Generation of bioactive peptides from meat proteins during aging and intestinal digestion

在老化和肠道消化过程中从肉蛋白中产生生物活性肽

基本信息

批准号：
15380187
负责人：
ARIHARA Keizo
金额：
$ 8.58万
依托单位：
Kitasato University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

This research project has been conducted to investigate the generation of bioactive peptides from meat proteins by proteases during meat aging and intestinal digestion. Since our studies showed an increase in bioactivities (e.g., ACE inhibitory activity) of beef and pork products during aging or strage, the generation of bioactive peptides was onfirmed to occur by aging or storage. Many bioacitive peptides (e.g., antihypertensive peptides, antioxidative peptides, prebiotic peptides, opioide peptides) derived from meat proteins have been identified in this study. For example, several novel antioxidative peptides generated from pork proteins were discovered. Of these antioxidative peptides, the peptide, which showed the highest activity, was Asp-Leu-Tyr-Ala. This peptide also showed some remarkable activities (e.g., anti-fatigue activity) in vivo. Since the mixture of four individual amino acids did not show the biological activity, the tetra peptide-structure of Asp-Leu-Tyr-Ala is critical for its activity. By the treatment of digestive tract enzymes, Asp-Leu-Tyr-Ala generated tripeptide (Leu-Tr-Ala). Leu-Try-Ala showed similar activities with Asp-Leu-Tyr-Ala both in vitro and in vivo experiments. In another experiment, a novel bioactive peptide, Glu-Leu-Met was identified in the pork protein digest. Glu-Leu-Met is the first prebiotic (Bifidobacterium growth-promoting) peptide generated from food proteins by enzymatic digestion. Since this peptide is highly resistant to digestive enzymes, it would be a promising prebiotic component. As bioactive peptides would be liberated from food proteins during gastrointestinal proteolysis, further studies are now in progress to identify the generation of such peptides from meat proteins in the intestinal tracts.

该研究项目旨在研究肉类老化和肠道消化过程中蛋白酶通过肉类蛋白质产生生物活性肽的情况。由于我们的研究表明牛肉和猪肉产品在老化或储存过程中生物活性（例如ACE抑制活性）增加，因此证实了生物活性肽的产生是通过老化或储存而发生的。本研究已鉴定出许多源自肉类蛋白的生物活性肽（例如抗高血压肽、抗氧化肽、益生元肽、阿片肽）。例如，发现了几种从猪肉蛋白质中产生的新型抗氧化肽。在这些抗氧化肽中，表现出最高活性的肽是Asp-Leu-Tyr-Ala。该肽在体内也表现出一些显着的活性（例如抗疲劳活性）。由于四种单独氨基酸的混合物不显示生物活性，因此 Asp-Leu-Tyr-Ala 的四肽结构对其活性至关重要。通过消化道酶的处理，Asp-Leu-Tyr-Ala生成三肽（Leu-Tr-Ala）。 Leu-Try-Ala 在体外和体内实验中均表现出与 Asp-Leu-Tyr-Ala 相似的活性。在另一项实验中，在猪肉蛋白消化物中鉴定出了一种新型生物活性肽 Glu-Leu-Met。 Glu-Leu-Met 是第一个通过酶消化从食物蛋白质中产生的益生元（双歧杆菌生长促进）肽。由于这种肽对消化酶具有高度抵抗力，因此它可能是一种很有前途的益生元成分。由于生物活性肽会在胃肠道蛋白水解过程中从食物蛋白中释放出来，因此目前正在进行进一步的研究，以确定肠道中肉类蛋白中产生此类肽的情况。