Control of Criptosporidium parvum infection in calves by recombinant herpesvirus vector vaccine

重组疱疹病毒载体疫苗控制犊牛隐孢子虫感染

• 批准号: 10660297
• 负责人: XUAN Xuenan
• 金额: $ 2.05万
• 依托单位: Obihiro University of Agriculture and Veterinary Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10660297/
• 关键词: C.parvum; P15; P23; Recombinant vaccine; Vaccinia virus; Herpesvirus; 原虫感染防御; 組換え抗原; クリプトスポリジウム; ウイルスベクター; P23遺伝子; P15遺伝子; ワクチン; C.parvum; P15; P23; Recombinant vaccine; Vaccinia virus; Herpesvirus; 原虫感染防御; 組換え抗原; クリプトスポリジウム; ウイルスベクター; P23遺伝子; P15遺伝子; ワクチン

In host-parasite interaction, the surface proteins of both host and parasite cells play an important role and the surface antigens of the parasites are therefore logical targets for subunit vaccine development. Several surface antigens, such as P15 and P23 of Cryptosporidium parvum (C.parvum) sporozoites or merozoites have been reported previously. Since the monoclonal antibodies against these antigens can neutralize the C.parvum sporozoites, the antigens were proposed as candidates for development of recombinant vaccine to control the cryptosporidiosis. In the present study, we constructed recombinant vaccinia virus or herpesvirus wich expresses the P15 or P23 of C.parvum and demonstrated that the recombinant P15 and P23 are similar to the native P15 and P23 both in structure and antigenicity. Mice or rabbits inoculated with the recombinant viruses produced high titers of antibodies against C.parvum sporozoites. Our next step will be immunization trails with cows to check the potency of the hyperimmune colostrum to confer effective passive immunity of humans and animals against cryptosporidiosis.

在宿主 - 寄生虫相互作用中，宿主和寄生虫细胞的表面蛋白都起着重要作用，因此，寄生虫的表面抗原是亚基疫苗发育的逻辑靶标。先前已经报道了几种表面抗原，例如p15和p23的孢子虫（C. parvum）的孢子虫或梅洛寄生。由于针对这些抗原的单克隆抗体可以中和孢子孢子念珠菌，因此提出抗原作为开发重组疫苗以控制隐孢子虫病的候选者。在本研究中，我们构建了重组离发病毒或疱疹病毒的表达c.parvum的p15或p23，并证明了重组p15和p23与在结构和抗原性中的天然p15和p23相似。被重组病毒接种的小鼠或兔子产生了针对孢子菌的抗体的高滴度。我们的下一步将是用母牛进行免疫道路，以检查高免疫初乳的效力，以允许人类和动物的有效的被动免疫免受隐孢子虫病的影响。