Molecular characterization and role of ecdysone membrane receptor

蜕皮激素膜受体的分子特征和作用

• 批准号: 17380035
• 负责人: SAKURAI Sho
• 金额: $ 10.81万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17380035/
• 关键词: silk worm; Bombyx mori; programmed cell death; 20-hydroxyecdysone; sienal transduction; 膜受容体; 変態; 絹糸腺; カスパーゼ; カルシウム

We clarified developmental profiles of gene expression of early response genes to 20E in the anterior silk glands during the fifth instar up to the time of cell death execution two days after gut purge. Also, we showed the gene response to 20E in vitro using anterior silk glands of gut-purged larvae. The in vivo and in vitro results indicated that a heterodimeric EcR-B1 and USP-2 may be responsible for the cell death Results also indicated involvement of E74, E75, BHR3 and BR-C isoforms, but not Ftz-F1. We are not succeeded in gene cloning of the putative membrane ecdysone receptor yet. We examined pharmacologically the signaling pathway from mEcR to cellular responses, i.e. cell condensation, nuclear condensation, DNA fragmentation and nuclear fragmentation. Ca<2+> acts as the second messenger The mEcR is suggested to be a G-protein coupled receptor (GPCR) associated with Gαq, followed by a serial activation of phospholipase c-β, generation of inositol 3-phosphate (IP_3), and release of Ca<2+> from endoplasmic reticulum probably through IP3 receptor Then, Ca<2+> activates protein kinase C (PKC) and caspase 3-like protease. This signaling pathway culminates in nuclear fragmentation and nuclear fragmentation. Nuclear condensation is regulated by a different pathway involving calmodulin and calmodulin-dependent protein kinase II (CaMK-II). However, this pathway was not activated by Ca<2+>, and therefore it is unknown whether Gαq is involved in this pathway. In addition, inhibitors of calmodulin and CaMK-II affected the occurrence of nuclear and DNA fragmentations, indicating the caspase 3-like protease activation does not depend simply on the signaling pathway of GPCR/PLC-β/IP3/Ca<2+>/PKC.

我们阐明了早期呼吸基因在肠道清除后两天的时间内染色的前丝腺的20E的发育特征。体内和体外导致异二聚体ECR-B1和USP-2 Sults也表明E74，E75，BHR3和BR-C isforms的参与，但不是FTZ-F1。我们检查了从ME到细胞反应的信号通路，即细胞凝结，核凝结，DNA碎片碎片片段化。 ，肌醇3-磷酸盐（IP_3），然后通过IP3受体释放Ca <2 +>，然后通过IP3受体，Ca <2+>和caspase 3样蛋白酶释放。钙调蛋白和钙调蛋白依赖性蛋白激酶II（CAMK-II）。和DNA碎片，caspase 3样蛋白酶的激活取决于GPCR/PLC-β/IP3/Ca <2+>/PKC的ING途径。

项目成果

Identification, characterization, and developmental regulation of two storage proteins in the bamboo borer Omphisa fuscidentalis.

• DOI: 10.1016/j.jinsphys.2007.08.003
• 发表时间: 2008
• 期刊: Journal of insect physiology
• 影响因子: 2.2
• 作者: Jatuporn Tungjitwitayakul;T. Singtripop;Anchalee Nettagul;Y. Oda;Nujira Tatun;Takayuki Sekimoto;S. Sakurai

Nutritional status affects 20-hydroxyecdysone concentration and progression of oogenesis in Drosophila melanogaster.

• DOI: 10.1677/joe.1.06220
• 发表时间: 2005-10
• 期刊: The Journal of endocrinology
• 作者: J. Terashima;K. Takaki;S. Sakurai;M. Bownes

カイコ変態時に脳においてエクジソンにより誘導される遺伝子の網羅的解析-昆虫生理学的アプローチによる脳機能の解明.

蚕变态过程中大脑中蜕皮激素诱导基因的综合分析 - 使用昆虫生理学方法阐明大脑功能。

• 发表时间: 2005
• 作者: Sakiko;Shimizu;Monwar;Hossain;Yu;Kaneko;Sho;Sakurai;Hiroaki,Noda;Kazuei Mita;Masafumi;Iwami;松木正尋
• 通讯作者: 松木正尋

カイコガ幼虫脳におけるエクジソン応答遺伝子のマイクロアレイによる網羅的解析.

使用微阵列综合分析蚕幼虫大脑中的蜕皮激素反应基因。

• 发表时间: 2005
• 作者: Iga;M;清水咲子
• 通讯作者: 清水咲子

Spatial distribution of 20-hydroxyecdysone (20E)-responsive genes in the brain of silkworm, Bombyx mori.

家蚕 Bombyx mori 大脑中 20-羟基蜕皮激素 (20E) 反应基因的空间分布。

• 发表时间: 2007
• 作者: Roy;A.
• 通讯作者: A.