Epidemiology and Control of Leishmaniasis in Cetral Eurasia ; A new epidemic in association with irrigation of deserts

欧亚大陆中部利什曼病的流行病学和控制；

• 批准号: 08041169
• 负责人: MATSUMOTO Yoshitsugu
• 金额: $ 8.38万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08041169/
• 关键词: Leishmaniasis; Central Eurasia; irrigation of deserts; Great gerbils; Zoonosis; Pathogenic protozoa; Molecular Epidemiology; Arthropod-borne Infection; 中華人民共和国; 中央アジア

An epidmiological research was undertaken in this year in Xinjinang Uyger Autonomous Region of the People's Republic of China. Twelve Great gerbils (Rhombomys opimus) were captured by traps, 101 female sandflies were collected from their burrows. Stamp smears were made from auricula, liver, and spleen of captured great gerbils, fixed with methanol, and stained with Giemsa. A part of the auricula was fixed with 10% buffered formalin and embeded in paraffin wax. Sections were made and stained with hematoxylin and eosin. Sandflies were dissected and their guts were examined microscopically for the presence of promastigotes. Sandflies guts in ehich promastigotes were obtained were transferred to tubes of NNN medium in order to isolate the parasites. As a result, six Leishmania isolates were obtained from three species of sandflies. Three isolates of Leishmania parasites were established from the auricula of the three gerbils. In histopathological examination of naturalli infected great ger … More bils,amastigotes were observed in the auricula but not in spleen and liver. No significant pathological changes were obtained. Eight inbred BALB/c mice and eight Mongolian gerbils were subcutaneously inoculated with eight isolates of Leishmania parasites, KMA-2, KMA-4 and KMA-7 (isolated from Great Gerbils) and KMP-1, KMP-2 and KMP-3, KMP-4 and KMP-6 (isolated from sandflies), pathological and histological examination were performed. Except for KMA-4, the BALB/c mice were shown to be resistant to every isolate. In contrast, high infectivity of Leishmania isolates except KMP-1 and KMP-6 was shown in the experiments on Mongolian gerbils. Promastigotes were successfully re-isolated from the noses of the Mongolian gerbils infected with each isolates. The mini-exon genes were analyzed for four isolates established. Based on the comparison of the sequences with those of reference strains of L. major, L. gerbilli, and L. turanica, Leishmania parasites isolated from the great gerbils were identified as L. gerbilli (KMA-4) and L. turanica (KMA-2), and parasites isolated from sandflies were identified as L. gerbilli (KMP-3) and L. turanica (KMP-2). Less

今年在中华人民共和国的新兴南自治区进行了一项流行学研究。被陷阱捕获了十二个大的沙鼠（菱形俄罗斯），从洞穴中收集了101个雌性沙蝇。邮票涂片是由捕获的大沙鼠的Auricula，肝脏和脾脏制成的，用甲醇固定，并用Giemsa染色。固定了一部分Auricula，并用10％缓冲福尔马林缓冲并嵌入石蜡蜡中。进行切片并用苏木精和曙红染色。解剖砂蝇，并在显微镜下检查其肠道是否存在前鞭毛脂肪。获得EH前寄生虫中的沙氟肠道转移到NNN培养基的管中，以分离寄生虫。结果，从三种沙蝇中获得了六种利什曼原虫分离株。从三个香鼠的auricula中建立了三种利什曼原虫寄生虫。在对Naturalli感染的伟大GER的组织病理学检查中……更多的bils，在Auricula中观察到amastigotes，但在脾脏和肝脏中却没有观察到。未获得重大的病理变化。八只近交杂交BALB/c小鼠和八只蒙古语沙鼠被八个分离株与利什曼原虫寄生虫分离株，KMA-2，KMA-4和KMA-7（从Gerbils中分离出来），KMP-1，KMP-2和KMP-2和KMP-3和KMP-3，KMP-4和KMP-4和KMP-6（kmp-3，kmp-4和KMP-6（kmp-3）（Sandolotic）和Sand和KMP-6（Sandolotic）均可进行调查。除KMA-4外，BALB/C小鼠对每个分离物具有抗性。相比之下，蒙古沙鼠的实验中显示了除KMP-1和KMP-6以外的Leishmania分离株的高感染。从感染了每个分离株的蒙古晶状体的鼻子中成功地重新分配了前鞭毛脂肪。分析了建立的四个分离株的小型外结果基因。基于将L. Major，L。Gerbili和L. turanica的序列与参考菌株的比较，从大沙鼠中分离的利什曼原虫寄生虫被鉴定为L. gerbili（KMA-4）（KMA-4）（KMA-4）和L. turanica（KMA-2），以及与Sandflie（KMA-2）的L. l. turanica（KMA-2），以及与Sandflies隔离的Turan and l. l. gerbili（l. kmpili and kerbili and kmpili and。 （KMP-2）。较少的

项目成果

L.R.Guan: "Leishmaniasis in the sest China" Proceeding of XIV International Congress for Tropical Medicine and Malaria. 361 (1996)

L.R.Guan：“中国东部的利什曼病”第十四届国际热带医学和疟疾大会论文集。

Yoshitsugu Matsumoto, Toshimitsu Naya: "Differentiation of Th cells in Leishmaniasis"Igakunoayumi.. 183(4). 257-261 (1997)

Yoshitsugu Matsumoto、Toshimitsu Naya：“利什曼病中 Th 细胞的分化”Igakunoayumi.. 183(4)。

松本芳嗣: "リーシュマニア症におけるサイトカインと病態"獣医畜産新報 JVM. 50(2). 165-170 (1997)

Yoshitsugu Matsumoto：“利什曼病的细胞因子和病理学”兽医和牲畜杂志 JVM 50(2) (1997)。

松本 芳嗣: "リーシュマニア症におけるサイトカインと病態" 獣医畜産新報 JVM. 50 (2). 165-170 (1997)

Yoshitsugu Matsumoto：“利什曼病的细胞因子和病理学”兽医和牲畜杂志 JVM 50 (2) (1997)。

J.J.Chai,X.P.Zho,Y.Lei H.Yan-yan et al.: "Preliminary vaccination trials against visceral leishmaniasis in a rodent model indigenous to nortwestern regions of China" Proceeding of XIV International Congress for Tropical Medicine and Malaria. 359 (1996)

J.J.Chai，X.P.Zho，Y.Lei H.Yan-yan等：“中国西北地区本土啮齿动物模型针对内脏利什曼病的初步疫苗接种试验”第十四届国际热带医学与疟疾大会论文集。