Study of the mucosal immunomoduLation by intestinal commensal bacteria

肠道共生菌对粘膜免疫调节的研究

• 批准号: 18380084
• 负责人: KAMINOGAWA Shuichi
• 金额: $ 10.42万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18380084/
• 关键词: allergy; intestinal microbacteria; immunology; gene; 腸内共生細菌; IgA; T細胞応答; 免疫関連遺伝子

Intestinal commensal bacteria play important roles in the regulation of intestinal immune responses such as anti-allergic reaction and anti-infection. It has been shown that intestinal commensal bacteria greatly affect the development of gut-associated lymphoid tissues and mucosal immune responses, such as IgA production, in the gut. Given the large numbers of intestinal microbiota, particularly in the large intestine, it is believed that immunocytes in the large intestine are modulated by microbacteria. While IgA production by immunocytes in the small intestine has been previously studied, IgA production in the large intestine is, however, poorly understood. In this study, we show that IgA production by lymphocytes is induced by microbacteria present in the large intestine.Lamina propria lymphocytes from the large intestine (L-LP) were isolated from germ-free (GF) and conventional (CV) mice. Flow cytometric analysis of L-LP lymphocytes was used to assess the frequency of IgM+B220+ cel … More ls, IgA+B220+ cells, and IgA+Syndecan-l+B220- cells. In addition, to determine whether stimulation by commensal bacteria influences differentiation of IgM+ cells into IgA-plasma cells, IgM+ cells separated from L-LP lymphocytes of GF mice were co-cultured with Bacteroides acidofaciens isolated from intestinal commensal bacteria of CV mice.The frequency of IgA+Syndecan-1+B220- cells and IgA+B220+ cells in L-LP of GF were all lower than that of CV mice, but there was no difference in the frequency of IgM+13220+ cells in L-LP between GF and CV mice. IgA production by IgM+ cells stimulated by B. acidofaciens was higher than in those without bacterial stimulation.These results indicate that direct stimulation by B. acidofaciens induces differentiation of IgM+ cells into IgA-producing plasma cells from the large intestine. These also suggest that IgA production in the large intestine might be induced by intestinal commensal bacteria, thus intestinal microbacteria promote the class switching from IgM to IgA in the large intestine. Less

肠道细菌在调节肠道免疫反应（例如抗过敏反应和抗感染）中起着重要作用。已经表明，肠道细菌极大地影响了肠道中与肠道相关的淋巴组织和粘膜免疫反应（例如IgA产生）的发展。考虑到大量肠道菌群，尤其是在大肠中，据信大肠中的免疫细胞受微细菌调节。虽然先前已经研究了小肠中免疫细胞的IgA产生，但大肠中的IgA产生知之甚少。在这项研究中，我们表明淋巴细胞的IgA产生是由大肠中存在的微单细菌诱导的。从无菌（GF）和常规（CV）小鼠中分离出来自大肠（L-LP）的层次淋巴细胞（L-LP）。 L-LP淋巴细胞的流式细胞仪分析用于评估IgM+B220+Cel的频率…更多LS，IgA+B220+细胞和IgA+Syndecan-L+B220-细胞。另外，为了确定相当细菌的刺激是否影响IgM+细胞分化为IgA-铂 - - 铂型细胞，将与GF小鼠的L-LP淋巴细胞分离的IgM+细胞与与CV MITE的肠道+b220+iga+syndeca+syndedededeca+syndededecaNdeDecain+syndededecain+syndedededecaimefaciens共培养的IgM+细胞。 GF的L-LP中的细胞均低于CV小鼠的细胞，但是GF和CV小鼠之间L-LP中IgM+ 13220+细胞的频率没有差异。由酸性芽孢杆菌刺激的IgM+细胞产生的IgA高于没有细菌刺激的IgA。这些结果表明，酸性芽孢杆菌的直接刺激会影响IgM+细胞的分化为来自大肠的产生IgA产生的浆细胞。这些还表明，大肠中的IgA产生可能是由肠道菌群引起的，因此肠道微细菌促进了大肠中从IgM转换为IgA的类别。较少的

项目成果

Modulation of cytokine and immunoglobulin A release bybeta-(1,3-1,6)-glucan from Aureobasidium ullulans strain IA 1

Aureobasidium ullulans 菌株 IA 1 中 β-(1,3-1,6)-葡聚糖对细胞因子和免疫球蛋白 A 释放的调节

• 发表时间: 2006
• 期刊: Animal Cell Technology:Basic&Applied Aspects 14
• 作者: Suzuki T;Hosono A;Hachimura S;Suzuki T;Kaminogawa S
• 通讯作者: Kaminogawa S

Increase in terminal fragments of 16S rRNA genes derived from Bacteroidetes after administratin of short-chain fructooligosaccharides.

给予短链低聚果糖后，来自拟杆菌门的 16S rRNA 基因末端片段增加。

• 发表时间: 2006
• 期刊: Appl. Environ. Microbiol. 72
• 作者: Nakanishi Y;Murashima K;Ohara H;Suzuki T;Hayashi H;Sakamoto M;Fukusawa T;Kubota H;Hosono A;Kono T;Kaminogawa S;Benno Y
• 通讯作者: Benno Y

The role of CD4^+T cells in IgA production in murine Peyer's patches followin oral feeding of Bifidobacterium components

口服双歧杆菌成分后 CD4^ T 细胞在小鼠派尔氏集结中 IgA 产生中的作用

• 发表时间: 2006
• 期刊: Animal Cell Technology:Basic&Applied Aspects 14
• 作者: Nakanishi Y;Hosono A;Kimura T;Kaminogawa S
• 通讯作者: Kaminogawa S

The role of CD4^+ T cells in IgA production in murine Peyer's patches following oral feeding of Bifidobacterium components.

口服双歧杆菌成分后，CD4^T 细胞在小鼠派尔氏集结中 IgA 产生中的作用。

• 发表时间: 2006
• 期刊: Animal Cell Technology : Basic ＆ Applied Aspects, 14
• 作者: Nakanishi Y;Hosono A;Kimura T;Kaminogawa S
• 通讯作者: Kaminogawa S

調製法の異なるBifidobacterium菌体成分が修飾する免疫応答の特

不同制备方法双歧杆菌细胞成分修饰免疫反应的特点

• 发表时间: 2007
• 作者: 平松 靖浩;細野 朗;高橋 恭子;上野川 修一
• 通讯作者: 上野川 修一