Development of a caries risk assessment system using genetic polymorphisms influencing cariogenicities of mutans streptococci.

利用影响变形链球菌致龋性的遗传多态性开发龋齿风险评估系统。

• 批准号: 15390649
• 负责人: MIYAZAKI Hideo
• 金额: $ 3.52万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15390649/
• 关键词: Streptococcus mustans; genetic polymorphism; water-insoluble glucan; ミュータンスレンサ球菌; 齲蝕; リスク診断; グルカン合成能; リスク評価; Streptococcus mustans; genetic polymorphism; water-insoluble glucan; ミュータンスレンサ球菌; 齲蝕; リスク診断; グルカン合成能; リスク評価

Streptococcus mutans is considered as strongly associated with dental caries. It was reported that children carrying this bacteria had significantly higher caries prevalence and development. S.mutans can colonize and accumulate on the tooth surface by glucan synthesis. GTF-I coded by gtfB gene is the most important enzyme on water-insoluble glucan (WIG) synthesis among three glucosyltransferase of S.mutans. The aim of this study was to investigate the association between genetic diversity of gtfB and WIG synthesis.We collected paraffin-stimulated saliva from primary schoolchildren, and 21 clinical strains of S.mutans were isolated from these saliva samples. Base sequences of gtfB of these strains were determined by direct sequencing analysis.In these days, it was revealed that GTF-I has ANDVDNSNPVVQAEQLNWL (19 aa) at 409-427 and DSIRVDAVD (9 aa) at 446-454, and these sequences are very important for enzyme activity of GTF-I. There are direct repeating units in 3'-terminal of gtfB, which are glucan binding domain. This repeating units are also important for glucan synthesis.In this study, it was revealed that the sequence preceding the coding region of gtf B and enzymatic catalysis position (409-427 and 446-454) are highly conserved among the clinical isolates and that some strains whose WIG synthesis are low have deletion of direct repeating units in C-terminal of GTF-I.

链球菌突变被认为与龋齿密切相关。据报道，携带这种细菌的儿童的龋齿患病率和发育率明显更高。 S.Mutans可以通过葡萄糖合成在牙齿表面定居并积聚。 GTF-1由GTFB基因编码是S.Mutans三种葡萄糖基转移酶之间水不溶的葡萄糖（WIG）合成的最重要酶。这项研究的目的是研究GTFB的遗传多样性与假发合成之间的关联。我们从初级学童中收集了石蜡刺激的唾液，并从这些唾液样本中分离出21种s.mutans的临床菌株。这些菌株的GTFB的基本序列是通过直接测序分析确定的。如今，发现GTF-I具有409-427的andVDNSNSNPVVQAEQLNWL（19 AA），在446-454和这些序列对Enzyme非常重要。 GTFB的3'-末端有直接重复单元，它们是葡聚糖结合域。在这项研究中，这种重复单元对于葡萄糖合成也很重要。据表明，GTF B和酶促催化位置的编码区域之前的序列（409-427和446-454）在临床隔离株中高度保守，并且在直接重复单位中删除了WIG合成的一些菌株中的某些菌株。