Study For Functional Features of The Dendritic cell

树突状细胞功能特征的研究

• 批准号: 06044124
• 负责人: INABA Kayo
• 金额: $ 4.16万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06044124/
• 关键词: Dendritic cells; Antigen presentation; Cell differentiation,; Functional maturation; T cell activation; 単クローン抗体

Dendritic cells belong to the myeloid linearge of hematopoietic cells and are the most potent in T cell activation for in the initiation of immune responses. The aim of this project is futher clarification of the functional role of dendritic cells in vivo and in vitro in relation to the cellular development and maturation.1) The NLDC-145 mAb recognizes a 205 kDa integral membrane protein which is expressed at high levels on murine dendritic cells and thymic epithelial cells (DEC-205). We reexamined cell specificity by mean of cytofluorographic and histological approaches and potential function of this molecule. DEC-205 can also be detected on many other subsets of leukocytes, particularly B cells. Although we have been unable to inhibit the alloreactivity that is induced by dendritic cells in vitro and in vivo, DEC-205 is shown to be rapidly internalized via coated pits and vesicles, and delivered to a multivesicular endosomal compartment resembling MIIC.A rabbit anti-DEC-205 IgG is pr … More esented by DCs to rabbit IgG-specific T cell hybridomas 100 times more efficiently than normal rabbit IgG.Considering that DEC-205 is a receptor with 10 C-type lectin domains, this molecule may be a novel endocytic receptor to capture glycoprotein antigens from extracellular space to specialized antigen processing compartment.2) Age-related immunological dysfunction is demonstrated to be ascribable to the decreased T-cell stimulating activity of dendritic cells and B cells in SAMP1 mice. This change is primarily due to the reduced expression of class II and CD54, but not CD80 and CD86.3) The HIV-1 promoter has two key transcriptional controls : NF-kB sites and Sp1 sites. Therefore, we looked for NF-kB and Sp1 factors in different cell types from human and mouse, using many biochemical and electromobility shift assays. Dendritic cells expressed high levels of all known NF-kB,but Sp1 was lacking. Quiescent T cells lacked active NF-kB but expressed Spl.By inducing heterologous dendritic-T cell syncytia, HIV brought together high levels of the essential factors for the viral promoter. This phenomenon would lead to chronic replication of HIV-1 without ostensible immune stimulation. Less

造血细胞的骨髓线性和t -cell激活中最有效的骨髓线性。细胞和胸腺上皮细胞（DEC-205）。 DEC-205由树突状细胞在体外和体内诱导，通过涂层坑和囊泡迅速内化了DEC-205，并传递到类似于Miic.a t anti-Deco 205 IgG的多个内体内体隔室，由DCS pr。对于兔IgG特异性T细胞杂交瘤比正常兔IgG高100倍。考虑到DEC-205是一种具有10 C型凝集素域抗原的受体是从细胞外空间到专业加工型的受体。证明d是SAMP1小鼠的树突状细胞和B细胞的T细胞刺激的降低。 KB位点和SP1位置。 dendrititic-t细胞合成剂的高水平启动子的基本因素将导致HIV-1的慢性复制，而没有过度启动的免疫刺激

项目成果

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T.Ichiki 等人：“小鼠血管紧张素 II 2 型受体 cDNA 和基因组 DNA 的克隆。”

Witmer-Pack,M.D.: "Tissue distribution of the DEC-205 protein that is detected by the monoclonal antibody NLDC-145.II.Expression in situ in lymphoid and nonlymphoid tissues." Cell.Immunol.163. 157-162 (1995)

Witmer-Pack，医学博士：“通过单克隆抗体 NLDC-145.II 检测到的 DEC-205 蛋白的组织分布。淋巴和非淋巴组织中的原位表达。”

H.Itoh et al.: "Antagonism between vascular renin-angiotersin and natriuretic peptide systems in vascular remodeling" Blood Pressure. 3. 49-53 (1994)

H.Itoh 等人：“血管重塑中血管肾素-血管紧张素和利尿钠肽系统之间的拮抗作用”血压。

G.Shirakami et al.: "Effects of anesthesia and surgery on plasma endothelin levels." Anesth.Analg.80. 449-453 (1995)

G.Shirakami 等人：“麻醉和手术对血浆内皮素水平的影响。”

Haruna,H: "Abnormalities of B cells and dendritic cells in SAMP1 mice." Eur.J.Immunol.25. 1319-1325 (1995)

Haruna,H：“SAMP1 小鼠 B 细胞和树突状细胞的异常。”