Hormonal and pheromonal control of reproduction

生殖的激素和信息素控制

基本信息

批准号：
12440234
负责人：
KIKKUYAMA Sakae
金额：
$ 9.86万
依托单位：
Waseda university
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

项目摘要

Hormonal and pheromonal control of reproduction was investigated focusing on the hormones and pheromones involved in the expression of reproductive behavior in the newt, Cynopus pyrrhogaster. During the breeding season, the male newt exhibit a specific courtship behavior; vibration of the tail in front of the female partner discharge of a female-attracting pheromone (sodefrin) and deposition of spermatophores. The tail vibration was revealed to be induced by prolactin (PRL) and androgen. The expression of this behavior was enhanced by arginine vasotocin (AVT) via V la receptor. In this case, PRL and AVT were evidenced to act centrally. In the case of PRL, the site of action was postulated to be neurons situated in the preoptic recess organ and / or nucleus infundibularis dorsalis where immunoreactive PRL receptors are abundant AVT also induced the discharge of both sodefrin and spermatophore presumably by acting peripherally. Synthesis of sodefrin precursor protein in the abdominal gland of the cloaca was considered to be enhanced by PRL and androgen since sodefrin precursor mRNA levels were elevated by PRL and androgen. Responsiyeness of the vomeronasal epithelium of the female newt to sodefrin was also revealed to be under the control of PRL and estrogen as judged from electroolfactogram response. Sodefrin-precursor-like protein cDNA was obtained from a cDNA library of C. ensicauda abdominal gland. According to the deduced amino acid sequence of the protein, sodefrin-like pheromone of C. ensicauda was considered to be decapeptide with a substitution of two amino acid residues. This putative pheromone was isolated from the abdominal gland of C. ensicauda and presence of its bioactivity was confirmed. The pheromone, designated silefrin, was effective only on the conspesific female, as in the case of sodefrin, suggesting the newt pheromones are species-specifia.

研究了生殖的激素和信息素控制，重点是参与蝾螈（Cynopuspyrrhogaster）生殖行为表达的激素和信息素。在繁殖季节，雄性蝾螈会表现出特定的求偶行为；在雌性伴侣面前振动尾巴会释放出吸引雌性的信息素（sodefrin）并沉积精囊。尾部振动被揭示是由催乳素（PRL）和雄激素引起的。精氨酸加压素(AVT)通过V1a受体增强了这种行为的表达。在这种情况下，PRL 和 AVT 被证明发挥着中枢作用。就 PRL 而言，假定作用位点是位于视前隐窝器官和/或背漏斗核的神经元，其中免疫反应性 PRL 受体丰富，AVT 也可能通过外周作用诱导 sodefrin 和精囊的放电。由于 PRL 和雄激素提高了 sodefrin 前体 mRNA 水平，因此认为泄殖腔腹部腺中 sodefrin 前体蛋白的合成会被 PRL 和雄激素增强。从嗅电图反应判断，雌性蝾螈犁鼻上皮对 sodefrin 的反应性也受 PRL 和雌激素的控制。 Sodefrin-前体样蛋白 cDNA 是从 C. ensicauda 腹腺 cDNA 文库中获得的。根据推导的蛋白质氨基酸序列，认为C. ensicauda的sodefrin样信息素是由两个氨基酸残基取代的十肽。这种假定的信息素是从 C. ensicauda 的腹部腺中分离出来的，并证实了其生物活性的存在。这种信息素被命名为 silefrin，仅对雌性有效果，就像 sodefrin 的情况一样，这表明蝾螈信息素是物种特异性的。