Mechanisms of disease symptom expression by potexviruses

马铃薯病毒表达疾病症状的机制

基本信息

批准号：
16380033
负责人：
UGAKI Masashi
金额：
$ 4.99万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

项目摘要

Upon infection to plant hosts, plant viruses cause vast varieties of disease symptoms including mosaic, yellows, chlorosis, ring spot, mottle, and so on. Molecular mechanisms underlying expression of these symptoms, however, have scarcely been uncovered. In this project, I studied the mechanism using two species of plant viruses belonging to Genus Potexvirus. Potato virus X (PVX) and Plantago asiatica mosaic virus (PIAMV). The strains OS and BH of PVX cause ring spot and mosaic, respectively, upon infection to tobacco plant (Nicotiana tabacum). Construction of chimeric genomes of the two strains and site-directied mutagenesis of the viral genomes revealed that the 58th nucleotide of the 5 prime untranslated region of the viral genome was responsible for determination of distinct symptoms. Distribution of viral RNA of each PVX strain, as well as transcription of host defense genes, was monitored in the infected leaves by in situ hybridization technique. In the area of ring spot, some plant genes related to the hypersensitive response (HR) such as pathogenesis-related protein 1a (PR-1a) were expressed. Viral RNA was found both inside and outside of the area of ring spot. These data suggest that the ring spot symptom caused by strain OS is a result of induced HR-like plant cell death yet unsuccessful in restricting the spread of the virus. The strains Li1 and Li6 of PlAMV cause systemic necrosis and mosaic, respectively, upon infection to Nicotiana benthamiana plants. Molecular analysis revealed that the 1154th amino acid of the viral replicase encoded in the PlAMV genome was responsible for the symptom determination. HR-like response was also observed in plants infected with the strain Li1. These data support the idea that some symptoms such as ring spot and systemic necrosis are caused by host plant responses which tried to restrict viral spread.

感染植物宿主后，植物病毒会引起各种各样的疾病症状，包括马赛克，黄色，绿化，环斑，斑点，斑点等。但是，几乎没有发现这些症状表达的分子机制。在这个项目中，我使用属于Potexvirus属的两种植物病毒研究了该机制。马铃薯病毒X（PVX）和Plantago Asiatica Mosaic病毒（PIAMV）。 PVX的菌株OS和BH分别导致烟草植物（Nicotiana tabacum）感染后的环斑和马赛克。病毒基因组的两种菌株的嵌合基因组和位置定向诱变表明，病毒基因组的5个原始未翻译区的第58个核苷酸是负责确定不同症状的。通过原位杂交技术监测每个PVX菌株的病毒RNA的分布以及宿主防御基因的转录。在环斑区域，表达了一些与超敏反应（HR）有关的植物基因，例如发病机理相关的蛋白1a（PR-1A）。在环斑区域内外发现病毒RNA。这些数据表明，由菌株OS引起的环斑症状是诱导HR样植物细胞死亡的结果，但在限制病毒的扩散方面失败了。 PLAMV的Li1和Li6菌株在感染烟熏烟草本植物后分别引起全身性坏死和马赛克。分子分析表明，在PLAMV基因组中编码的病毒复制酶的第1154氨基酸是症状测定的原因。在感染LI1菌株的植物中还观察到了HR样反应。这些数据支持这样的想法：某些症状（例如环点和全身性坏死）是由试图限制病毒传播的宿主植物反应引起的。

项目成果

期刊论文数量（12）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

A single amino acid in the RNA-dependent RNA polymerase of Plantago asiatica mosaic virus contributes to systemic necrosis

车前子花叶病毒RNA依赖性RNA聚合酶中的单个氨基酸导致系统性坏死

DOI：
发表时间：
2006
期刊：
Archives of Virology 151
影响因子：
0
作者：
Ozeki;J.;Takahashi;S.;Komatsu;K.;Kagiwada;S.;Yamashita;K.;Mori;T.;Hirata;H.;YamaJi;Y.;Ugaki;M.;Namba;S.
通讯作者：
S.

An antibody against the SecA membrane protein of one phytoplasma reacts with those of phylogenetically different phytoplasmas

针对一种植原体 SecA 膜蛋白的抗体与系统发育不同植原体的抗体发生反应

DOI：
发表时间：
2004
期刊：
Phytopathology 94
影响因子：
0
作者：
Wei;W.et al.
通讯作者：
W.et al.

In planta dynamic analysis of onion yellows phytoplasma using localized inoculation by insect transmission

利用昆虫传播局部接种对洋葱黄化植原体进行植物动态分析

DOI：
发表时间：
2004
期刊：
Phytopathology 94
影响因子：
0
作者：
Wei;W.et al.
通讯作者：
W.et al.

The efficiency of interference of Potato virus X infection depends on the target gene

马铃薯X病毒感染的干扰效率取决于目标基因

DOI：
发表时间：
2006
期刊：
Virus Research 116
影响因子：
0
作者：
Ozeki;J.;Takahashi;S.;Komatsu;K.;Kagiwada;S.;Yamashita;K.;Mori;T.;Hirata;H.;Yamaji;Y.;Ugaki;M.;Namba;S.;Takahashi et al.
通讯作者：
Takahashi et al.

Secretion of immunodominant membrane protein from onion yellows phytoplasma through the Sec protein-translocation system in Escherichia coli