Molecule gene analysis of bone absorption in bone reproduction medical care and development of bone absorption restraint medicine

骨再生医疗中骨吸收的分子基因分析及骨吸收抑制药物的开发

• 批准号: 15390614
• 负责人: SUGAHARA Toshio
• 金额: $ 9.54万
• 依托单位: OKAYAMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15390614/
• 关键词: osteoprosis; osteoclast; Immunosuppresant agents; 免疫柳制剤; T細胞; RANKL; OPG; リアルタイムPCR; osteoprosis; osteoclast; Immunosuppresant agents; 免疫柳制剤; T細胞; RANKL; OPG; リアルタイムPCR

Intramuscular injection (1 mg/kg) did immunity restraint agent FK506 to a nude mouse and a mouse of a wild type that a T cell suffered a loss day after day on 28th and made an osteoporosis model mouse to search a cell pro-immunity and relevance with the onset of osteoporosis by immunity restraint agent. As a result, as for the bone absorption of a group of experiments by FK506, decrease of trabecula bone was recognized by a wild type more underneath than enchondral ossification, and, as for positive osteoclast, numbers increased in enchondral ossification for TRAP dyeing more clearly than a group of control again. However, as for the bone absorption in the lower part, as for the osteoporosis by immunity restraint agent, what an immunity system cell such as a T cell was related to was suggested without most than enchondral ossification with a nude mouse by there not being control and a change.I discovered that osteoporosis recovered when we administered Propion acid bacteria fermentatio … More n product (PC) that was a starter of Swiss cheese to this osteoporosis model. We examined a bone absorption restraint effect from this fermentation product this time. I was able to prevent the bone absorption when I administered Propion acid bacteria fermentation product (PC) to an osteoporosis mouse by FK506.In late years a lot of existing DHNA has a naphthoquinone frame the same as vitamins K2 attracting attention in a quantity of bone salt thing having quantity of bone improvement action within a PC.I paid attention to this point, and we accepted bone absorption restraint same as a PC for an osteoporosis model when we administered DHNA alone. As for DHNA, the bone absorption restraint effect of a PC was able to identify a thing by DHNA from what was included in a PC abundantly.I compared osteoclast with control when I added the B cell which was a T cell and the B220 positive that were four twist CD positive in marrow in osteoclast of a mouse namely in vitro of bone bud cell-like strike Rome cell and marrow cell with magnetic beads and increased significantly. Less

肌内注射（1 mg/kg）对裸鼠和野生型的小鼠进行了免疫震动剂FK506，T细胞日复一日地遭受了损失，并使骨质疏松型鼠标搜索了骨气造成的骨气免疫性，并与免疫疾病的骨质渗透剂的发作相关。结果，至于FK506通过FK506的一组实验的骨质损失，小梁骨的减少是通过在链接骨化的下方的野生型识别的，而对于阳性破骨细胞，链接骨化的数量增加了捕集诱捕的诱捕性染料的数量，而不是一组控制。然而，至于下部的骨骼遭受骨骼的痛苦，与免疫抗衡剂的骨质疏松症一样，建议没有大多数的免疫系统细胞（例如T细胞），而没有大多数与裸子用裸子骨化相关的，没有控制和变化。骨质疏松模型。这次我们检查了该发酵产品的骨损失约束作用。当我将丙酸细菌发酵产品（PC）施用到骨质疏松小鼠时，我能够防止骨骼损失，FK506在深近几年中，许多现有的DHNA具有萘季型框架，与维生素K2相同，与骨头损失的骨骼损失相同，在PC上占据了骨骼的骨骼损失，从而吸引了PC。骨质疏松模型时，当我们单独管理DHNA时。至于DHNA，PC的骨损失约束效应能够从PC中透露DHNA的一件事。当我添加B细胞时，将破骨细胞与对照组相比，它是T细胞和B220阳性的b220阳性，这些tw Twist CD是骨髓中的骨髓中的四个Twist CD阳性，它在骨纤维细胞中的骨细胞中均与骨骼般的细胞型细胞和型号beach型bex cy相比。较少的