Studies on atypical cutaneous leishmaniasis in Sri Lanka

斯里兰卡非典型皮肤利什曼病研究

• 批准号: 16406007
• 负责人: MATSUMOTO Yoshitsugu
• 金额: $ 6.4万
• 依托单位: The Universiyt of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16406007/
• 关键词: Sri Lanka; Leishmaniasis; Cutaneous leishmaniasis; Sand fly; リーシュマニア種

Although leishmaniasis is not considered to be epidemic in Sri Lanka, cutaneous leishmaniasis cases were confirmed by NNN culture and/or Giemsa-stained smears in 2003. The skin lesions of these cutaneous leishmaniasis patients were generally a few in number, and were small, blister in appearance, non-ulcerative, even lesions lasted 1.5 to 36 months. To identify pathogenic species, the nucleotide sequences of a part of the actin-encoding gene which is highly conserved among eukaryotes, the mini-exon gene which is unique in the genus Leishmania, and cysteine protease B gene which is useful for L.infantumlL. donovani discrimination were analyzed using isolated parasites. Interestingly, the comparison of the nucleotide sequences of Sri Lanka isolates with those of reference strains of Leishmania spp. showed the highest homology with that of L.donovani s.1., a principal pathogenic species of visceral leishmaniasis. Kalazar detect dipstick for visceral leishmaniasis, which is antibody detect … More ion assay to rK39 antigen and highly sensitive for visceral leishmaniasis caused by L.donovani s.1. was done and all patients were negative. The results confirm that the prevalent dermotropic parasite in Sri Lanka belongs to the L. donovani s.1. In addition, the sand fly fauna of Sri Lanka was investigated. Although, the identification of vector sand flies is important for the planning of control against this disease in any given endemic site, it has not been achieved yet in Sri Lanka. Five hundred and eighty four sand flies were collected in total. Identification of sand flies was performed based on morphological features. P.argentipes were widely distributed in the island whereas P.stantoni were collected only in Golle district. Since P.argentipes is know to be the vector of L. donovani causing visceral leishmaniasis in India, P.argentipes might be the most possible candidate vector of human cutaneous leishmaniasis in Sri Lanka. The present study described at the first time an endemic of cutaneous leishmaniasis caused by L.donovani s.1.. Less

尽管在斯里兰卡不认为利什曼病被认为是流行病，但2003年，nnn培养和/或giemsa染色的涂片证实了皮肤利什曼病病例。这些皮肤利什曼病患者的皮肤病变通常是少数数量，而且是小小的水泡，非尿液，甚至持续的liss，甚至持续了1.5 to。为了鉴定致病物种，在真核生物中高度保守的肌动蛋白编码基因的核苷酸序列，在利什曼原虫属中是独一无二的微型exon基因和半胱氨酸蛋白酶b基因，可用于l.infantumll。使用分离的寄生虫分析了Donovani歧视。有趣的是，Sri Lanka分离株的核苷酸序列与Leishmania spp的参考菌株的比较。与L.Donovani S.1。的同源性最高，这是内脏利什曼病的主要致病物种。 Kalazar检测插件用于内脏利什曼病，这是抗体检测的……更多的离子测定对RK39抗原，并且对由L. donovani S.1引起的内脏利什曼病高度敏感。已经完成，所有患者均为阴性。结果证实，斯里兰卡的普遍皮肤寄生虫属于多诺瓦尼乳杆菌。此外，研究了斯里兰卡的沙蝇动物区系。虽然，鉴定矢量沙蝇对于在任何给定的内在部位的控制这种疾病的控制都很重要，但在斯里兰卡尚未实现。总共收集了五百四十四个沙蝇。根据形态特征对沙蝇进行识别。 P.argentipes广泛分布在该岛，而P.Stantoni仅在Golle地区收集。由于已知P. argentipes是在印度引起内脏利什曼病的多诺万氏乳杆菌的载体，因此，p.argentipes可能是斯里兰卡人类皮肤利什曼病的最可能的候选媒介。本研究首次描述了由L.Donovani S.1引起的皮肤利什曼病的内在。

项目成果

スリランカにおけるLeishmania donovani s.1.による皮膚型リーシュマニア症の保虫宿主の血清学的探索

斯里兰卡杜氏利什曼原虫 s.1 引起的皮肤利什曼病储存宿主的血清学搜索。

• 发表时间: 2008
• 期刊: 獣医寄生虫学雑誌 6・2
• 作者: Akira Haraguchi;Tsutomu Iyobe;麻田正仁
• 通讯作者: 麻田正仁

環境改変による感染症流行の危機-沙漠緑化と感染症の増加-

环境变化导致传染病流行的风险 - 沙漠绿化和传染病增加 -

• 发表时间: 2005
• 期刊: 地球環境 10(1)
• 作者: 松本芳嗣;三條場千寿
• 通讯作者: 三條場千寿

スリランカにおけるLeishmania donovani s.l.による皮膚型リーシュマニア症の保虫宿主の血清学的探索

斯里兰卡杜氏利什曼原虫引起的皮肤利什曼病储存宿主的血清学研究

• 发表时间: 2007
• 作者: Matsueda;H.;平良文亨;渡辺寧;麻田正仁
• 通讯作者: 麻田正仁

Ciliatamides A-C, Bioactive Lipopeptide from the Deep-Sea Sponge Aaptos ciliata

Ciliatamides A-C，来自深海海绵 Aaptos ciliata 的生物活性脂肽

• 发表时间: 2008
• 期刊: Journal of Natural Products 71
• 作者: Akira Haraguchi;Tsutomu Iyobe;麻田正仁;Yasuyuki Goto;Yoichi Nakao
• 通讯作者: Yoichi Nakao

Serological tests for reservoir host surveillance on human cutaneous leishmaniasis caused by Leishmania donovani s.l. in Sri Lanka

杜氏利什曼原虫引起的人类皮肤利什曼病的储存宿主监测血清学试验

• 发表时间: 2008
• 期刊: Japanese journal of veterinary parasitology 6-2
• 作者: Akira Haraguchi;Tsutomu Iyobe;麻田正仁;Yasuyuki Goto;Yoichi Nakao;Masahito Asada
• 通讯作者: Masahito Asada