Studies on stimulation of feeding behavior in yellowtail Seriola quinqueradiata

鰤鱼摄食行为刺激研究

基本信息

批准号：
03660190
负责人：
HIDAKA Iwao
金额：
$ 1.34万
依托单位：
Mie University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03660190/
关键词：
Fish Yellowtail Feeding stimulants Extracts Taste

项目摘要

The feeding-stimulatory effectiveness of a water extract of the jack mackerel Trachurus japonicus white muscle for the young yellowtail Seriola quinqueradiata was studied with the aid of gel chromatography, ion exchange chromatography and other analytical methods. The feeding-stimulatory effectiveness of test fractions of the extract was evaluated by applying them to the young yellowtail in starch pellets and counting the number of pellets eaten by the fish. (1) Gel fractions of the extracts on a column of Sephadex-15 The water extract of the muscle was ultrafiltrated through a filter membrane (UK-15, Advantic, M.W. cut off 10,000) and the ultrafiltrate was fractionated on a column of Sephadex G-15 from 0.05 M sodium chloride. The fractions obtained were divided into groups and pooled. Then the pooled eluate was freeze-dried and the residues were dissolved in sea water and applied to the fish. The results showed that effective compounds were included in fractions of molecular weight of several hundreds or lower. (2) Fractions of the extracts obtained by treating with DEAE-Sephadex and CM-Sephadex The ultrafiltrate of the muscle extract was treated with (a) DEAE-Sephadex A-25 anion exchanger, (b) CM-Sephadex C-25 cation exchanger or (c) both of the two ion exchangers in batch procedures. The results obtained showed that (a) reduced the stimulatory effectiveness of the extract. This suggests that some of the effective constituents are adsorbed to the DEAE-Sephadex;(b) had no apparent influence on the effectiveness of the extract; (c) resulted in a sever depression of the effectiveness, suggesting that constituents adsorbing to the cation exchanger also show a feeding- stimulatory effect in cooperation with constituents in the (c)-treated extract.

借助凝胶色谱、离子交换色谱等分析方法，研究了竹荚鱼白肌水提取物对鰤幼鱼的摄食刺激效果。通过将提取物的测试部分应用于淀粉颗粒中的幼鰤鱼并计算鱼吃掉的颗粒数量来评估提取物测试部分的摄食刺激效果。 (1) 提取物在 Sephadex-15 柱上的凝胶级分将肌肉的水提取物通过滤膜（UK-15，Advantic，M.W. cut off 10,000）进行超滤，并将超滤液在 Sephadex G 柱上分级-15，来自 0.05 M 氯化钠。将获得的级分分组并合并。然后将合并的洗出液冷冻干燥，将残留物溶解在海水中并施用于鱼。结果表明，有效化合物包含在分子量数百或更低的部分中。 (2)通过用DEAE-Sephadex和CM-Sephadex处理获得的提取物的级分用(a)DEAE-Sephadex A-25阴离子交换剂、(b)CM-Sephadex C-25阳离子交换剂处理肌肉提取物的超滤液或 (c) 两种离子交换剂均采用间歇程序。获得的结果表明 (a) 降低了提取物的刺激效果。这表明部分有效成分被DEAE-Sephadex吸附；(b)对提取物的有效性没有明显影响； (c)导致有效性的严重降低，这表明吸附到阳离子交换剂上的成分也显示出与(c)处理的提取物中的成分配合的摄食刺激作用。