Development of probiotics which regulate bacterial quorum sensing

开发调节细菌群体感应的益生菌

基本信息

批准号：
18590437
负责人：
KAMIYA Shigeru
金额：
$ 2.57万
依托单位：
Kyorin University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590437/
关键词：
quorum sensing probiotics biofilm intestinal flora pathogenesis クオラムセンシン

项目摘要

Quorum sensing (QS) is a system for regulation of bacterial gene by autoinducer (AI) which is produced according to bacterial density. Bacterial biofilm is associated with immunological escape, resistance to anti-microbial agents, and its formation is regulated by QS. In order to develop the probiotics which regulate bacterial quorum sensing, the effect of intestinal flora bacteria on biofilm formation by E. coil was examined.Biofilm assay in microplate was established by using E. coll. Addition of AI-2 increased biofilm formation by E. coli in this assay. Using 16 species and 25 strains of intestinal flora bacteria, it was shown that culture supernatants of Bifidobacterium longm, Clostridium difficile, Enterococcus faecalis, Lactobacillus casei, Peptoniphilus asaccharolyticus and Veillonella parvula inhibited biofilm formation by E. coil. In contrast, culture supernatants of Bcateroides and Fusobacterium necrophorum enhanced biofilm formation. As there was no significant difference in biofilm formation between Helicobacter pylori TK1402 stain and its luxS mutant strain without AI-2 production, it was speculated that biofilm formation was not induced in IL pylori by QS.Using 576 strains isolated from feces of healthy adults, the effects of culture supernatants of these bacteria on biofilm formation by E. coli was examined. Twelve strains inhibited the biofilm formation with less than 80 % level of control. As biofilm formation by E. coli was not effectively done, the additional experiment using 534 stains was performed. Culture supernatants of 43 and 22 strains inhibited biofilm formation by E. coli by more than 20% and 50%, respectively. Culture supernatants of 42 and 5 strains inhibited biofilm formation by S. aureus by more than 205 and 50%, respectively. Identification of these strains and the effects of these stains on the expression of QS-related genes for adhesin and bacterial toxin need to be clarified.

法定人数传感（QS）是通过自动诱导剂（AI）调节细菌基因的系统，该系统是根据细菌密度产生的。细菌生物膜与免疫学逃生，对抗微生物剂的抗性有关，其形成受Q的调节。为了开发调节细菌群体传感的益生菌，检查了肠道菌群细菌对生物膜形成的影响。在该测定中，添加AI-2增加了大肠杆菌的生物膜形成。使用16种和25种肠道菌群细菌菌株，表明培养了长杆菌，艰难梭状芽胞杆菌，粪肠球菌，乳核酸杆菌，乳酸乳杆菌，乳酸乳杆菌，peptoniphilus asaccharolyticus as acaccharolyticus和veillonella parvula parvula parvula ribining Bibisitibilibing Bibising Bibising Bibising Bibibily by coil coil by coil coil coiltion。相比之下，BCATROIDES和梭杆菌的培养上清液增强了生物膜的形成。由于幽门螺杆菌幽门螺杆菌TK1402染色与其没有AI-2产生的LuxS突变菌株之间的生物膜形成没有显着差异，因此猜测，Q.通过576菌株从健康成年人中孤立的576菌株在IL幽门螺杆菌中诱导了生物膜的形成，这些菌株是由健康的成年人隔离的，这些培养成人的效果是由这些培养的超级氏菌的作用。十二菌株抑制了生物膜的形成，其对照水平少于80％。由于没有有效地由大肠杆菌形成生物膜，因此进行了534颗污渍的其他实验。 43和22菌株的培养上清液分别抑制大肠杆菌的生物膜形成超过20％和50％。 42和5菌株的培养上清液分别抑制了金黄色葡萄球菌的生物膜形成，分别超过205％和50％。需要鉴定这些菌株以及这些污渍对QS相关基因表达的粘附蛋白和细菌毒素的表达的影响。