FIBER DIFFRACTION FROM FILAMENTOUS PLANT VIRUSES

丝状植物病毒的纤维衍射

• 批准号: 7601739
• 负责人: GERALD J STUBBS
• 金额: $ 2.06万
• 依托单位: ILLINOIS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7601739
• 关键词: Biotechnology; Computer Retrieval of Information on Scientific Projects Database; Data; Development; Family; Fiber; Filament; Funding; Glycine max; Grant; Humidity; Institution; Methods; Modeling; Mosaic Viruses; Necrosis; Pattern; Plant Viruses; Pliability; Potato Virus X; Potyvirus; Preparation; Research; Research Personnel; Resolution; Resources; Sampling; Source; Soybeans; Specimen; Structure; Tobacco Mosaic Virus; United States National Institutes of Health; Viral; Virus; bean; improved; member; method development; three dimensional structure

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are collecting fiber diffraction data from flexible filamentous plant viruses, invaluable as models for recognition, assembly, flexibility, viral-host interactions, and in methods development. Plant viruses have great potential in biotechnology, but development will require three-dimensional structures. Fiber diffraction is often the only method available for the determination of such filamentous structures. Diffraction samples are oriented sols and dried fibers. We find that order is better in dried fibers of highly flexible filaments, and in oriented sols of more rigid filaments. Dried fibers require much less material, an important consideration for studies of low-titer viruses. Wide and small angle diffraction data are collected at BioCAT. We have obtained fiber diffraction data from bean common mosaic virus and bean common mosaic necrosis virus, significantly improved both in orientation and in resolution by the use of high humidities in specimen preparation. We have obtained even better patterns from two strains of the related potyvirus soybean mosaic virus (SMV); these are the first diffraction data obtained from SMV, and the best diffraction data ever obtained from a member of the important Potyvirus family. They will be invaluable in determining high-resolution potyvirus structures. We have obtained excellent diffraction patterns from oriented sols of tobacco mosaic virus and potato virus X, made using a new method that requires less than 5% of the material needed for conventional methods, and involves much less handling . The method will be extremely useful in fiber diffraction studies when samples are limited in quantity, labile, or hazardous.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 我们正在从灵活的丝状植物病毒中收集纤维衍射数据，这是识别，组装，灵活性，病毒宿主相互作用以及方法开发的模型的宝贵模型。植物病毒在生物技术方面具有巨大的潜力，但是开发将需要三维结构。纤维衍射通常是确定这种丝状结构的唯一方法。 衍射样品是定向的溶胶和干纤维。我们发现，在高灵性丝的干燥纤维和更刚性丝的定向溶胶中，顺序更好。干纤维所需的材料需要少得多，这是对低细胞病毒研究的重要考虑因素。在BioCat上收集宽和小角度衍射数据。 我们已经从Bean Comma Mosaic病毒和Bean Comma Mosaic坏死病毒中获得了纤维衍射数据，通过在样品制备中使用高湿度，可以显着改善方向和解决方案。我们从相关的大豆摩西病毒（SMV）的两种菌株中获得了更好的模式。这些是从SMV获得的第一个衍射数据，也是从重要的pot病毒家族成员获得的最佳衍射数据。它们将在确定高分辨率托管病毒结构方面非常宝贵。 我们从定向烟草病毒和马铃薯病毒X的定向溶胶中获得了出色的衍射图，该模式使用新方法制造，该方法需要少于常规方法所需的材料的5％，并且涉及处理少得多。当样品的数量，不稳定或危险性限制时，该方法在纤维衍射研究中将非常有用。