Metastasis-associated genes in salivary adenoid cystic carcinoma and oral squamous cell carcinoma : a differential DNA chip analysis between metastatic and non-metastatic cell systems

唾液腺腺样囊性癌和口腔鳞状细胞癌的转移相关基因：转移性和非转移性细胞系统之间的差异DNA芯片分析

• 批准号: 14571728
• 负责人: CHENG Jun
• 金额: $ 1.92万
• 依托单位: NIIGATA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571728/
• 关键词: salivary gland tumor; p53; cancer suppressor gene; gene mutation; DNA; PCR; sequencing; SSCP; 細胞外基質分子

Overall modes of differential gene expressions were analyzed between human oral/salivary carcinoma cell systems with(MK-1 and ACCM) and without(ZK-1/ZK-2 and ACC2/ACC3) metastatic potential by using micro-array analysis with cancer-associated DNA chips, to determine what kinds of genes were associated with metastatic behaviors. Conspicuous differences in the level of mRNA expression of genes, which are responsible for, cell growth, apoptosis, cytoskeletons, extracellular matrix molecules metabolism, and cellular attachment were commonly shared between ACC2/ACC3 and ACCM or between ZK-1/ZK-2 and MK-1. ACCM and/or MK-1,which have highly metastatic potential, showed lower levels of gene expression in extracellular matrix-related molecules, such as collagens type IV/XVII and laminin, such adhesion molecules as integrins alpha 2/3, cadherin 2,thrombospondin 1/2 and CD44 antigen, but higher levels of genes controlling extracellular matrix degradation, such as MMP 9/15, and cell growth and cell cycle, such as FGF3,FGF7,Sjogren's syndrome/scleroderma autoantigen 1, cyclin dependent kinase 4 and cyclin D1 and adenine phosphoribosyltransferase. The higher protein expression levels for FGF7 in ACCM and lower protein levels for Collagen type IV and laminin were conformed by immunofluorescence. Metastatic potentials of oral/salivary carcinoma cells seem to be resulted from some combinations of over-/under-expression of the genes, which are responsible particularly for cell growth and extracellular matrix metabolism.

通过使用癌症微阵列分析，分析了具有（MK-1和ACCM）和不具有（ZK-1/ZK-2和ACC2/ACC3）转移潜力的人类口腔/唾液癌细胞系统之间差异基因表达的总体模式相关的 DNA 芯片，以确定哪些基因与转移行为相关。负责细胞生长、凋亡、细胞骨架、细胞外基质分子代谢和细胞附着的基因 mRNA 表达水平在 ACC2/ACC3 和 ACCM 之间或 ZK-1/ZK-2 和MK-1。具有高度转移潜力的 ACCM 和/或 MK-1 在细胞外基质相关分子中表现出较低水平的基因表达，例如 IV/XVII 型胶原和层粘连蛋白，例如整合素 α 2/3、钙粘蛋白 2、血小板反应蛋白 1/2 和 CD44 抗原，但控制细胞外基质降解的基因水平较高，例如 MMP 9/15，以及细胞生长和细胞周期，例如FGF3、FGF7、干燥综合征/硬皮病自身抗原 1、细胞周期蛋白依赖性激酶 4 和细胞周期蛋白 D1 和腺嘌呤磷酸核糖基转移酶。免疫荧光证实 ACCM 中 FGF7 蛋白表达水平较高，IV 型胶原蛋白和层粘连蛋白蛋白表达水平较低。口腔/唾液癌细胞的转移潜力似乎是由基因过度/不足表达的某些组合引起的，这些基因特别负责细胞生长和细胞外基质代谢。

项目成果

Ito M, Izumi N, Cheng J, Sakai H, Shingaki S, Nakajima T, Oda k, Saku T: "Jaw bone remodeling at the invasion front of gingival squamous cell carcinomas"J Oral Pathol Med. 32(1). 10-17 (2003)

Ito M、Izumi N、Cheng J、Sakai H、Shingaki S、Nakajima T、Oda k、Saku T：“牙龈鳞状细胞癌侵袭前部的颌骨重塑”J Oral Pathol Med。

Jen K Y: "Mutational events in LMP gene of Epstein-Barr virus in salivary gland lymphepithelial carcinomas"Int J Cancer. (in press). (2003)

Jen KY：“唾液腺淋巴上皮癌中 Epstein-Barr 病毒 LMP 基因的突变事件”Int J Cancer。

Maruyama S: "Metastasis-associated genes in oral and salivary carcinoma cells : A DNA chip analysis"J Oral Pathol Med. 31(5). 302 (2002)

Maruyama S：“口腔和唾液癌细胞中的转移相关基因：DNA 芯片分析”J Oral Pathol Med。

Maruyama S: "Metastasis-associated genes in oral and salivary carcinoma cells : A DMA chip analysis"J Oral Pathol Med. 31(5). 302 (2002)

Maruyama S：“口腔和唾液癌细胞中的转移相关基因：DMA 芯片分析”J Oral Pathol Med。

Ito M: "Jaw bone remordeling at the invasion front of gingival squamous cell carcinomas"J Oral Pathol Med. 32(1). 10-17 (2003)

Ito M：“牙龈鳞状细胞癌侵袭前部的颌骨重塑”J Oral Pathol Med。