Proteomic analysis of the invading mechanism of Neospora caninum and development of novel preventive and treatment methods

犬新孢子虫入侵机制的蛋白质组学分析及新型预防和治疗方法的开发

• 批准号: 18380180
• 负责人: XUAN Xuenan
• 金额: $ 11.87万
• 依托单位: Obihiro University of Agriculture and Veterinary Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18380180/
• 关键词: ネオスポラ; トキソプラズマ; プロテオミックス; ワクチン; 治療薬; MALDI-TOF-MS; 抗原; 酵素; Neospora caninum; Toxoplasma gondii; 感染機構; プロテオーム; 薬剤標的分子; 組換えワクチン; 抗原虫薬; 治療; 予防; 抗原タンパク質

The aims of this study were to widen our view on the global expression of protein profile of Neospora caninum parasite and to discover vaccine and drug target molecules for control of neosporosis. Approximately 600 protein spots were detected on 2-DE, and 60 proteins were identified by MALDI-TOF-MS analysis. Some of the identified proteins were predicted to be involved in the invasion of the parasite into the host cells and in parasite metabolism. Therefore, some of these proteins could be potential vaccine candidates and drug targets.

这项研究的目的是扩大我们对新孢子虫寄生虫蛋白质谱的全球表达的看法，并发现疫苗和药物靶标分子以控制新孢子虫病。在2-DE上检测到大约600个蛋白质斑点，并通过MALDI-TOF-MS分析鉴定了60个蛋白质。预计某些已鉴定的蛋白质与寄生虫侵入宿主细胞和寄生虫代谢有关。因此，其中一些蛋白质可能是潜在的候选疫苗和药物靶标。

项目成果

Toxoplasma gondii Cyclophilin 18-Mediated Production of Nitric Oxide Induces Bradyzoite Conversion in a CCR5-Dependent Manner

• DOI: 10.1128/iai.00361-09
• 发表时间: 2009-09-01
• 期刊: INFECTION AND IMMUNITY
• 影响因子: 3.1
• 作者: Ibrahim, Hany M.;Bannai, Hiroshi;Nishikawa, Yoshifumi
• 通讯作者: Nishikawa, Yoshifumi

ネオスポラ特異的移行抗体は原虫感染の防御に関与しない

新孢子虫特异性过渡抗体不参与针对原生动物感染的保护

• 发表时间: 2010
• 作者: 西川義文;ら
• 通讯作者: ら

Toxoplasma gondii の全身伝搬における血中 free tachyzoite と抹消血細胞感染感染 tachyzoite の関与

弓形虫全身传播中血液中游离速殖子和外周血细胞感染速殖子的参与

• 发表时间: 2007
• 作者: 海野明宏;玄学南;西川義文;鈴木和彦;鬼頭克也;高島康弘
• 通讯作者: 高島康弘

Characterizaion of a leucine aminopeptidase from Toxoplasma gondii

弓形虫亮氨酸氨肽酶的表征

• 发表时间: 2010
• 期刊: Mol. Biochem. Parasitol. 170
• 作者: Jia;H.;Nishikawa;Y.;Luo;Y.;Yamagishi;J.;Sugimoto;C.;Xuan;X.
• 通讯作者: X.

Identification of a protein disulfide isomerase of Neospora caninum in excretory-secretory products and its IgA binding and enzymatic activities.

• DOI: 10.1016/j.vetpar.2006.02.029
• 发表时间: 2006-06
• 期刊: Veterinary parasitology
• 影响因子: 2.6
• 作者: M. Liao;Liqing Ma;H. Bannai;Eung‐goo Lee;Z. Xie;Xiaofei Tang;Houshuang Zhang;X. Xuan;K. Fujisaki