Association with occult hepatitis C virus in the patients with non-alcoholic steatohepatitis

非酒精性脂肪性肝炎与隐匿性丙型肝炎病毒的相关性

• 批准号: 18590734
• 负责人: SATO Shuichi
• 金额: $ 2.09万
• 依托单位: Shimane University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590734/
• 关键词: Non alcoholic steatohenatitis; occult henatitis C virus; hepatocarcinogenesis; 滞在性HCV

In order to qualitatively and quantitatively detect the occult hepatitis C virus (HCV) in the patients with non-alcoholic steatohepatitis (NASH) , we underwent real-time PCR for HCV-RNA in the patients whose serum HCV RNA were positive (positive control, n=6) , with NASH whose serum HCV-RNA were negative (n=47) and the patients with other diseases example for benign tumor, metastatic liver tumor, drug-induced liver injury and cholangioma whose serum HCV-RNA were negative as well as NASH (negative control, n=14). lassay of real time PCR was necessary for 10 mg of liver tissue. The results in real time PCR were positive in all of the patients with chronic hepatitis C (4.7±6.5x10^5 copies/assay, mean±SD, range: 1.1-8.5x10^5 copies/assay. The viral load in 10mg of liver tissue estimated by Real time PCR significantly related to serum viral load in identified patient with hepatitis C (p<0.05) . However, HCV-RNA was not detected in the patients with NASH and other diseases whose serum HCV-RNA were negative. In addition, we demonstrated the real-time PCR for HCV-RNA using by resected liver specimen in the four patients with burned out NASH and hepatocellular carcinoma. As well as the patients with NASH, the results in real time PCR were negative in all of the patients. This results suggested that occult HCV did not associated with second hit theory for NASH.

为了在非酒精性脂肪性肝炎（NASH）患者中进行定性和定量检测丙型肝炎病毒（HCV），我们在血清HCV RNA的患者中接受了HCV-RNA的实时PCR，其血清HCV RNA为阳性（阳性对照，n = 6），其与NASH的患者，均与NASH患者进行了HCV-RNA的emirn and and and and and n = n = 47）转移性肝肿瘤，药物诱导的肝损伤和胆管瘤，其血清HCV-RNA为阴性和NASH（阴性对照，n = 14）。实时PCR的拉赛是10 mg肝组织所必需的。所有慢性丙型肝炎患者的实时PCR结果均为正（4.7±6.5x10^5副本/测定，平均值±SD，范围：1.1-8.5x10^5副本/测定。在10 mg的肝脏组织中，由10 mg的肝脏组织PCR估计的10 mg liver pcr估计的肝脏载荷与识别型患者（Hepatiity ccr）相关。在NASH和其他疾病中未检测到的血清HCV-RNA除此之外，我们还使用切除的HCV-RNA实时PCR使用了四个患者的NASH和肝细胞癌的肝脏中的肝脏标本。

项目成果

Anti-tumor effects of cimetidine on hepatocellular carcinomas in diethylnitrosamine-treated rats

西咪替丁对二乙基亚硝胺治疗大鼠肝细胞癌的抗肿瘤作用

• 发表时间: 2008
• 期刊: Oncology Reports 19
• 作者: Furuta K;et. al.
• 通讯作者: et. al.

Quantitative measurement of hepatitis B virus DNA in

乙型肝炎病毒DNA的定量测定

• 发表时间: 2006
• 期刊: Journal of Medical Virology 78
• 作者: Mishiro T;et al.
• 通讯作者: et al.

開けがわかる超音波ポケットアトラス

超声波袖珍阿特拉斯向您展示如何打开它

• 发表时间: 2007
• 作者: 木下芳一、佐藤秀一;他
• 通讯作者: 他

Ferucarbotran expands area treated by radiofrequency ablation in rabbit livers.

Ferucarbotran 扩大了兔肝脏射频消融治疗的面积。

• 发表时间: 2007
• 期刊: Journal of Gastroenterology and Hepatology 23(Epub)
• 作者: Miyake T;Sato S;et. al.
• 通讯作者: et. al.

Ferucarbotran expands area treated by radiofrequency ablation in rabbit livers

Ferucarbotran 扩大了兔肝脏射频消融治疗的面积

• 发表时间: 2007
• 期刊: Journal of Gastroenterology and Hepatology(Epub)
• 作者: Miyake;T.;Sato;S.;et. al.
• 通讯作者: et. al.