Central action of proteinacious hormones

蛋白质激素的中枢作用

基本信息

批准号：
18570063
负责人：
KIKUYAMA Sakae
金额：
$ 2.5万
依托单位：
Waseda University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

In the newt, Cynops pyrrhogaster,internal fertilization takes place in spite of the absence of copulatory organs. In order to transfer the spermatophore into the cloaca of the female, the male performs an unique courtship behavior. Our previous study revealed that besides androgen, prolactin(PRL) and arginine vasotocin(AVT) are involved in the expression of the courtship behavior and that both PRL and AVT act centrally to elicit the behavior .Immunohistochemical study using antibody against PRL receptor demonstrated that both the choroidal plexus and AVT containing neurons possess PRL receptors. Moreover pharmacological study using inhibitors of AVT receptors revealed that the action of AVT in terms of eliciting the behavior is mediated through Vla receptor, its distribution in the diencephalon being confined to the preoptic area, suprachiasmatic nucleus and ventral hypothalamus. This raised the possibility that PRL incorporated into the brain through the choroidal plexus acts on the AVT neurons and AVT mediates the action of PRL. To prove this hypothesis, experiments were conducted using androgen-primed male newts. Firstly it was confirmed that intracerebroventricular injection of antibody against PRL or antibody against PRL receptor blocked the expression of courtship behavior and that administration of AVT restored the expression of courtship behavior. On the other hand, the suppression of the behavior induced by the intracerebroventricular administration of an inhibitor of Vla receptor was never nullified by PRL supplementation. These results indicate that PRL primarily acts on the AVT neurons and AVT mediates the action of PRL to elicit the courtship behavior.

在Newt，Cynops pyrrhogaster中，尽管没有交配器官，但内部受精仍会发生。为了将精子运动转移到女性的泄殖腔中，男性执行独特的求爱行为。我们先前的研究表明，除了雄激素，催乳素（PRL）和精氨酸血管素（AVT）外，还参与了求爱行为的表达，并且PRL和AVT都集中于行为以引起这种行为。使用抗体对PRL受体的免疫组化研究表明，这两种抗体证明了这两种抗体证明了这两种行为脉络丛和含有神经元的AVT具有PRL受体。此外，使用AVT受体抑制剂的药理学研究表明，AVT在引发该行为的作用是通过VLA受体介导的，其在依次脑中的分布仅限于per骨前区域，上核核上核和腹侧下丘脑。这增加了PRL通过脉络丛掺入大脑的可能性作用于AVT神经元，并介导了PRL的作用。为了证明这一假设，使用雄激素培养的雄性NEW进行了实验。首先，可以证实，脑室内注射针对PRL的抗体或针对PRL受体的抗体阻止了求爱行为的表达，而AVT的给药恢复了求爱行为的表达。另一方面，从未通过补充PRL抑制VLA受体抑制剂的脑室内给药引起的行为抑制。这些结果表明，PRL主要作用于AVT神经元，并介导PRL引起求爱行为的行动。