore, DCIR1-mediated signal may be involved in the regulation of inflammatory response.

在这项研究中，我们旨在阐明小鼠C型凝集素，SignR3和DCIR1的作用。 SIGNR3是小鼠DC-sign的同源物，并识别Zymosan和C. bilicans。我们建立了针对SGINR3的新单克隆抗体，并检查了Signr3^+细胞原位的分布。它们被证明在真皮，皮下淋巴结和循环血液中定位。真皮和节点中有两种类型的Signr3^+细胞。一个是CD11C^+树突状细胞和其他巨噬细胞。在淋巴结中，它们分布在副羊角和髓质的HEV附近的室内区域。 SIGNR3仅在血液中的CD115^+ ly6c^<int-low>单核细胞中表达。在炎症反应过程中，在排水节点，尤其是在HEV周围，观察到Signr3^+细胞的大幅增加。这些结果暗示了Signr3^+单核细胞被募集到发炎的组织中的可能性。这项研究旨在检查这种可能性。另一方面，DCIR1的表达不仅检测到具有ITIM在其细胞质部分中的ITIM的特征，不仅被检测到树突状细胞，还检测到B细胞，中性粒细胞，单细胞和巨噬细胞。和流循环仪。有趣的是，DCIR1分子主要存在于稳态中的细胞质中，并且表面表达通过炎性细胞因子和没有从头合成的炎症细胞因子和TLR配体的刺激上调。此外，募集到炎症部位的粒细胞在细胞表面表达了丰富的DCIR1。用DMSO刺激诱导中性粒细胞的DMSO刺激后，在HL60细胞中也观察到了类似的现象。 DCIR1与SIGNR3共享EPN基序，发现与Zymosan结合，热杀死和活白色念珠菌。与特异性抗体交联后，DCIR1被磷酸化并与SHP-1和SHP-2相关。因此，DCIR1介导的信号可能参与炎症反应的调节。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Analysis of cellular cytokine production in response to nanomaterials and asbestos

纳米材料和石棉响应的细胞细胞因子产生分析

DOI：
发表时间：
2007
期刊：
影响因子：
0
作者：
Kazuhiko;Takahara
通讯作者：
Takahara

Repetitive injection of dendritic cells pulsed with α- galactosylceramide effectively induce tolerance in an antigen- dependent and independent manner

重复注射α-半乳糖神经酰胺脉冲的树突状细胞以抗原依赖性和非依赖性方式有效诱导耐受

DOI：
发表时间：
2007
期刊：
影响因子：
0
作者：
Akiko;Hayuka
通讯作者：
Hayuka

Role of SIGNR1 in the recognition and response to pathogenic fungus

SIGNR1 在病原真菌识别和反应中的作用

DOI：
发表时间：
2007
期刊：
影响因子：
0
作者：
Shin DM;Yang CS;Yuk JM;Lee JY;Kim KH;Shin SJ;Takahara K;Lee SJ;Jo EK.;Shin DM;Koichi Tanaka;Cheolho Cheong;Kenji Kabashima;Sayuri Yamazaki;高原和彦;長岡孝治;長岡孝治;高原和彦;高原和彦;高原和彦;高原和彦;稲葉カヨ;稲葉カョ;吉田洋子;長岡孝治;長岡孝治;吉田洋子;高原和彦;高原和彦
通讯作者：
高原和彦

Stable mixed chimerism is induced by NK cell depletion in MHC-mismached, T cell-depleted, nonmyeloablative bone marrow transplantation

MHC 错配、T 细胞耗尽、非清髓性骨髓移植中 NK 细胞耗尽可诱导稳定的混合嵌合状态