STUDIES ON IGF-I SIGNAL CASCADE ON MUSCLE PROTEIN RETENTION

IGF-I 信号级联对肌肉蛋白保留的研究

基本信息

批准号：
15380194
负责人：
KITA Kazumi
金额：
$ 7.49万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

Influence of methionine and/or glycine deficiency on protein synthesis of chicken embryo myoblasts cultured in Medium 199 was examined. Protein synthesis cultured in Medium 199 in which all ingredients were solved in Dulbecco's modified phosphate buffered saline was dramatically lower than that in Medium 199 in which all ingredients were solved in water. Although methionine deficiency from Medium 199 decreased myoblast protein synthesis, glycine deficiency did not affect. There was no interaction between methionine and glycine deficiency on myoblast protein synthesis.The influence of leucine and fetal calf serum (FCS) in Medium 199 on protein synthesis and degradation of chicken embryo myoblasts was examined. Medium 199 included 0, 1, 2, 5, 10% FCS and 457 (original concentration of leucine in Medium 199), 2287, 4574μmol leucine/l. There was an interaction between leucine and FCS concentrations on muscle protein synthesis in vitro. When FCS was not included in Medium 199, myoblast prot … More ein synthesis was stimulated with an increment in leucine concentration in the medium from 2287 to 4574 μmol/l. When 1 or 2% of FCS was included in Medium 199, myoblast protein synthesis was stimulated by increasing leucine concentration in the medium from 457 to 2287 μmol/l, and at the higher level of leucine, protein synthesis was not stimulated. At more than 5% of FCS in the medium, myoblast protein synthesis at higher concentration of luecine of 4574μmol/l was significantly lower than those of lower leucine levels.No interaction between leucine and FCS concentrations on muscle protein degradation Was observed. Protein degradation of chicken embryo myoblasts was decreased by an increment of FCS concentration in the medium from 0 to 5%, and at the higher level no significant influence was observed. When leucine concentration of in the medium was increased from 457 to 2287μmol/l, protein degradation of chicken embryo myoblasts was decreased. But high concentration of leucine at 4457μmol/l did not affect protein degradation of chicken embryo myoblasts. Less

检测甲硫氨酸和/或甘氨酸缺乏对在培养基199中培养的鸡胚成肌细胞的蛋白质合成的影响。在所有成分均溶解在杜尔贝科改良磷酸盐缓冲盐水中的培养基199中培养的蛋白质合成显着低于在其中的培养基199中培养的蛋白质合成。尽管培养基199中的蛋氨酸缺乏会降低成肌细胞蛋白质的合成，但甘氨酸缺乏不会影响它们之间的相互作用。检测培养基199中亮氨酸和胎牛血清(FCS)对鸡胚成肌细胞蛋白质合成和降解的影响，培养基199包括0、1、2、5、10％FCS和10％FCS。 457(培养基199中亮氨酸的原始浓度)、2287、4574μmol亮氨酸/l。亮氨酸和 FCS 浓度对体外肌肉蛋白质合成的相互作用当培养基 199 中不含 FCS 时，培养基中亮氨酸浓度从 2287 μmol/l 增加到 4574 μmol/l 会刺激成肌细胞蛋白合成。或2％的FCS包含在培养基199中，通过将培养基中的亮氨酸浓度从457增加至457来刺激成肌细胞蛋白质合成。 2287μmol/l，并且在较高水平的亮氨酸下，蛋白质合成不受刺激，在培养基中超过5%的FCS时，在较高浓度4574μmol/l的亮氨酸下，成肌细胞蛋白质合成显着低于较低亮氨酸。没有观察到亮氨酸和FCS浓度对肌肉蛋白质降解的相互作用，随着培养基中FCS浓度从0增加到鸡胚成肌细胞的蛋白质降解。 5%，当培养基中的亮氨酸浓度从457μmol/l增加到2287μmol/l时，没有观察到显着的影响，但在4457μmol/l的高浓度亮氨酸则没有观察到显着影响。对鸡胚成肌细胞蛋白质降解影响较小。