Purification, identification and functional analysis of immune suppressive factol

免疫抑制因子的纯化、鉴定及功能分析

• 批准号: 02454065
• 负责人: KAMINOGAWA Shuichi
• 金额: $ 4.03万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-02454065/
• 关键词: immune suppressive factor; IL-10; suppressor T cell; immune tolerance; food allergen; 抑制因子

CD8+ suppressor T cell clone 13G2 was established from lymph node of bovine si-casein immunized C57BL/6 mice. The clone suppressed proliferation of Thl clones induced by antigen plus antigen-presenting cells (APC) without any detectable cytolytic activity for responding cells. The suppression was not antigen specific and was not restricted to MHC. However, the clonec did not inhibit IL-2 induced Thl-proliferatiol) (1). The supernatant of 13G2 cultured with rIL-2 also suppressed antigen-induced proliferation of Thl clones, but did not that of Th2 clones. Target cells for the suppressor function was APC. Anti-IL-lO antibody SXC-I. blocked this suppressive activity. 13G2 clone produced IL-10 as revealed by Western blot analysis. This indicates that the suppressive lymphokine produced by 13G2 clone is IL-10 (2). A PCR analysis has shown that CD8+ cells of immunized lymph node express IL-10 mRNA upon antigenic stimulation. It is. possible that IL-10 play an important role in the immune suppression mediated by CD8+ lymphocytes.(1) T. Hisatsune et al. (1990) J. lmmunol., 145 : 2421(2) T. Hisatsune et al. (1992) Lymphokine & Cytokine Res., in press

CD8+抑制剂T细胞克隆13G2是从牛Si-Casein免疫C57BL/6小鼠的淋巴结中建立的。该克隆抑制了抗原加抗原呈递细胞（APC）诱导的THL克隆的增殖，而没有任何可检测到的溶细胞活性来响应细胞。抑制不是抗原特异性的，也不限于MHC。但是，CLONEC并未抑制IL-2诱导的Thl-prostrohatiol）（1）。用RIL-2培养的13G2的上清液也抑制了抗原引起的THL克隆的增殖，但没有Th2克隆。抑制器功能的靶细胞为APC。抗IL-LO抗体SXC-I。阻止了这种抑制作用。 13G2克隆产生了通过Western印迹分析揭示的IL-10。这表明由13G2克隆产生的抑制性淋巴因子为IL-10（2）。 PCR分析表明，抗原刺激后免疫淋巴结的CD8+细胞表达IL-10 mRNA。这是。 IL-10在CD8+淋巴细胞介导的免疫抑制中起重要作用。（1）T。Hisatsune等。 （1990）J。Lmmunol。，145：2421（2）T。Hisatsune等。 （1992）淋巴因子和细胞因子res。

项目成果

Hisatsune T.: "A suppressive lymphokine derived from Ts clone 13G2 is ILー10" Lymphokine and Cytokine Research. 11. 87-93 (1992)

Hisatsune T.：“源自 Ts 克隆 13G2 的抑制性淋巴因子是 IL-10”，淋巴因子和细胞因子研究，11. 87-93 (1992)。

Hisatsune T.: "A suppressive lymphokine derived from Ts clone 13G2 is IL-10" Lymphokine and Cytokine Research. 11. 87-93 (1992)

Hisatsune T.：“源自 Ts 克隆 13G2 的抑制性淋巴因子是 IL-10”淋巴因子和细胞因子研究。