Pathogenic role of heat shock protein of Helicobacter pylori and host immune response to the heat shock protein

幽门螺杆菌热休克蛋白的致病作用及宿主对热休克蛋白的免疫反应

• 批准号: 12670265
• 负责人: KAMIYA Shigeru
• 金额: $ 2.18万
• 依托单位: Kyorin University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670265/
• 关键词: H. pylori; heat shock protein; cytokine; vaccine; epitope

By using purified heat shock protein 60 (HSP60) of Helicobacter pylori and fusion protein of HSP60 with beta-galactosidase, it was shown that H. pylori HSP60 stimulated a secretion of interleukin-8 (IL-8) which is closely related with the induction of gastric mucosal inflammation from gastric epithelial cells. In addition, purified HSP60 induced apoptosis of gastric epithelial cells. These results indicate that HSP60 of H. pylori might play an important role in pathogenesis following H. pylori infection.An epitope reactable with monoclonal antibody (Mab) to HSP60 was detected on the surface of gastric epithelial cells. Immune response to H. pylori HSP60 was stronger in the sera of the patients with H. pylori infection than in those without H. pylori infection. Not only fusion protein of HSP60 with beta-galactosidase but also oligopeptide pH9 reactable with the Mab showed a protective effect on colonization of H. pylori in mice. These results suggest that HSP60 of H. pylori might be a good candidate as a component of vaccine

By using purified heat shock protein 60 (HSP60) of Helicobacter pylori and fusion protein of HSP60 with beta-galactosidase, it was shown that H. pylori HSP60 stimulated a secretion of interleukin-8 (IL-8) which is closely related with the induction of gastric mucosal inflammation from gastric epithelial cells.另外，纯化的HSP60诱导胃皮细胞凋亡。这些结果表明，幽门螺杆菌的Hsp60在幽门螺杆菌感染后可能在发病机理中起重要作用。在胃皮细胞表面检测到与HSP60的单克隆抗体（MAB）反应的表位。对幽门螺杆菌HSP60的免疫反应在幽门螺杆菌感染患者的血清中比没有幽门螺杆菌感染的患者更强。 HSP60的融合蛋白与β-半乳糖苷酶的融合蛋白，以及与MAB反应的寡肽PH9对小鼠幽门螺杆菌的定殖产生了保护作用。这些结果表明，幽门螺杆菌的Hsp60可能是疫苗的良好候选者

项目成果

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