MOLECULAR MECHANISMS OF PROGRAMMED CELL DEATH BY ECDYSTEROID IN BOMBYX ANTERIOR SILK GLAND

家蚕前丝腺脱皮类固醇程序性细胞死亡的分子机制

• 批准号: 14360033
• 负责人: SAKURAI Sho
• 金额: $ 9.28万
• 依托单位: KANAZAWA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14360033/
• 关键词: ecdysteroid; silkworm; anterior silk gland; programmed cell death; steroid hormone; membrane receptor; non-genomic action; 絹糸腺; 絹系腺; 細胞死; アポトーシス; ステロイド

Programmed cell death (PCD) of anterior silk glands of the silkworm, Bombyx mori, is induced by 20E elicits its effects via binding with a heterodimeric nuclear receptor (EcR/USP) and acting as a transcriptional factor. In the PCD, however, we found various events associated with the cell death, which cannot be interpreted from the point of view of 20E action via nuclear receptor, and therefore we assumed the presence of a non-genomic action of 20E via ecdysone membrane receptor (mEcR). We confirmed the presence of the binding sites of ecdysteroids on membrane proteins. The responsible protein is embedded in plasma membrane and possesses a high binding activity with ecdysteroids. In addition, we found that 20E increases an intracellular cAMP level as rapidly as 30 seconds after 20E challenge. We also demonstrated that activation of protein kinase C and caspase 3 is involved in the 20E-induced PCD. The PCD precedes via plasma membrane blebbing, cellular shrinkage, DNA oligonucleosomal fragmentation, nuclear condensation, nuclear fragmentation and apoptotic body formation. Blebbing, cell shrinkage and apoptotic body formation are under the control of genomic action of 20E while other events are under the non-genomic action of 20E, probably through mEcR. The present results suggest that a single steroid hormone exerts its physiological effects through both genomic and nongenomic pathways.

家蚕前丝腺的程序性细胞死亡 (PCD) 是由 20E 诱导的，通过与异二聚体核受体 (EcR/USP) 结合并充当转录因子，从而引发其效应。然而，在PCD中，我们发现了与细胞死亡相关的各种事件，这不能从20E通过核受体作用的角度来解释，因此我们假设存在20E通过蜕皮激素膜受体的非基因组作用(mECR)。我们证实了膜蛋白上蜕皮类固醇结合位点的存在。负责的蛋白质嵌入质膜中并具有与蜕皮类固醇的高结合活性。此外，我们发现20E激发后30秒内细胞内cAMP水平迅速增加。我们还证明了蛋白激酶 C 和 caspase 3 的激活参与了 20E 诱导的 PCD。 PCD 通过质膜起泡、细胞收缩、DNA 寡核小体断裂、核浓缩、核断裂和凋亡体形成而发生。起泡、细胞皱缩和凋亡小体形成均受 20E 基因组作用的控制，而其他事件则受 20E 的非基因组作用（可能通过 mEcR）控制。目前的结果表明，单一类固醇激素通过基因组和非基因组途径发挥其生理作用。

项目成果

Death commitment in the anterior silk gland of the silkworm, Bombyx mori

• DOI: 10.1016/j.jinsphys.2004.10.012
• 发表时间: 2005-01-01
• 期刊: JOURNAL OF INSECT PHYSIOLOGY
• 影响因子: 2.2
• 作者: Kakei, M;Iwami, M;Sakurai, S
• 通讯作者: Sakurai, S

Presence of membrane ecdysone receptor in the anterior silk gland of the silkworm Bombyx mori

• DOI: 10.1111/j.1432-1033.2004.04249.x
• 发表时间: 2004-08-01
• 期刊: EUROPEAN JOURNAL OF BIOCHEMISTRY
• 作者: Elmogy, M;Iwami, M;Sakurai, S
• 通讯作者: Sakurai, S

Koyama, T., Obara, Y., Iwami, M., Sakurai, S.: "Commencement of pupal commitment in late penultimate instar and its hormonal control in wing imaginal discs of the silkworm"J.Insect Physiol.. (in press).

Koyama, T.、Obara, Y.、Iwami, M.、Sakurai, S.：“倒数第二龄晚期蛹的开始及其对蚕翅成虫盘的激素控制”J.Insect Physiol..（出版中）

Takaki, K., Sakurai, S.: "Regulation of prothoracic gland ecdysteroidogenesis activity leading to pupal metamorphosis"Insect Biochem.Mol.Biol.. 33. 1189-1199 (2003)

Takaki, K., Sakurai, S.：“调节前胸腺蜕皮类固醇生成活性导致蛹变态”Insect Biochem.Mol.Biol.. 33. 1189-1199 (2003)

Regulation of prothoracic gland ecdysteroidogenesis activity leading to pupal metamorphosis.

调节前胸腺蜕皮类固醇生成活性导致蛹变态。

• 发表时间: 2003
• 期刊: Insect Biochem.Mol.Biol. 33
• 作者: Takaki;K.;Sakurai;S.
• 通讯作者: S.