Molecular biologic and pathologic analysis of Epstein-Barr virus infection related salivary lymphoepithelial carcinoma

EB病毒感染相关唾液淋巴上皮癌的分子生物学和病理学分析

• 批准号: 16406033
• 负责人: CHENG Jun
• 金额: $ 7.23万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16406033/
• 关键词: lymphoepithelial carcinoma; salivary gland; EBV; LMP1 gene; cell culture; cloning; lymphoepithelial cyst; benign lymphoepithelial lesion; リンパ上皮性癌; 中国; 遺伝子導入; 塩基配列決定; NF-kB

We have collected the total 180 cases of lymphoepithelial carcinoma (LEC) of salivary gland, which were confirmed as EBV-infected tumors by various molecular biological.A complete sequence for the LMP1 gene, one of the EBV gene and has been regarded as an oncogene, was obtained from patients' tissue samples. It was cloned into an expression vector and the constructs were transfected into 293T cells. The transfected cells showed significant elevation of NFkB activities and suppressed cell growth, which were also seen in cells transfected with vectors with different LMP1 genes such as CAO, which has been isolated from a nasopharyngeal carcinoma, and B95-8, a prototype EBV. The results indicated that the expression of LMP1 affects transcription activities in LEC cells but that the overall mutations found in the present study were not always reasons for the tumorigenesis. The promoter region of the LMP1 gene was also analyzed from 20 cases of them, and all of the cases shared common mutational events in regions that were expected to be associated with transcription factors.We tried primary cultures for salivary LECs for many times. Unfortunately, however, it was hard to establish cell lines from the cells in culture. In one of the primary cultures, we were successful in maintaining cells, which were confirmed to be their salivary duct epithelial and myoepithelial characteristics as well as EBV infected.As a background disease, lymphoepithelial cyst was studied based on clinicopathological analyses of sixty-four cases of the parotid gland. The lymphoid stroma of the cyst seemed to be resulted from granulation tissue reaction of focal sialadenitis but not induced by EBV infection.Our functional study shows a new insight for EBV roles in the pathogenesis of salivary LEC, although it is still necessary to carry out further investigations for the whole mechanisms of EBV oncogenesis.

我们收集了180例唾液腺淋巴上皮癌（LEC），经多种分子生物学证实为EBV感染肿瘤。EBV基因之一的LMP1基因的完整序列，已被视为癌基因，是从患者的组织样本中获得的。将其克隆到表达载体中，并将​​构建体转染到 293T 细胞中。转染的细胞表现出 NFkB 活性显着升高并抑制细胞生长，这在用不同 LMP1 基因的载体转染的细胞中也可见到，例如从鼻咽癌中分离出的 CAO 和原型 EBV B95-8。结果表明，LMP1的表达影响LEC细胞的转录活性，但本研究中发现的总体突变并不总是肿瘤发生的原因。还对其中20例的LMP1基因启动子区域进行了分析，所有病例在预期与转录因子相关的区域中都有共同的突变事件。我们多次尝试唾液LECs的原代培养。然而不幸的是，很难从培养的细胞中建立细胞系。在其中一个原代培养物中，我们成功地维持了细胞，证实其具有唾液管上皮和肌上皮特征以及EBV感染。淋巴上皮囊肿作为一种背景疾病，通过对64例病例的临床病理分析进行了研究。腮腺的。囊肿的淋巴基质似乎是由局灶性唾液腺炎的肉芽组织反应引起的，而不是由EBV感染引起的。我们的功能研究为EBV在唾液腺LEC发病机制中的作用提供了新的见解，但仍有必要进一步开展研究 EBV 肿瘤发生的整个机制。

项目成果

A clinicopathologic study of parotid gland lymphoepithelial cyst

腮腺淋巴上皮囊肿的临床病理学研究

• 发表时间: 2006
• 期刊: Oral Med Pathol 11(4)
• 作者: Izumi Y;Uchida E;Sunyoto and Iijima M;Wu L
• 通讯作者: Wu L

口蓋腫瘍

腭肿瘤

• 发表时间: 2013
• 作者: 程 クン;丸山 智;阿部達也, 山崎 学;朔 敬
• 通讯作者: 朔 敬

Epstein-Barr virus infection in benign lymphoepithelial lesions with malignant transformation of salivary glands

EB病毒感染导致良性淋巴上皮病变伴唾液腺恶变

• 发表时间: 2004
• 期刊: Zhonghua Kou Qiang Yi Xue Za Zhi 39(4)
• 作者: Wu LY;Cheng J;Lu Y;Zhou ZY;Saku T
• 通讯作者: Saku T

Epstein-Barr virus infection in lymphoepithelial carcinoma of salivary glands in Sichuan Chinese

四川省唾液腺淋巴上皮癌中 Epstein-Barr 病毒感染

• 发表时间: 2004
• 期刊: Sichuan Da Xue Xue Bao Yi Xue Ban 35(4)
• 作者: Izumi Y;Uchida K;Iijima M;Wu LY
• 通讯作者: Wu LY

Primary cultures of Epstein-Barr virus-related lymphoepithelial carcinoma cells of the salivary gland

唾液腺 Epstein-Barr 病毒相关淋巴上皮癌细胞的原代培养

• 发表时间: 2005
• 期刊: Korean J Oral Maxillofacial Pathol 29(2)
• 作者: Cheng J;Jen KY;Zhou X;Tian Z;Maruyama S;Li J;Saku
• 通讯作者: Saku