Histological damage oflung allografts according to the magnitude of acute rejection in the reiso-transplant model

根据 reiso 移植模型中急性排斥程度的同种异体肺移植物的组织学损伤

• 批准号: 18591543
• 负责人: IWATA Hisashi
• 金额: $ 2.4万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591543/
• 关键词: Pulmonary surgery; lune transnlantation; lung regeneration; 拒絶反応

ABSTRACTBackground: Graft damage due to acute rejection has been reported to be one of the risk factors in the chronic stage of cardiac and renal allografts. This study was designed to elucidate the histological changes of grafts after ongoing acute allograft rejection was discontinued in lung re-iso-transplantation models.Methods: WKAH rat lungs were orthotopically transplanted into F344 recipients. Three days (Group 3A) and 5 days (Group 5A) after the first allotransplantation, the grafts were re-iso-transplanted back into the WKAH rats (Group 3RA and Group 5RA, respectively). Five days (Group 5I) after the first isotransplantation, the grafts were re-iso-transplanted back into the WKAH rats (Group 5RI). The grafts were removed 30 and 60 days after re-iso-transplantation and assessed histologically.Results: Typical acute allograft rejection developed in the 3A and 5A groups, and the changes were reduced after re-iso-transplantation, though they remained significantly greater in the 5RA group than in the 3RA and SRI groups. In intimal hyperplasia, the score of the graft 60 days after re-iso-transplantation in the 5RA group was significantly higher than in the SRI and 3RA groups. The changes in airway inflammation were significantly greater in the 5RA group than in the 3RA and SRI groups at 60 days. Peribronchiolar fibrosis was significantly greater in the 5RA and 3RA groups than in the SRI group.Conclusions: Acute rejection and airway inflammation corresponded to the magnitude of rejection before retransplantation; significant intimal hyperplasia developed in severe acute rejection, and peribronchiolar fibrosis occurred after the first acute rejection.

抽象背景：据报道，由于急性排斥而引起的移植物损害是心脏和肾脏同种异体移植的慢性阶段中的危险因素之一。这项研究旨在阐明持续的急性同种异体移植排斥反应在肺部再ISO转移模型中停用后移植物的组织学变化。方法：wkah大鼠肺是原始人移植到F344受体中的。第一次同种异体移植后为期三天（第3A组）和5天（第5A组），将移植物重新移植回WKAH大鼠（分别为第3RRA组和第5RA组）。五天（第5i组）在第一次各向同移植后，将移植物重新置于WKAH大鼠（第5RI组）中。移植后30天和60天去除移植物并在组织学上进行评估。回归：在3A和5A组中产生的典型急性急性同种异体移植抑制，并且在重新转移后的变化降低，尽管在3RA组中，它们在5RA组中的保留程度明显更大。在内膜增生中，5RA组重新转移后60天的移植物分数显着高于SRI和3RA组。 5RA组的气道炎症变化明显高于60天的3RA和SRI组。在5RA和3RA组中，周元纤维化明显大于SRI组。结论：急性排斥反应和气道炎症对应于转染前的排斥幅度。严重的急性排斥反应出现了明显的内膜增生，并且第一次急性排斥反应后发生了纤维隆纤维化。

项目成果

Histological damage of lung allografts according to the magnitude of acute rejection in the re-iso-transplant model

根据再异移植模型中急性排斥程度的同种异体肺移植物的组织学损伤

• 期刊: Journal of Heart and Lung Transplantation In Press
• 作者: Kyoichi Takaori;Tomoyuki Agui;Yoshiharu Miyamoto;Mitsuhiko Iwamoto;Hisashi Watanabe;Nobuhiko Tanigawa;Yutaro Egashira;Suguru Yonezawa.;Tsutomu Marui
• 通讯作者: Tsutomu Marui