Studies on development, structure and function of pituitary gland in amphibians

两栖类垂体发育、结构与功能的研究

• 批准号: 16370031
• 负责人: KIKUYAMA Sakae
• 金额: $ 8.45万
• 依托单位: Waseda University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16370031/
• 关键词: Hypophysis; Amphibian; Development; Structure and function; Reproductive behavior; Hypothalamus; 動物形態; 実験形態

1.Ontogenic study of pituitary glandAs a tool for tracing the cells that migrate with pituitary primordium but remain in the foregut region, transgenic toads (Xenopus laevis) that express GFP were prepared.2.Central action of prolactinAn adenohypophyseal hormone prolactin is involved in eliciting courtship behavior in the newt, genus Cynops, acting centrally. As a step to elucidate the mechanism of the prolactin action, distribution of prolactin receptors in the newt (Cynops pyrrhogaster) brain was studied by immunohistochemistry and in situ hybridization. Immunohistochemically detectable prolactin receptors as well as prolactin receptor mRNAs were observed in several region of hypothalamic nuclei, and most notably in the choroid plexus. Involvement of choroid plexus in transporting prolactin molecules into the brain was suggested.3.Regulation of pituitary function by hypothalamic neuropeptidesNewly developed radioimmunoassay for amphibian TSH made it possible to detect TSH-releasing factors in the amphibian brain. As a result, CRF was revealed to be the most potent substance to stimulate the release of TSH from the bullfrog pituitary. Using CRF antagonists and agonists, CRF receptor type 2 was demonstrated to mediate the action of CRF to release TSH.

1.垂体的个体发育研究作为追踪随垂体原基迁移但保留在前肠区域的细胞的工具，制备了表达 GFP 的转基因蟾蜍（非洲爪蟾）。 2.催乳素的中枢作用腺垂体激素催乳素参与诱发蝾螈属蝾螈的求偶行为中枢。作为阐明催乳素作用机制的一个步骤，通过免疫组织化学和原位杂交研究了蝾螈（Cynopspyrrhogaster）大脑中催乳素受体的分布。在下丘脑核的几个区域，尤其是在脉络丛中观察到免疫组织化学可检测的催乳素受体以及催乳素受体 mRNA。认为脉络丛参与了催乳素分子向大脑的转运。3.下丘脑神经肽对垂体功能的调节新开发的两栖动物TSH放射免疫分析方法使检测两栖动物脑内TSH释放因子成为可能。结果表明，CRF 是刺激牛蛙垂体释放 TSH 的最有效物质。使用 CRF 拮抗剂和激动剂，2 型 CRF 受体被证明可以介导 CRF 释放 TSH 的作用。

项目成果

In vitro effect of bisphenol A on the thyroid hormone receptor gene expression in the Xenopus tail and testis.

双酚A对非洲爪蟾尾部和睾丸甲状腺激素受体基因表达的体外影响。

• 发表时间: 2004
• 期刊: Trends in Comparative Endocrinology (T. Oishi, K. Tsutsui, S. Tanaka, S. Kikuyama (eds)) (Asia and Oceania Society for Comparative Endocrinology)
• 作者: S.Iwamuro;N.Aikawa;M.Yamada;S.Kikuyama
• 通讯作者: S.Kikuyama

Molecular cloning of bullfrog corticotropin-releasing factor (CRF) : effect of homologous CRF on the release of TSH from pituitary cells in vitro.

牛蛙促肾上腺皮质激素释放因子（CRF）的分子克隆：同源CRF对体外垂体细胞释放TSH的影响。

• 发表时间: 2004
• 期刊: Gen. Comp. Endocrinol. 138
• 作者: R.Urakawa;H.Toda;K.Haibara;Y.Ito et al.
• 通讯作者: Y.Ito et al.

Processing of multiple forms of preprosodefrin in the abdominal gland of the red-bellied newt Cynops pyrrhogaster : regional and individual differences in preprosodefrin.

红腹蝾螈腹腺中多种形式的前原索德林的加工：前原索德林的区域和个体差异。

• 发表时间: 2004
• 期刊: Peptides 25
• 作者: K.Iida;Y.Tang;T.Mukai;Y.Nishimori;T.Iwata et al.
• 通讯作者: T.Iwata et al.

Development of radioimmunoassay for bullfrog thyroid-stimulating hormone(TSH)

牛蛙促甲状腺激素（TSH）放射免疫分析方法的研制

• 发表时间: 2004
• 期刊: Gen.Comp.Endocrinol. 135
• 作者: Okada R;Yamamoto K;Kikuyama S;et al.
• 通讯作者: et al.

Prolactin acts centrally to enhance newt courtship behavior.

催乳素的主要作用是增强蝾螈的求偶行为。

• 发表时间: 2005
• 期刊: Gen. Comp. Endocrinol. 141
• 作者: Yamane;K.;Rahman;MdS.;Kawasaki;M.;Taniguchi;M.;Miyake;H.;F.Toyoda et al.
• 通讯作者: F.Toyoda et al.