Mechanisms of disease symptom expression by potexviruses

马铃薯病毒表达疾病症状的机制

基本信息

批准号：
16380033
负责人：
UGAKI Masashi
金额：
$ 4.99万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

项目摘要

Upon infection to plant hosts, plant viruses cause vast varieties of disease symptoms including mosaic, yellows, chlorosis, ring spot, mottle, and so on. Molecular mechanisms underlying expression of these symptoms, however, have scarcely been uncovered. In this project, I studied the mechanism using two species of plant viruses belonging to Genus Potexvirus. Potato virus X (PVX) and Plantago asiatica mosaic virus (PIAMV). The strains OS and BH of PVX cause ring spot and mosaic, respectively, upon infection to tobacco plant (Nicotiana tabacum). Construction of chimeric genomes of the two strains and site-directied mutagenesis of the viral genomes revealed that the 58th nucleotide of the 5 prime untranslated region of the viral genome was responsible for determination of distinct symptoms. Distribution of viral RNA of each PVX strain, as well as transcription of host defense genes, was monitored in the infected leaves by in situ hybridization technique. In the area of ring spot, some plant genes related to the hypersensitive response (HR) such as pathogenesis-related protein 1a (PR-1a) were expressed. Viral RNA was found both inside and outside of the area of ring spot. These data suggest that the ring spot symptom caused by strain OS is a result of induced HR-like plant cell death yet unsuccessful in restricting the spread of the virus. The strains Li1 and Li6 of PlAMV cause systemic necrosis and mosaic, respectively, upon infection to Nicotiana benthamiana plants. Molecular analysis revealed that the 1154th amino acid of the viral replicase encoded in the PlAMV genome was responsible for the symptom determination. HR-like response was also observed in plants infected with the strain Li1. These data support the idea that some symptoms such as ring spot and systemic necrosis are caused by host plant responses which tried to restrict viral spread.

植物病毒感染植物宿主后，会引起花叶病、黄化、失绿、环斑病、斑驳病等多种病害症状。然而，这些症状表达背后的分子机制却几乎未被揭示。在这个项目中，我使用属于马铃薯病毒属的两种植物病毒研究了其机制。马铃薯 X 病毒 (PVX) 和车前草花叶病毒 (PIAMV)。 PVX 菌株 OS 和 BH 在感染烟草植物（Nicotiana tabacum）后分别引起环斑和花叶。两种毒株的嵌合基因组的构建和病毒基因组的定点诱变表明，病毒基因组的 5''非翻译区的第 58 个核苷酸负责确定不同的症状。通过原位杂交技术监测受感染叶子中每个 PVX 菌株的病毒 RNA 分布以及宿主防御基因的转录。在环斑区域，表达了一些与过敏反应（HR）相关的植物基因，如发病相关蛋白1a（PR-1a）。在环斑区域的内部和外部均发现了病毒RNA。这些数据表明，OS菌株引起的环斑症状是诱导HR样植物细胞死亡但未能成功限制病毒传播的结果。 PlaAMV 菌株 Li1 和 Li6 在感染本塞姆氏烟草植物后分别引起系统性坏死和花叶。分子分析表明，PlAMV 基因组中编码的病毒复制酶的第 1154 个氨基酸负责症状确定。在感染 Li1 菌株的植物中也观察到类似 HR 的反应。这些数据支持这样的观点，即环斑和系统性坏死等一些症状是由宿主植物试图限制病毒传播的反应引起的。

项目成果

期刊论文数量（12）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

A single amino acid in the RNA-dependent RNA polymerase of Plantago asiatica mosaic virus contributes to systemic necrosis

车前子花叶病毒RNA依赖性RNA聚合酶中的单个氨基酸导致系统性坏死

DOI：
发表时间：
2006
期刊：
Archives of Virology 151
影响因子：
0
作者：
Ozeki;J.;Takahashi;S.;Komatsu;K.;Kagiwada;S.;Yamashita;K.;Mori;T.;Hirata;H.;YamaJi;Y.;Ugaki;M.;Namba;S.
通讯作者：
S.

An antibody against the SecA membrane protein of one phytoplasma reacts with those of phylogenetically different phytoplasmas

针对一种植原体 SecA 膜蛋白的抗体与系统发育不同植原体的抗体发生反应

DOI：
发表时间：
2004
期刊：
Phytopathology 94
影响因子：
0
作者：
Wei;W.et al.
通讯作者：
W.et al.

In planta dynamic analysis of onion yellows phytoplasma using localized inoculation by insect transmission

利用昆虫传播局部接种对洋葱黄化植原体进行植物动态分析

DOI：
发表时间：
2004
期刊：
Phytopathology 94
影响因子：
0
作者：
Wei;W.et al.
通讯作者：
W.et al.

The efficiency of interference of Potato virus X infection depends on the target gene

马铃薯X病毒感染的干扰效率取决于目标基因

DOI：
发表时间：
2006
期刊：
Virus Research 116
影响因子：
0
作者：
Ozeki;J.;Takahashi;S.;Komatsu;K.;Kagiwada;S.;Yamashita;K.;Mori;T.;Hirata;H.;Yamaji;Y.;Ugaki;M.;Namba;S.;Takahashi et al.
通讯作者：
Takahashi et al.

Phylogenetic characteristics, genomic heterogeneity and symptomatic variation of five closely related Japanese strains of Potato virus X