Studies on antioxidant activities of bilirubin and in vivo lipid peroxidation of fish.

鱼类胆红素抗氧化活性及体内脂质过氧化作用的研究。

基本信息

批准号：
07660272
负责人：
SAKAI Tadashi
金额：
$ 1.41万
依托单位：
Miyazaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07660272/
关键词：
Bilirubin Jaundice Yellowtail Substance X alpha-Tocopherol Ascorbic Acid Extruder Treatment Fish Meal

项目摘要

1.Yellowtail Seriola quinqueradiata were fed on extruder diets (EP) for 2 month and unjected bacteria (JB) whose infection is thought to one of the cause of jaudice. The injection induced to hemolysis. Hemolysis occurred bring about in vivo lipid peroxidation and alpha-tocopherol (alpha-Toc) and ascorbic acid (ASA) were consumed to prevent in vivo lipid peroxidation.2.Yellowtail were fed on EP containing different POV,alpha-Toc and ASA levels of sardine oil for 2 months and the phenyl hydorazine and JB.2-Thiobarbituric acid reactive substances (TBARS) values and phospholipids hydroperoxides (PH) contents of fish fed on diet containing high levels of alpha-Toc and ASA were much lower than those of fish fed on EP containing low level of alpha-Toc and ASA.Plasma bilirubin level of fish fed on diet containing high level of alpha-Toc and ASA were much lower than those of fish fed on diet containing low of alpha-Toc and ASA.3.Simultaneous determination of ascorbic acid (AA) and dehydroascorb … More ic acid (DHA) in fish tissues has been developed by high performance liquid chromatography with postcolumn derivatization method. AA and DHA extracted were separated by a Shim-pack SCR-101H column within 20 min, reacted with sodium hydroxide containing sodium borohydride and monitored at 300nm. The detection limits for both AA and DHA were 0.1 mug/ml.4.Yellowtail were fed on EP containing high level of L-Ascorby1-2-phosphate Mg (APM) and ASA for 2 months (group H) or low level of APM and ASA (group L). The plasma and liver TBARS value and PH contents were lower in group H than in group L.Liver ASA contents in group H was higher than those in group L but plasma ASA levels were similar to both qroups When JB was injected to the fish plasma bilirubin levels, plasma bilirubin levels were similar to both groups. These results suggest that plasma ASA level was not enough to prevent jaundice of group H.5.Coho salmon was injected with erythrocytic inclusion body syndrome (EIBS) virus. Plasma bilirubin levels were slightly increased. Judging from the liver TBARS values and fatty acid composition of erythrocytes membrane, in vivo lipid peroxidation was occurred in the fish. Less

1.鰤鱼饲喂挤压饲料（EP）2个月，未注射细菌（JB），其感染被认为是引起黄疸的原因之一，注射引起溶血，导致体内脂质过氧化和α。 -消耗生育酚（α-Toc）和抗坏血酸（ASA）以防止体内脂质过氧化。2.黄尾鱼饲喂含有不同 POV、α-Toc 和 ASA 水平的沙丁鱼油 EP 2 个月，以及饲喂鱼的苯肼和 JB.2-硫代巴比妥酸反应物质 (TBARS) 值以及磷脂氢过氧化物 (PH) 含量含有高含量 alpha-Toc 和 ASA 的鱼远低于喂食含有低含量 alpha-Toc 和 ASA 的 EP 的鱼。血浆胆红素水平饲喂高α-Toc和ASA的饲料的鱼明显低于饲喂低α-Toc和ASA的饲料的鱼。 3.抗坏血酸（AA）和脱氢抗坏血酸（DHA）的同时测定）通过高效液相色谱法和柱后衍生法开发了鱼组织中提取的 AA 和 DHA，并通过 Shim-pack SCR-101H 柱进行分离。 20 分钟，与含有硼氢化钠的氢氧化钠反应，并在 300nm 处监测。AA 和 DHA 的检测限均为 0.1 微克/毫升。4. 黄尾鱼饲喂含有高浓度 L-Ascorby1-2-磷酸镁 (APM) 的 EP。 ) 和 ASA 2 个月 (H 组) 或低水平 APM 和 ASA (L 组) 组血浆和肝脏 TBARS 值和 PH 含量较低。 H组肝脏ASA含量高于L组，但血浆ASA水平与两组相似。当给鱼注射JB时血浆胆红素水平，血浆胆红素水平与两组相似。提示血浆ASA水平不足以预防H.5组的黄疸。银鲑鱼注射红细胞包涵体综合征(EIBS)病毒后血浆胆红素水平略有升高。从肝脏TBARS值和红细胞膜脂肪酸组成来看，鱼体内发生脂质过氧化的情况较少。