Studies on emerging diseases derived from Primates and Chiroptera

源自灵长类动物和翼手目动物的新发疾病的研究

• 批准号: 16405036
• 负责人: AKASHI Hiroomi
• 金额: $ 8.51万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16405036/
• 关键词: Eboravirus; ELISA; epidemiological survey; immunofluorescent; anti-bat IgG rabbit serum; bat immunoglobulin; B virus; Yokose virus; ELESA法

1. Research on Eboravirus and B virusImmunofluorescent technique using HeLa cells expressing Eboravirus nucleocapsid protein and, ELISA method with baculovirus expressed Eboravirus antigen were established to detect antibody against the virus from primates. We also established fluorometric ELISA using expressed B virus gD protein. This method could detect specific antibody against B virus from primates with no cross reactivity to human herpesviruses.2. Research on bat immune systemAnti-bat IgG rabbit serum was produced to establish conventional ELISA method for the detection of viral antibody from bat sera. This anti-bat IgG rabbit serum reacted with IgG of Mega and Microbats tested. Moreover, we determined the nucleotide sequences of the bat CD4, IgFcRn, IFN-α subtype 1, and INF-β coding region.3. Serological survey of bat virusesThe labeled avidin-biotin ELISA system was established to detect antibody against Yokose virus from bats. To examine the availability of this ELISA, bat serum samples collected in the Philippines and Malaysia was conducted. 2.7% of the samples from the Philippines and 19% from Malaysia had detectable antibodies. We also conducted experimental infection of Yokose virus to frugivorous bats. The results showed poor replication of the virus in bats, suggesting that bats do not seem to serve as an amplifying host for Yokose virus.

1。使用表达Eboravirus核ocapsid蛋白的HeLa细胞的Eboravirus和B病毒荧光荧光技术的研究，以及用杆状病毒表达的ELISA方法的ELISA方法建立了Eboravirus抗原，以检测抗隐私病毒的抗体。我们还使用表达的B病毒GD蛋白建立了荧光测量ELISA。该方法可以从隐私中检测到针对B病毒的特异性抗体，而对人疱疹病毒没有交叉反应。2。对BAT Immuno Systemanti-Bat IgG兔血清的研究进行了研究，以建立传统的ELISA方法，用于检测BAT Sera的病毒抗体。这种抗BAT IgG兔血清与测试的Mega和Microbats IgG反应。此外，我们确定了BAT CD4，IGFCRN，IFN-α亚型1和INF-β编码区的核乙序序列3。建立了标记为Avidin-Biotin ELISA系统的BAT病毒的血清学调查，以检测蝙蝠对Yokose病毒的抗体。为了检查这种ELISA的可用性，进行了在菲律宾和马来西亚收集的BAT血清样品。菲律宾的样本中有2.7％，马来西亚的19％发现了抗体。我们还对烤蝙蝠进行了Yokose病毒的实验感染。结果表明，蝙蝠中病毒的复制不佳，表明蝙蝠似乎并没有作为Yokose病毒的放大宿主。

项目成果

翼手目の特性について

关于翼手目的特征

• 发表时间: 2006
• 期刊: 獣医畜産新報 59
• 作者: 大松勉;渡辺俊平;西村順裕;石井寿幸;朴銀庭;寺尾恵治;遠矢幸伸;久和茂;明石博臣;吉川泰弘
• 通讯作者: 吉川泰弘

Characteristics of Chiroptera

翼手目的特征

• 发表时间: 2006
• 期刊: Jyuichikusanshinpo 59
• 作者: Omatsu T;Watanabe S;Nishimura Y;Ishii Y;Bak EJ;Terao K;Tohya Y;Kyuwa S;Akashi H;Yoshikawa Y
• 通讯作者: Yoshikawa Y