FIBER DIFFRACTION FROM FILAMENTOUS PLANT VIRUSES

丝状植物病毒的纤维衍射

• 批准号: 7601739
• 负责人: GERALD J STUBBS
• 金额: $ 2.06万
• 依托单位: ILLINOIS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7601739
• 关键词: Biotechnology; Computer Retrieval of Information on Scientific Projects Database; Data; Development; Family; Fiber; Filament; Funding; Glycine max; Grant; Humidity; Institution; Methods; Modeling; Mosaic Viruses; Necrosis; Pattern; Plant Viruses; Pliability; Potato Virus X; Potyvirus; Preparation; Research; Research Personnel; Resolution; Resources; Sampling; Source; Soybeans; Specimen; Structure; Tobacco Mosaic Virus; United States National Institutes of Health; Viral; Virus; bean; improved; member; method development; three dimensional structure

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are collecting fiber diffraction data from flexible filamentous plant viruses, invaluable as models for recognition, assembly, flexibility, viral-host interactions, and in methods development. Plant viruses have great potential in biotechnology, but development will require three-dimensional structures. Fiber diffraction is often the only method available for the determination of such filamentous structures. Diffraction samples are oriented sols and dried fibers. We find that order is better in dried fibers of highly flexible filaments, and in oriented sols of more rigid filaments. Dried fibers require much less material, an important consideration for studies of low-titer viruses. Wide and small angle diffraction data are collected at BioCAT. We have obtained fiber diffraction data from bean common mosaic virus and bean common mosaic necrosis virus, significantly improved both in orientation and in resolution by the use of high humidities in specimen preparation. We have obtained even better patterns from two strains of the related potyvirus soybean mosaic virus (SMV); these are the first diffraction data obtained from SMV, and the best diffraction data ever obtained from a member of the important Potyvirus family. They will be invaluable in determining high-resolution potyvirus structures. We have obtained excellent diffraction patterns from oriented sols of tobacco mosaic virus and potato virus X, made using a new method that requires less than 5% of the material needed for conventional methods, and involves much less handling . The method will be extremely useful in fiber diffraction studies when samples are limited in quantity, labile, or hazardous.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 中心，不一定是研究者的机构。 我们正在收集来自柔性丝状植物病毒的纤维衍射数据，这些数据作为识别、组装、灵活性、病毒-宿主相互作用和方法开发的模型非常有价值。植物病毒在生物技术方面具有巨大潜力，但开发需要三维结构。纤维衍射通常是确定此类丝状结构的唯一可用方法。 衍射样品是定向溶胶和干燥纤维。我们发现，高柔性长丝的干燥纤维和刚性长丝的定向溶胶的有序性更好。干燥的纤维需要的材料少得多，这是低滴度病毒研究的一个重要考虑因素。广角和小角度衍射数据在 BioCAT 收集。 我们获得了菜豆常见花叶病毒和菜豆常见花叶坏死病毒的纤维衍射数据，通过在样品制备中使用高湿度，方向和分辨率显着提高。我们从相关的马铃薯Y病毒大豆花叶病毒（SMV）的两个菌株中获得了更好的模式；这是从 SMV 获得的第一个衍射数据，也是从重要的马铃薯病毒科成员获得的最佳衍射数据。它们对于确定高分辨率马铃薯Y病毒结构具有无价的价值。 我们从烟草花叶病毒和马铃薯病毒 X 的定向溶胶中获得了出色的衍射图案，这些溶胶采用新方法制成，所需材料少于传统方法所需材料的 5%，并且涉及的处理也少得多。当样品数量有限、不稳定或危险时，该方法在纤维衍射研究中将非常有用。