Epidemiological investigation of protozoan diseases in China

我国原虫病流行病学调查

• 批准号: 13575030
• 负责人: XUAN Xuenan
• 金额: $ 8.32万
• 依托单位: Obihiro University of Agriculture and Veterinary Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13575030/
• 关键词: Epidemiology; recombinant antigens; T.gondii; N.canunim; C.parvum; B.caballi; B.equi; B.gibsoni; クリストスポリジウム感染症; ELISA; 中国新疆地区; 中国吉林地区

1)Cloning of genes encoding immunodominant antigens of protozoan parasitesIn the present study, many genes encoding irnmunodominat antigens of protozoan parasites were identified, cloned and characterized. The main genes are as follows : B.caballi P48, P134;B.equi EMA-1,EMA-2,P82;B.gibsoni P18,P29,P50;N.caninum SAG1,SRS2;T.gondii SAG1,SAG2,SRS1,ROP-1.2)Development of serodiagnostic methods for detection of antibodies to protozoan parasitesThe genes encoding immunodominant antigens were expressed in E.coli or insect cells, and then established the enzyme-linked immunosorbent assay, latex agglutination test, and immunochromatographic test with these recombinant antigens.3)Development of polymerase chain reactions for detection of protozoan parasitesThe primer sets based on genes described above were designed, and established PCR methods for detection of protozoan parasites.4)Epidemiological investigation of protozoan diseases in ChinaBlood samples collected from horses, cattle, pigs, sheep, and dogs, m Shinjiang, Jilin, Beijing, Fujian, and Tibet provinces, China, respectively, were examined for the diagnoses of babesiasis, toxoplasmosis, neosporosis, and cryptosrondiosis, and obtained many new findings.

1）编码本研究的原生动物寄生虫免疫抗原的基因克隆，鉴定了，克隆和表征许多编码原生动物寄生虫的IRNMUNODOMINAT抗原的基因。主要基因如下：b.caballi p48，p134; equi ema-1，ema-2，p82; b.gibsoni p18，p29，p29，p50; n.carinum sag1，srs2; t.gondii sag1，sag1，sag2，srs1，srs1，srs1，rop-1.2）在大肠杆菌或昆虫细胞中表达了编码免疫抗原的寄生基因，然后建立了酶 - 连接的免疫吸收测定法，乳胶凝集试验，乳胶凝集试验，并通过这些重组抗原的开发基于原子质酶的基于原子能化杂种的开发，并使用这些重组抗原进行了免疫色谱测试。已建立的PCR方法用于检测原生动物的寄生虫。4）流行病学研究对从马，牛，牛，猪，绵羊和狗收集的中心样品中的原生动物疾病，M Shinjiang，Mhinjiang，Jilin，Jilin，北京，北京，福吉安，福吉安和藏族省份，中国诊断为诊断，造成了诊断的症状，并诊断为隐孢子虫病，并获得了许多新发现。

项目成果

Hirata, H., et al.: "Identification of a specific antigenic region of P82 of Babesia equi and its potential use in serodiagnosis"J.Clin.Microbiol.. 41・1. 547-551 (2003)

Hirata, H., et al.：“马巴贝虫 P82 的特定抗原区域的鉴定及其在血清诊断中的潜在用途”J.Clin.Microbiol.. 41・1 (2003)。

Huang, X., et al.: "High-level expression and purification of a truncated EMA-2 of Babesia equi in Escherichia coli and its potential for immunodiagnosis."J.Clin.Microbiol.. 41・3. 1147-1151 (2003)

Huang, X., et al.：“马巴贝虫的截短 EMA-2 在大肠杆菌中的高水平表达和纯化及其免疫诊断潜力。”J.Clin.Microbiol.. 41・3。 2003）

Huang, X., Xuan, X., Yokoyama, N., Xu, L., Suzuki, H., Sugimoto, C., Nagasawa, H., Fujisaki, K., Igarashi, I.: "High-level expression and purification of a truncated merozote antigen-2 of Babesia equi in Escherichia coli and its potential for immunodiagno

Huang, X., Xu, X., Yokoyama, N., Xu, L., Suzuki, H., Sugimoto, C., Nagasawa, H., Fujisaki, K., Igarashi, I.：“高级表达

Xuan, X., et al.: "Detection of antibodies to Babesia equi in horses by a latex agglutination test using recombinant EMA-1."Clin.Diagn.Lab.Immunol.. 8・3. 645-646 (2002)

Xu, X., et al.：“通过使用重组 EMA-1 的乳胶凝集试验检测马体内马巴贝虫抗体”。Clin.Diagn.Lab.Immunol.. 8・3（2002）。

Hirata, H., et al.: "Cloning of a truncated Babesia equi gene encoding an 82-kDa protein and its potential use in an ELISA"J.Clin.Microbiol.. 40・4. 1470-1474 (2002)

Hirata, H., et al.：“编码 82-kDa 蛋白质的截短马巴贝虫基因的克隆及其在 ELISA 中的潜在用途”J.Clin.Microbiol.. 40·4 (2002)。