The study of molecular and gene analysis and bone metabolism-improving effect of propiomibacterium freudenreichii milk whey culture against osteoporosis.

费氏丙酸杆菌乳清培养物抗骨质疏松的分子基因分析及骨代谢改善作用研究

• 批准号: 17390538
• 负责人: SUGAHARA Toshio
• 金额: $ 10.05万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17390538/
• 关键词: Osteoporosis; Bifidobacteria; Propiomibacterium freudenreichii; DHNA; T-cell; B-cell; Naphthoquinone; Bone mass recovery; 免疫抑制剤; T, B細胞; FACS

To see the relationship between immune cell and osteoporosis occurrence by immunosuppresant, we have established the osteoporosis mouse model using immunosuppresant (FK506) injected nude mouse and wild type mouse. The results of wild type were amount of trabecula bone under enchondral ossification was decreased, and number of osteoclast, TRAP positive, was obviously increased in enchondral ossification. On the other hand, there was almost no bone resorption under enchondral ossification in the nude mouse' s result. Those results suggest that immune cell contributes to the osteoporosis occurrence by the immunosuppresant. We have found propiomibacterium freudenreichii milk whey culture (PC), starter of Swiss cheese, could rescue osteoporosis in our model. In further experiment, PC could inhibit the differentiation of osteoclast and prevent osteoporosis. But it is hard to simply say that PC rescued osteoporosis in our model by differential inhibition of osteoclast, because of the osteocla … More st differentiation and immune system participation. To see the details, we have gotten the following results, a) The number of osteoclast was significantly increased in the mouse osteoblast-like stroma cell and osteoblast mixed culture with supplementation of CD4 positive T-cell or B220 positive B-cell, extracted from bone marrow with magnet beads. b) FACS result was the immunosuppresant decreased the number of T-cell and increased the number of B-cell in spleen and mesentericlymph node of our model, c) DHNA, contained in PC, has naphthoquinone. The basic skeleton is the same as vitamin K2, which is a research attention of bone mass recovery in recent years. DHNA made the same results as PC's and was identified as a factor of inhibitory effect of bone resorption.We elucidated the bone mass recovery with DHNA, and the relationship immune system and osteoclast. Furthermore, to identify osteoporosis-related gene, we have seen the alteration of gene expression in'bone marrow with DNA microarray. Less

为了查看免疫抑制剂发生的免疫细胞与骨质疏松症之间的关系，我们使用免疫磷酸盐（FK506）注入了裸鼠和野生型小鼠建立了骨质疏松小鼠模型。野生类型的结果是在链膜骨化下的小梁骨的量，改善了骨骨化骨化的破骨细胞的数量显然增加了。另一方面，在裸鼠的结果中，几乎没有骨骨化下的骨骼分辨率。这些结果表明，免疫细胞有助于免疫抑制剂发生的骨质疏松症。我们已经发现弗洛伊德里奇牛奶的丙型细菌为什么瑞士奶酪的培养物（PC）可以在我们的模型中挽救骨质疏松症。在进一步的实验中，PC可以抑制破骨细胞的分化并预防骨质疏松症。但是，很难简单地说，PC在我们的模型中反应了骨质疏松症，这是由于骨质骨化的差异抑制，因为骨质骨化……更多的ST分化和免疫系统的参与。为了查看细节，我们获得了以下结果，a）在小鼠成骨细胞样基质细胞和成骨细胞混合培养物中，骨质骨的数量显着增加，并补充了CD4阳性T细胞或B220阳性B细胞，该阳性B细胞从骨髓中用磁铁珠从骨髓中提取。 b）FACS结果是免疫补充剂改善了T细胞的数量，并增加了我们模型的脾和肠系膜lymph节点的B细胞数量，其中PC中包含的c）DHNA具有萘醌。基本骨骼与维生素K2相同，维生素K2是近年来骨骼质量恢复的研究。 DHNA与PC的结果相同，并被确定为骨恢复的抑制作用。我们阐明了使用DHNA的骨骼质量恢复，以及关系免疫系统和破骨细胞。此外，为了鉴定与骨质疏松相关的基因，我们看到了与DNA微阵列中基因表达的改变。较少的

项目成果

子どもは素晴らしい (お父さんとお母さんの子育てのために)

孩子们很精彩（因为爸爸妈妈的养育）

• 发表时间: 2006
• 作者: Shigeru;Maeda;Ichiro;Nakatsuka;Takuya;Miyawaki;Takuo;Kuboki;Masahiko;Shimada;Takashi Fujii;Tomohiro Yamada;Toshio Sugahara;Takaaki Ueno;Seiji Kondo;Seiji Kondo;Mishima K;Fujii T;Yamada T;Hitoshi Nagatsuka;Hitoshi Nagatsuka;菅原 利夫 共著;菅原 利夫 共著
• 通讯作者: 菅原 利夫 共著

子どもは素晴らしい(お父さんとお母さんの子育てのために)

孩子们很棒（对于养育孩子的爸爸妈妈来说）

• 发表时间: 2006
• 作者: Shigeru;Maeda;Ichiro;Nakatsuka;Takuya;Miyawaki;Takuo;Kuboki;Masahiko;Shimada;Takashi Fujii;Tomohiro Yamada;Toshio Sugahara;Takaaki Ueno;Seiji Kondo;Seiji Kondo;Mishima K;Fujii T;Yamada T;Hitoshi Nagatsuka;Hitoshi Nagatsuka;菅原 利夫 共著
• 通讯作者: 菅原 利夫 共著

Hypoxic Regulation of Stability of Connective Tissue Growth Factor/CCN2 mRNA by 3ユ-Untranslated Region Interacting with a Cellular Protein in Human Chondrosarcoma Cells.

3-非翻译区与人软骨肉瘤细胞中细胞蛋白相互作用对结缔组织生长因子/CCN2 mRNA 稳定性的缺氧调节。

• 发表时间: 2006
• 期刊: Oncogene 25
• 作者: Shigeru;Maeda;Ichiro;Nakatsuka;Takuya;Miyawaki;Takuo;Kuboki;Masahiko;Shimada;Takashi Fujii;Tomohiro Yamada;Toshio Sugahara;Takaaki Ueno;Seiji Kondo
• 通讯作者: Seiji Kondo

Expression of transcription factor Sox9 in the cartilage formation from grafted periosteal cells.

转录因子 Sox9 在移植骨膜细胞软骨形成中的表达。

• 发表时间: 2006
• 期刊: Asian Journal Oral Maxillofac 18 (1)
• 作者: Satoru;Sakurai;Atsuo;F.;Fukunaga,Takuya;Miyawaki;Tatsuya;Ichinohe;Yuzuru;KanekoCalifornia;USA;Takaaki Ueno
• 通讯作者: Takaaki Ueno

Novel angiogenic inhibitor DN-9693 that inhibits Past-transcriptional induction of connective tissue growth factor (CTGF/CCN2) by vascular endothelial grow factor (VEGF) in human endothelial cells.

新型血管生成抑制剂 DN-9693 可抑制人内皮细胞中血管内皮生长因子 (VEGF) 对结缔组织生长因子 (CTGF/CCN2) 的转录诱导。

• 发表时间: 2006
• 期刊: Molecular Cancer Therapeutics 5
• 作者: Shigeru;Maeda;Ichiro;Nakatsuka;Takuya;Miyawaki;Takuo;Kuboki;Masahiko;Shimada;Takashi Fujii;Tomohiro Yamada;Toshio Sugahara;Takaaki Ueno;Seiji Kondo;Seiji Kondo
• 通讯作者: Seiji Kondo