Molecular characterization and role of ecdysone membrane receptor

蜕皮激素膜受体的分子特征和作用

• 批准号: 17380035
• 负责人: SAKURAI Sho
• 金额: $ 10.81万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17380035/
• 关键词: silk worm; Bombyx mori; programmed cell death; 20-hydroxyecdysone; sienal transduction; 膜受容体; 変態; 絹糸腺; カスパーゼ; カルシウム

We clarified developmental profiles of gene expression of early response genes to 20E in the anterior silk glands during the fifth instar up to the time of cell death execution two days after gut purge. Also, we showed the gene response to 20E in vitro using anterior silk glands of gut-purged larvae. The in vivo and in vitro results indicated that a heterodimeric EcR-B1 and USP-2 may be responsible for the cell death Results also indicated involvement of E74, E75, BHR3 and BR-C isoforms, but not Ftz-F1. We are not succeeded in gene cloning of the putative membrane ecdysone receptor yet. We examined pharmacologically the signaling pathway from mEcR to cellular responses, i.e. cell condensation, nuclear condensation, DNA fragmentation and nuclear fragmentation. Ca<2+> acts as the second messenger The mEcR is suggested to be a G-protein coupled receptor (GPCR) associated with Gαq, followed by a serial activation of phospholipase c-β, generation of inositol 3-phosphate (IP_3), and release of Ca<2+> from endoplasmic reticulum probably through IP3 receptor Then, Ca<2+> activates protein kinase C (PKC) and caspase 3-like protease. This signaling pathway culminates in nuclear fragmentation and nuclear fragmentation. Nuclear condensation is regulated by a different pathway involving calmodulin and calmodulin-dependent protein kinase II (CaMK-II). However, this pathway was not activated by Ca<2+>, and therefore it is unknown whether Gαq is involved in this pathway. In addition, inhibitors of calmodulin and CaMK-II affected the occurrence of nuclear and DNA fragmentations, indicating the caspase 3-like protease activation does not depend simply on the signaling pathway of GPCR/PLC-β/IP3/Ca<2+>/PKC.

我们阐明了在肠道吹扫两天后的第五龄龄，直到细胞死亡执行时期，在第五龄型中开发了早期响应基因对20E的概况。另外，我们使用肠道幼虫的前丝腺在体外显示了对20E的基因反应。体内和体外结果表明，异二聚体ECR-B1和USP-2可能导致细胞死亡结果还表明E74，E75，BHR3和BR-C同工型的参与，而不是FTZ-F1。我们尚未成功地克隆假定的膜ecdysone受体。我们从MECR到细胞反应，即细胞凝结，核凝结，DNA碎片和核碎裂的药物信号传导途径。 Ca<2+> acts as the second messenger The mEcR is suggested to be a G-protein coupled receptor (GPCR) associated with Gαq, Followed by a serial activation of phospholipase c-β, generation of inositol 3-phosphate (IP_3), and release of Ca<2+> from endoplasmic reticulum probably through IP3 receptor Then, Ca<2+> activates protein kinase C （PKC）和caspase 3样蛋白。该信号通路最终导致核破碎和核破碎。核凝结受涉及钙调蛋白和钙调蛋白依赖性蛋白激酶II（CAMK-II）的不同途径调节。但是，该途径没有被Ca <2+>激活，因此尚不清楚GαQ是否参与该途径。此外，钙调蛋白和CAMK-II的抑制剂影响了核和DNA碎片的发生，表明caspase 3类蛋白酶激活不仅取决于GPCR/PLC-β/PLC-β/ip3/ca <2+>/pkc的信号传导途径。

项目成果

Identification, characterization, and developmental regulation of two storage proteins in the bamboo borer Omphisa fuscidentalis.

• DOI: 10.1016/j.jinsphys.2007.08.003
• 发表时间: 2008
• 期刊: Journal of insect physiology
• 影响因子: 2.2
• 作者: Jatuporn Tungjitwitayakul;T. Singtripop;Anchalee Nettagul;Y. Oda;Nujira Tatun;Takayuki Sekimoto;S. Sakurai

Nutritional status affects 20-hydroxyecdysone concentration and progression of oogenesis in Drosophila melanogaster.

• DOI: 10.1677/joe.1.06220
• 发表时间: 2005-10
• 期刊: The Journal of endocrinology
• 作者: J. Terashima;K. Takaki;S. Sakurai;M. Bownes

カイコ変態時に脳においてエクジソンにより誘導される遺伝子の網羅的解析-昆虫生理学的アプローチによる脳機能の解明.

蚕变态过程中大脑中蜕皮激素诱导基因的综合分析 - 使用昆虫生理学方法阐明大脑功能。

• 发表时间: 2005
• 作者: Sakiko;Shimizu;Monwar;Hossain;Yu;Kaneko;Sho;Sakurai;Hiroaki,Noda;Kazuei Mita;Masafumi;Iwami;松木正尋
• 通讯作者: 松木正尋

カイコガ幼虫脳におけるエクジソン応答遺伝子のマイクロアレイによる網羅的解析.

使用微阵列综合分析蚕幼虫大脑中的蜕皮激素反应基因。

• 发表时间: 2005
• 作者: Iga;M;清水咲子
• 通讯作者: 清水咲子

Spatial distribution of 20-hydroxyecdysone (20E)-responsive genes in the brain of silkworm, Bombyx mori.

家蚕 Bombyx mori 大脑中 20-羟基蜕皮激素 (20E) 反应基因的空间分布。

• 发表时间: 2007
• 作者: Roy;A.
• 通讯作者: A.