Studies on stimulation of feeding behavior in yellowtail Seriola quinqueradiata.

鰤鱼摄食行为刺激的研究。

基本信息

批准号：
07660243
负责人：
HIDAKA Iwao
金额：
$ 1.41万
依托单位：
Mie University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07660243/
关键词：
Fish Yellowtail Feeding stimulants Muscle extracts Taste Olfaction 魚類エキス

项目摘要

The feeding-stimulatory effects on the yellowtail Seriola quinqueradiata of extracts of the jack mackerel Trachurus japonicus white muscle were studied. Ultrafiltrates of water extracts of the muscle (M.W.cut off 10,000) were charged on a DEAE-Sephadex A-25 at pH 5.5 or CM-Sephadex C-25 at pH 6.5. Effluents and eluates with 0.6M NaCl were freeze-dried and the components were applied in starch pellets to yellowtails, 23-31g in body weight. Their feeding-stimulatory effectiveness was evaluated by counting the number of pellets eaten within the first minute after application.Their stimulatory effects on the gustatory and olfactory receptors of the yellowtail, 230-746g in b.w., were also studied by recording the electrical responses from the palatine taste nerve and olfactory bulb.(1) Feeding-stimulatory effectiveness of fractioned components (a) Components of the effluent through DEAE-Sephadex column were ineffective, while those of the eluate with NaCl were slightly less effective than t … More he untreated extract. (b) Stepwise elution with 0.2M and 0.6M NaCl on the DEAE-Sephadex column yielded two less effective clusters of different components : 0.2M NaCl-eluate contained lactic acid and minhydrin positive compounds, and 0.6M NaCl-eluate inosinic acid. (c) Components of the eluate from CM-Sephadex C-25 were ineffective, while those of the effluent were nearly as effective as the total extract.(2) Gustatory and olfactory neural responses to fractioned components (a) Both gustatory and olfactory systems responded more or less to all fractions tested. (b) The threshold for the total extract was lower in olfactory system than in gustatory system by some three orders. That for some fractions was also so to different degrees while that for others was similar to each other between the two systems. (c) The threshold for both of lactic acid and inosinic acid, present in 0.2M and 0.6M NaCl-eluates from DEAE-Sephadex culumn respectively and considered one of important components, was lower in gustatory system. Less

研究了竹荚鱼 Trachurus japonicus 白肌肉提取物对黄尾鰤的摄食刺激作用。将肌肉水提取物的超滤液（M.W.截止 10,000）加载到 pH 5.5 或 5.5 的 DEAE-Sephadex A-25 上。 CM-Sephadex C-25，pH 6.5，流出液和洗脱液为 0.6M。将氯化钠冷冻干燥，并将这些成分以淀粉颗粒的形式施用到体重为23-31克的鰤鱼身上，通过计算施用后第一分钟内吃下的颗粒的数量来评估它们的摄食刺激效果。它们对味觉的刺激作用。还通过记录腭味神经和嗅觉的电反应来研究黄尾鱼的嗅觉受体，体重为 230-746g。 (1) 分馏成分的摄食刺激效果 (a) 通过 DEAE-Sephadex 柱的流出液成分无效，而含有 NaCl 的洗脱液成分的效果略低于未经处理的提取物 (b) 逐步法。在 DEAE-Sephadex 柱上用 0.2M 和 0.6M NaCl 洗脱产生两个不同组分的效率较低的簇： 0.2M NaCl 洗脱液含有乳酸(c) CM-Sephadex C-25 的洗脱液成分无效，而流出液的成分几乎与总提取物一样有效。(2) 味觉和味觉。对分级成分的嗅觉神经反应 (a) 味觉和嗅觉系统对所有测试的级分或多或少都有反应 (b) 总提取物的阈值是。嗅觉系统中的阈值比味觉系统中的阈值低约三个数量级。对于某些部分，这两个系统的阈值也有不同程度的变化，而另一些部分的阈值则彼此相似。分别存在于 DEAE-Sephadex 柱的 0.2M 和 0.6M NaCl 洗脱液中，被认为是重要成分之一，在味觉系统中含量较低。