The analysis of the epitope structures of food allergens by using the synthetic peptides.

利用合成肽分析食物过敏原的表位结构。

• 批准号: 62560115
• 负责人: KAMINOGAWA Shuichi
• 金额: $ 1.34万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62560115/
• 关键词: Antibody; T cell; Casein; エピトープ; モノクローナル抗体; β-ガゼイン; 抗原抗体反応; ELISA; NMR; αs_1-カゼイン; 牛乳アレルギー

It is important for prevention and cure of food allergy to elucidate the epitope structures of the allergens. In this study, we examined the structures of casein, one of potent allergens in bovine milk.The full profiles of the sites on bovin s1-casein ( s1-CN) recognized by T and B cells were determined by using synthetic overlapping peptides and enzymatically digested fragments. It was shown that 1) limited sites on s1-CN are recognized by T and B cells; 2) the profiles of these recognition sites on s1-CN are similar in three mouse strains that have independent haplotypes.The interaction between monoclonal antibody (MAb1C3) to -casein ( -cn) and its fragments was analyzed at the molecular level. As a result, it was shown that the amino acid residues, 194Gln, 200Pro and 202Arg, contributed largely to the binding to MAb1C3 and the contributions of 193Tyr and 201Val were inferior to the formers.This information on the epitope structure of food allergens may be useful in understandind the antigen recognition in the immune response as well as the hypersensitivity in food allergy .

对于预防和治愈食物过敏至关重要，以阐明过敏原的表位结构。在这项研究中，我们检查了酪蛋白的结构，其中一种是牛奶中有效的过敏原。通过使用合成重叠的肽和酶促消化的片段来确定由T和B细胞识别的牛S1-酶（S1-CN）的完整特征。结果表明，1）S1-CN上的有限位点被T和B细胞识别； 2）在具有独立单倍型的三种小鼠菌株中，S1 -CN上这些识别位点的曲线相似。单克隆抗体（MAB1C3）与Casein（-cn）（-cn）及其片段之间的相互作用在分子水平上进行了分析。结果，结果表明，氨基酸残基194GLN，200PRO和202ARG在很大程度上有助于与MAB1C3的结合以及193tyr和2011VAL的贡献较低。对食品过敏原结构的信息可能有助于理解食品过敏蛋白的结构，可以理解抗原识别性的抗原疗法以及免疫疗法以及催眠剂以及与催眠者以及抗毒性相同。

项目成果

A. Enomoto; Y. Aoki; J. Kurisaki; S. Kaminogawa; K. Yamauchi: "T cell response to hen's egg ovomucoid." Agric. Biol. Chem.52. 2531-2536 (1988)

A.榎本；

T.Takahashi,;S.Kaminogawa,;T.Kuwata,;K.Yamauchi.: Agric.Biol.Chem.52. 2485-2491 (1988)

T.Takahashi,;S.Kaminokawa,;T.Kuwata,;K.Yamauchi.: Agric.Biol.Chem.52。

T. Takahashi; S. Kaminogawa; T. Kuwata; K. Yamauchi: "T-cell recognition of -lactoglobulin." Agric. Biol. Chem.52. 2485-2491 (1988)

T.高桥；

S.Nagaune,;S.Kaminogawa,;A.Enomoto,;T.Kobayashi,;J.Kurisaki,;K.Yamauch.: Agric.Bilo.Chem.52. 2577-2581 (1988)

S.Nagaune,;S.Kaminokawa,;A.Enomoto,;T.Kobayashi,;J.Kurisaki,;K.Yamauch.：Agric.Bilo.Chem.52。

S. Nagaune; S. Kaminogawa; A. Enomoto; T. Kobayashi; J. Kurisaki; K. Yamauchi: "Preparation of anti-bovine -casein monoclonal antibody and analysis of the interaction between the antibody and -casein fragment." Agric. Biol. Chem.52. 2577-2581 (1988)

S.Nagaune;