MRI of the brain in a primate model of severe human malaria

严重人类疟疾灵长类动物模型的大脑 MRI

• 批准号: 18590404
• 负责人: KAWAI Satoru
• 金额: $ 2.57万
• 依托单位: Dokkyo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590404/
• 关键词: MRI; cerebral malaria; Plasmodium coatneyi; primate model; severe human malaria; マラリア疾患モデル

Our previous studies demonstrated that the Japanese macaque (Macaca fuscata) is highly susceptible to Plasmodium coatneyi infection and that pathologic findings in infected monkeys are similar to those observed in human falciparum malaria with cerebral involvement. To elucidate the clinical features of cerebral malaria and correlation with pathologic findings, we performed magnetic resonance imaging (MRI) analysis on the brain in our primate model. In the experiment, we prepared four J-macaques (J52, J53, J54 and J55), and they were examined by four times MR imaging before and after infection. After the 2nd imaging, the macaques were autopsied and processed for pathological examination. Parasitemia of J53 was 30.1% on day 14, and the macaque exhibited serious clinical signs such as restlessness and depression. However, MRI revealed no abnormalities. Histopathological examination showed preferential sequestration of PRBCs in the cerebral and cerebellum capillaries. However, parenchymal damages such as necroris and peri-vasculare edema were not found in the section. We therefore conclude that although sequestration in the microvessels was frequently seen in our primate model during acute malarial attack, the MRI abnormality of the brain is not common in our primate model during acute phase of severe malaria.

我们先前的研究表明，日本猕猴（Macaca fuscata）非常容易受到疟原虫感染的影响，并且感染猴子中的病理发现与在人恶性疟疾中观察到的猴子和脑受累。为了阐明脑疟疾的临床特征以及与病理发现的相关性，我们在灵长类动物模型中对大脑进行了磁共振成像（MRI）分析。在实验中，我们准备了四个J-Macaques（J52，J53，J54和J55），并在感染前后通过MR成像进行了四次检查。第二次成像后，猕猴被尸检并处理以进行病理检查。 J53的寄生虫病在第14天为30.1％，猕猴表现出严重的临床体征，例如不安和抑郁。但是，MRI没有发现异常。组织病理学检查表明，大脑和小脑毛细血管中PRBC的优先隔离。然而，本节中未发现实质性损害，例如死灵和血流旁水肿。因此，我们得出的结论是，尽管在急性疟疾攻击期间，在我们的灵长类动物模型中经常看到微血管中的隔离，但在严重疟疾的急性阶段，在我们的灵长类动物模型中，大脑的MRI异常并不常见。

项目成果

PbMP-1:植物とマラリア原虫との比較ゲノムによって同定されたマラリア原虫の受精制御因子

PbMP-1：通过植物和疟疾寄生虫的比较基因组鉴定的疟疾寄生虫的受精调节剂

• 发表时间: 2007
• 作者: 平井 誠;新井 明治;森 稔幸;川合 覚;北 潔;黒岩 常祥;松岡 裕之
• 通讯作者: 松岡 裕之

サル・マラリア疾患モデルを用いた新たな研究展開

使用猴子疟疾疾病模型的新研究进展

• 发表时间: 2007
• 作者: Kawai S;Saito N;Katakai Y;Ono F;Nakamura S;Yasutromi Y;Kamo S;川合 覚;川合 覚
• 通讯作者: 川合 覚

EDG-PET imaging in a primate model of severe human malaria Malaria

严重人类疟疾灵长类动物模型中的 EDG-PET 成像 疟疾

• 发表时间: 2007
• 期刊: In Columbus F(Ed)
• 作者: Kawai;S
• 通讯作者: S

Evolution and phylogeny of the heterogeneous cytosolic SSU rRNA genes in the genus Plasmodium

• DOI: 10.1016/j.ympev.2008.01.031
• 发表时间: 2008-04-01
• 期刊: MOLECULAR PHYLOGENETICS AND EVOLUTION
• 影响因子: 4.1
• 作者: Nishimoto, Yuriko;Arisue, Nobuko;Hashimoto, Tetsuo
• 通讯作者: Hashimoto, Tetsuo

磁気共鳴画像(MRI)を用いた実験的脳マラリアの病態解析

实验性脑型疟疾的磁共振成像（MRI）病理分析

• 发表时间: 2007
• 作者: 川合 覚;斉藤 直之;片貝 祐子;小野 文子;中村 紳一郎;揚山 直英;明里 宏文;寺尾 恵治
• 通讯作者: 寺尾 恵治